Anti-acetyl-MeCP2 (Lys464)

货号： ABE28      产品名称： Anti-acetyl-MeCP2 (Lys464)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-MeCP2 (Lys464)
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REFERENCES
SIRT1-mediated deacetylation of MeCP2 contributes to BDNF expression.
Loredana Zocchi,Paolo Sassone-Corsi (2012) Epigenetics : official journal of the DNA Methylation Society.7
Species Reactivity Key Applications Host Format Antibody Type
M, H, R  DB, WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-acetyl-MeCP2 (Lys464) Antibody
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Specificity:
This antibody recognizes MeCP2 acetylated at Lysine 464.
Molecular Weight:
~75 kDa observed. Two isoforms (A and B) with calculated molecular weights of ~52 kDa and 53 kDa are known to exist. This antibody should detect either isoform.
Epitope:
Acetylated lysine 464
Immunogen:
KLH-conjugated linear peptide corresponding to mouse MeCP2 acetylated at Lys464.
Modifications:
Acetylation
Background Information:
DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.
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Species Reactivity:
Mouse
Human
Rat
Species Reactivity Note:
Demonstrated to react with Mouse. Predicted to react with Human and Rat based on 100% sequence homology.
Application Notes:
Western Blot Immunoprecipitation Analysis: A representative lot from an independent laboratory detected MeCP2 in 293 cell lysate derived from p300 co-transfected 293 cells. (Prof. Sassone-Corsi, Center for Epigenetics and Metabolism, University of California, Irvine.)
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected MeCP2 in mouse cortical neurons. (Prof. Sassone-Corsi, Center for Epigenetics and Metabolism, University of California, Irvine.)
Alexa Fluor® is a registered trademark of Molecular Probes Inc.
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Control:
Non-acetylated and acetylated MeCP2 (Lys464) peptides.
Quality Assurance:
Evaluated by Dot Blot using non-acetylated and acetylated MeCP2 (Lys464) peptides.
Dot Blot Analysis: 1 µg/mL of this antibody detected MeCP2 using non-acetylated and acetylated MeCP2 (Lys464) peptides.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9Z2D6
Entrez Gene Number:
NP_034918
Gene Symbol:
Mecp2
Alternate Names:
Methyl-CpG-binding protein 2
MeCp-2 protein
MeCp2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Dot Blot
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear prote 2-8℃