anti-MEK1 (C-term)

货号： 04-376      产品名称： anti-MEK1 (C-term)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-MEK1 CT Antibody, rabbit monoclonal
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REFERENCES
Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway.
Drosopoulos, Konstantinos G, et al. (2005) J. Biol. Chem., 280: 22856-67 (2005)
Activation of the NaCl- and drought-induced RD29A and RD29B promoters by constitutively active Arabidopsis MAPKK or MAPK proteins
Hua, Zhi-Ming, et al (2006) Plant Cell Environ, 29:1761-70 (2006)
p38 Mitogen-activated protein kinase pathway suppresses cell survival by inducing dephosphorylation of mitogen-activated protein/extracellular signal-regulated kinase kinase1,2
Li, Song-Ping, et al (2003) Cancer Res, 63:3473-7 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H, R  FC, ICC, IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-MEK1 CT Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the C-terminal domain of MEKK1.
Molecular Weight:
45 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human MEK1.
Isotype:
IgG
Species Reactivity:
Human
Rat
Application Notes:
Suggested dilutions:
Immunoblot Analysis: 1:10,000
Immunoprecipitation: 1:150
Flow Cytometry: 1:40
Immunohistochemistry: A 1:250
Immunfluorescence Staining: 1:250-1:500
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Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
Q02750
Entrez Gene Number:
NM_002755.2
Gene Symbol:
MAP2K1
PRKMK1
MKK1
MAPKK1
MEK1
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Alternate Names:
MAPKK1, MAP2K1, MKK1, PRKMK1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
View All »
Entrez Gene Summary:
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.< -20°C

anti-MEK2 (N-term), rb mono

货号： 04-377      产品名称： anti-MEK2 (N-term), rb mono   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-MEK2 NT Antibody, rabbit monoclonal
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REFERENCES
MEK1,2 response element mediates angiotensin II-stimulated plasminogen activator inhibitor-1 promoter activation.
Chen, Hong-Chi and Feener, Edward P (2004) Blood, 103: 2636-44 (2004)
Structures of human MAP kinase kinase 1 (MEK1) and MEK2 describe novel noncompetitive kinase inhibition
Ohren, Jeffrey F, et al (2004) Nat Struct Mol Biol, 11:1192-7 (2004)
A conserved docking site in MEKs mediates high-affinity binding to MAP kinases and cooperates with a scaffold protein to enhance signal transmission
Bardwell, A J, et al (2001) J Biol Chem, 276:10374-86 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H, R  FC, ICC, IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-MEK2 NT Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the N-terminal domain of MEK2.
Molecular Weight:
45 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the N-terminal region of human MEK2.
Isotype:
IgG
Species Reactivity:
Human
Rat
Application Notes:
Suggested dilutions:
Immunoblot Analysis: 1:10,000
Immunoprecipitation: 1:100
Flow Cytometry: 1:40
Immunohistochemistry: A 1:250-1:500
Immunfluorescence Staining: 1:250-1:500
View All »
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Serum
Presentation:
In 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
View All »
UniProt Number:
P36507
Entrez Gene Number:
NM_030662.2
Gene Symbol:
MAP2K2
MEK2
PRKMK2
MAPKK2
MKK2
View All »
Alternate Names:
MAP2K2, MAPKK2, MKK2, PRKMK2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
View All »
Entrez Gene Summary:
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
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UniProt Summary:
FUNCTION: SwissProt: P36507 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates th -20°C