EZRIN/RADIXIN/MOESIN, RBX-100UL

货号： AB3843      产品名称： EZRIN/RADIXIN/MOESIN, RBX-100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Ezrin/Radixin/Moesin Antibody
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REFERENCES
Nuclear reprogramming of somatic cells by embryonic stem cells is affected by cell cycle stage.
Stephen Sullivan, Steve Pells, Martin Hooper, Ed Gallagher, Jim McWhir (2006) Cloning and stem cells.8:174-88
Species Reactivity Key Applications Host Format Antibody Type
H, M, Mk, R  WB, ICC, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Ezrin/Radixin/Moesin Antibody
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Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Ezrin/Radixin/Moesin. These proteins function as linkers between the plasma membrane and the actin cytoskeleton, and are involved in cell adhesion, membrane ruffling, and microvilli formation. This antibody does not cross-react with other related proteins such as merlin or band 4.1.
Immunogen:
KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.
Species Reactivity:
Human
Mouse
Monkey
Rat
Application Notes:
Western blotting: 1:1,000. The 75 kDa band is Moesin and the 80kDa band is Ezrin and Radixin. (For best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)
Immunohistochemistry (Paraffin): 1:50. Positive control tissue is breast carcinoma. Cellular localization is membrane and cytoplasm
Immunocytochemistry (IF): 1:100
Optimal working dilutions must be determined by the end user.
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Presentation:
Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.
Storage Conditions:
Maintain at -20ºC for 12 months, do not aliquot.
UniProt Number:
O14745
Entrez Gene Number:
NM_004252.2
Gene Symbol:
SLC9A3R1
+exchanger)
NHERF1
+H
NHERF
Na
NHERF-1
EBP50
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
Members of the ezrin (VIL2; MIM 123900)-radixin (RDX; MIM 179410)-moesin (MSN; MIM 309845) (ERM) protein family are highly concentrated in the apical aspect of polarized epithelial cells. These cells are studded with microvilli containing bundles of actin filaments, which must attach to the membrane to assemble and maintain the microvilli. The ERM proteins, together with merlin, the NF2 (MIM 607379) gene product, are thought to be linkers between integral membrane and cytoskeletal proteins, and they bind directly to actin in vitro. Actin cytoskeleton reorganization requires the activation of a sodium/hydrogen exchanger (SLC9A3; MIM 182307). SLC9A3R1 is an ERM-binding protein.[supplied by OMIM]
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UniProt Summary:
FUNCTION: SwissProt: O14745 # Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediat -20℃