标签归档：mdm

04-1530   Anti-MDM2 (3G9)   品牌 Millipore

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Anti-MDM2 (3G9)

货号： 04-1530      产品名称： Anti-MDM2 (3G9)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-MDM2, clone 3G9
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PRODUCT FAMILY INFORMATION
MDM2 Antibodies
Millipore’s MDM2 antibodies demonstrate specificity against MDM2, nuclear phosphoprotein with multiple variants. See below for MDM2 antibodies, based on the expertise of Upstate & Chemicon.
Millipore’s MDM2 antibody demonstrates specificity against MDM2. See below for data and references for Millipore’s MDM2 antibodies. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H, M  WB, IP, ICC, IHC Mouse Purified Monoclonal Antibody
Description:
Anti-MDM2 Antibody, clone 3G9
Promotional Text:
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Specificity:
This antibody recognizes MDM2.
Molecular Weight:
~ 75 kDa
Epitope:
Unknown
Immunogen:
His-tagged recombinant protein corresponding to human MDM2.
Clone:
3G9
Background Information:
MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumor suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
Application Notes:
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)
Control:
MCF-7 cell lysate
Quality Assurance:
Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM2 on 10 µg of MCF-7 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q00987
Entrez Gene Number:
NP_002383.2
Gene Symbol:
MDM2
hdm2
HDMX
Hdm2
HDM2
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Alternate Names:
Double minute 2 protein
Mdm2 p53 binding protein homolog (mouse)
Mdm2, transformed 3T3 cell double minute 2, p53 binding protein
Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)
Oncoprotein Mdm2
double minute 2, human homolog of
p53-binding protein
mouse double minute 2 homolog
mouse double minute 2, human homolog of
p53-binding protein
p53-binding protein Mdm2
ubiquitin-protein ligase E3 Mdm2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Entrez Gene Summary:
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear 2°C – 8°C

04-1556   Anti-MDM4, clone 7A8   品牌 Millipore

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Anti-MDM4, clone 7A8

货号： 04-1556      产品名称： Anti-MDM4, clone 7A8   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-MDM4, clone 7A8
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB Mouse Purified Monoclonal Antibody
Description:
Anti-MDM4 Antibody, clone 7A8
Promotional Text:
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100% Performance Guaranteed
Specificity:
This antibody recognizes MDM4.
Molecular Weight:
~ 80/27 kDa Observed. There are multiple forms for MDM4. Up to 7 different variants have been reported. Full legth of MDM4 is ~80kDa. The short form of MDM4 (MDM4-S) migrates at approximately 17 and 27kDa. (R. Rallapalli, G. Strachan, B. Cho, W. Mercer‡, and DJ. Hall, 1999, A Novel MDMX Transcript, THE JOURNAL OF BIOLOGICAL CHEMISTRY, 274(12), 8299–8308)
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Epitope:
Unknown
Immunogen:
His-tagged recombinant protein corresponding to mouse MDM4.
Clone:
7A8
Isotype:
IgG2bκ
Background Information:
MDM4 (sometimes referred to as MDMX) is a member of the MDM family proteins. It is known to inhibit p53 and p73 mediated cell cycle arrest and apoptosis. MDM4 is amplified or overexpressed in 10-20% of tumors and 65% of retinoblastomas. The full-length protein has significant homology to MDM2 and can also bind to and inhibit p53. In both MDM2 and MDM4, this interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Other similar domains include the C-terminal RING finger motif. Although these proteins share similar domains, MDM2 and MDM4 are non-redundant p53 inhibitors, since normal levels of either protein does not compensate for the loss of the other. Recent evidence suggests complementary activities for these proteins with MDM4 regulating p53 activity, and MDM2 playing a key role in p53 stability.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Demonstrated to react with human and mouse.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM4 in 10 µg of HeLa cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O35618
Entrez Gene Number:
NP_032601
Gene Symbol:
MDM4
HDMX
MDMX
MRP1
Alternate Names:
Double minute 4 protein
MDM4-related protein 1
Mdm2-like p53-binding protein
Mdm4 p53 binding protein homolog (mouse)
Mdm4, transformed 3T3 cell double minute 4, p53 binding
protein
Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
Protein Mdmx
double minute 4, human homolog of
p53-binding protein
mouse double minute 4 homolog
mouse double minute 4, human homolog of
p53-binding protein
p53-binding protein
p53-binding protein Mdm4
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degra 2°C – 8°C

07-575   ANTI-MDM2 200UG   品牌 Millipore

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ANTI-MDM2 200UG

货号： 07-575      产品名称： ANTI-MDM2 200UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-MDM2 Antibody
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REFERENCES
The MDM2 C-terminal region binds to TAFII250 and is required for MDM2 regulation of the cyclin A promoter
Léveillard , T and Wasylyk, B (1997) J Biol Chem, 272:30651-61 (1997)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-MDM2 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Product Overview:
Anti-MDM2
Specificity:
MDM2
Molecular Weight:
76kDa
Immunogen:
peptide (C-AISETEENSDELSGERQRKRHK) corresponding to amino acids 164-185 of human MDM2.
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot in RIPA lysates from HeLa cells
Purification Method:
Protein A purfied
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Q00987
Entrez Gene Number:
NM_002392 and others
Gene Symbol:
MDM2
hdm2
Hdm2
HDMX
MGC71221
HDM2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.
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UniProt Summary:
FUNCTION: SwissProt: Q00987 # Inhibits TP53/p53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Functions as a ubiquitin ligase E3, in the presence of E1 and E2, toward p53 and itself. Permits the nuclear export of p53 and targets it for proteasome-mediated proteolysis.
SIZE: 491 amino acids; 55233 Da
SUBUNIT: Binds p53, p73, ARF(P14), ribosomal protein L5 and specifically to RNA. Can interact also with retinoblastoma protein (RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with p53 and WWOX. Interacts with USP7 and TBRG1. Isoform Mdm2-F does not interact with TP53/p53. Interacts with, and ubiquitinates HIV-1 Tat.
SUBCELLULAR LOCATION: Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins.
TISSUE SPECIFICITY: Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isof -20℃