标签归档：marcks

05-337   A HU MARCKS 200UL   品牌 Millipore

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A HU MARCKS 200UL

货号： 05-337      产品名称： A HU MARCKS 200UL   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-MARCKS Antibody, clone 2F12
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REFERENCES
20-Hydroxyeicosatetraenoic acid-induced vasoconstriction and inhibition of potassium current in cerebral vascular smooth muscle is dependent on activation of protein kinase C
Lange, A., et al (1997) J Biol Chem, 272:27345-52 (1997)
Species Reactivity Key Applications Host Format Antibody Type
B, H  IP, WB Mouse Ascites Monoclonal Antibody
Description:
Anti-MARCKS Antibody, clone 2F12
Promotional Text:
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Trade Name:
Upstate (Millipore)
Specificity:
MARCKS
Molecular Weight:
none given
Immunogen:
Purified recombinant human MARCKS; Epitope mapped to the carboxyl-terminal.
Clone:
2F12
Isotype:
IgG1
Species Reactivity:
Bovine
Human
Quality Assurance:
routinely evaluated by immunoblot on bovine brain extract
Purification Method:
Ascites
Presentation:
mouse ascites containing 0.05% sodium azide
Storage Conditions:
6 months at -20°C
UniProt Number:
P29966
Entrez Gene Number:
NM_002356
Gene Symbol:
MARCKS
80K-L
MACS
PRKCSL
FLJ14368
phosphomyristin
MRACKS
PKCSL
FLJ90045
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
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UniProt Summary:
FUNCTION: SwissProt: P29966 # MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
SIZE: 332 amino acids; 31555 Da
PTM: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
SIMILARITY: SwissProt: P29966 ## Belongs to the MARCKS family.
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Brand Family:
Upstate
Product Name:
Anti-MARCKS Antibody, clone 2F12
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 µL
Target / Localization:
Soma
Format:
Ascites
Host:
Mouse
Applications:
Anti-MARCKS Antibody, clone 2F12 detects level of MARCKS & has been published & validated for use in IP & WB.

-20°C

ALX-162-004-MC05   MARCKS Protein (151-175) (bovine brain)   品牌 Alexis

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MARCKS Protein (151-175) (bovine brain)

货号： ALX-162-004-MC05      产品名称： MARCKS Protein (151-175) (bovine brain)   品牌： Alexis 规格： 0.5 mg 三周 生化试验

MARCKS protein (bovine brain) (151-175)
Excellent PKC substrate 
Replaces Prod. #: BML-P117
Excellent substrate for protein kinase C (PKC) (Km = 20nM for purified rat brain PKC). The peptide consists of residues 151-175 of the myristoylated alanine-rich C-kinase substrate (MARCKS) protein phosphorylation site. Protein kinase C isotypes (including PKCε) phosphorylate serines 8, 12 and 19 of this peptide. It is a very weak substrate for cAMP- and cGMP-dependent protein kinase and CaM kinases I, II and III.
Product Specification
Sequence: H-Lys-Lys-Lys-Lys-Lys-Arg-Phe-Ser-Phe-Lys-Lys-Ser-Phe-Lys-Leu-Ser-Gly-Phe-Ser-Phe-Lys-Lys-Asn-Lys-Lys-OH
Formula: C147H243N41O31
MW: 3081.8
CAS: 125267-21-2
Formulation: Lyophilized
Purity: ≥98%
Appearance: White to off-white powder. 
Solubility: Soluble in water.
Long Term Storage: -20°C

-20°C

ALX-162-004-M001   MARCKS Protein (151-175) (bovine brain)   品牌 Alexis

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MARCKS Protein (151-175) (bovine brain)

货号： ALX-162-004-M001      产品名称： MARCKS Protein (151-175) (bovine brain)   品牌： Alexis 规格： 1 mg 三周 生化试验

MARCKS protein (bovine brain) (151-175)
Excellent PKC substrate 
Replaces Prod. #: BML-P117
Excellent substrate for protein kinase C (PKC) (Km = 20nM for purified rat brain PKC). The peptide consists of residues 151-175 of the myristoylated alanine-rich C-kinase substrate (MARCKS) protein phosphorylation site. Protein kinase C isotypes (including PKCε) phosphorylate serines 8, 12 and 19 of this peptide. It is a very weak substrate for cAMP- and cGMP-dependent protein kinase and CaM kinases I, II and III.
Product Specification
Sequence: H-Lys-Lys-Lys-Lys-Lys-Arg-Phe-Ser-Phe-Lys-Lys-Ser-Phe-Lys-Leu-Ser-Gly-Phe-Ser-Phe-Lys-Lys-Asn-Lys-Lys-OH
Formula: C147H243N41O31
MW: 3081.8
CAS: 125267-21-2
Formulation: Lyophilized
Purity: ≥98%
Appearance: White to off-white powder. 
Solubility: Soluble in water.
Long Term Storage: -20°C

-20°C

ALX-162-005-M001   MARCKS Protein (159-165) amide (bovine brain)   品牌 Alexis

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MARCKS Protein (159-165) amide (bovine brain)

货号： ALX-162-005-M001      产品名称： MARCKS Protein (159-165) amide (bovine brain)   品牌： Alexis 规格： 1 mg 三周 生化试验

MARCKS protein (bovine brain) (159-165) amide
PKC substrate
The peptide consists of residues 159-165 of the myristoylated alanine-rich C-kinase substrate (MARCKS) protein. It is sufficiently selective for protein kinase C (PKC) to permit specific assay of this enzyme in crude cell extracts and permeabilized cells.
Product Specification
Sequence: H-Phe-Lys-Lys-Ser-Phe-Lys-Leu-NH2
Formula: C45H73N11O8
MW: 896.1
Purity: ≥98%
Appearance: White lyophilized powder.
Solubility: Soluble in water.
Long Term Storage: -20°C

-20°C

ALX-162-005-M005   MARCKS Protein (159-165) amide (bovine brain)   品牌 Alexis

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MARCKS Protein (159-165) amide (bovine brain)

货号： ALX-162-005-M005      产品名称： MARCKS Protein (159-165) amide (bovine brain)   品牌： Alexis 规格： 5 mg 三周 生化试验

MARCKS protein (bovine brain) (159-165) amide
PKC substrate
The peptide consists of residues 159-165 of the myristoylated alanine-rich C-kinase substrate (MARCKS) protein. It is sufficiently selective for protein kinase C (PKC) to permit specific assay of this enzyme in crude cell extracts and permeabilized cells.
Product Specification
Sequence: H-Phe-Lys-Lys-Ser-Phe-Lys-Leu-NH2
Formula: C45H73N11O8
MW: 896.1
Purity: ≥98%
Appearance: White lyophilized powder.
Solubility: Soluble in water.
Long Term Storage: -20°C

-20°C

07-1238   Anti-phospho-MARCKS (Ser152/156) (rabbit polyclonal)   品牌 Millipore

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Anti-phospho-MARCKS (Ser152/156) (rabbit polyclonal)

货号： 07-1238      产品名称： Anti-phospho-MARCKS (Ser152/156) (rabbit polyclonal)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-MARCKS (Ser152/156) Antibody
Recommended Replacement for: 07-1238 is a recommended replacement for AB5879
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Special Shipping Offer on Antibodies
100% Performance Guaranteed
Species Reactivity Key Applications Host Format Antibody Type
R, H, M  WB, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-MARCKS (Ser152/156) Antibody
Replaces:
07-1238 is a recommended replacement for AB5879
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
MARCKS, phosphoSer152/156.
Molecular Weight:
~80 kDa
Epitope:
phosphoSer 152/156
Immunogen:
Synthetic peptide corresponding to amino acids surrounding the phosphoSer152/156 of rat MARCKS.
Modifications:
Phosphorylation
Isotype:
IgG
Background Information:
Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a widely expressed substrate of PKC. MARCKS is essential in neuronal development, as mice deficient in it die shortly after birth. Through its basic effector region, MARCKS associates with acidic membrane phospholipids, actin filaments, and calmodulin. Poly (ADP-ribose) also binds the basic effector region, blocking calmodulin binding, membrane association, and phosphorylation by PKC, suggesting that MARCKS may be an effector of the DNA-damage signal transduced by poly (ADP-ribose).
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Species Reactivity:
Rat
Human
Mouse
Species Reactivity Note:
Human, mouse and rat.
Application Notes:
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta
Optimal working dilutions must be determined by the end user.
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Control:
A431 Cell Lysate
Quality Assurance:
Routinely evaluated by Western Blot on EGF treated A431 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated MARCKS on 10 μg of EGF treated A431 lysates.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal IgG in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Storage Conditions:
Stable at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P29966
Entrez Gene Number:
NM_002356.5
Gene Symbol:
MARCKS
MACS
80K-L
PRKCSL
FLJ14368
phosphomyristin
MRACKS
PKCSL
FLJ90045
View All »
Alternate Names:
Myristoylated alanine-rich C-kinase substrate
Protein kinase C substrate, 80 kDa protein, light chain
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunohistochemistry -20°C