LAR Tyrosine Phosphatase Assay Kit for Drug Discovery

货号： BML-AK815-0001      产品名称： LAR Tyrosine Phosphatase Assay Kit for Drug Discovery   品牌： Biomol 规格： Kit 3周到货 生化试验

LAR (Leukocyte Antigen Related) protein tyrosine phosphatase (PTP) is a receptor-like PTP implicated in neuronal development, regulation of cell-cell adhesion and modulation of insulin signaling. This latter aspect of LAR function and the possibility that it may contribute to insulin resistance in obesity and type II diabetes has stimulated intrest in LAR as a drug discovery target. LAR PTP is active in dephosphorylation of the insulin receptor (IR) regulatory domain (phosphotyrosines 1146, 1150, 1151) important for receptor kinase activation and the recruitment of downstream signaling protein. The LAR Tyrosine Phosphatase Assay Kit is a colorimetric, non-radioactive assay designed in a 96-well microplate format. It may be used to screen inhibitors and modulators of LAR phosphatase activity, and perform other experiments on LAR enzyme kinetics. The kit includes purified human LAR cytoplasmic domain (residues 1275-1613; MW=37 kDa), expressed in an E. coli expression system. The assay entails LAR dephosphorylation of a synthetic 12-mer peptide based on the regulatory autophosphorylation region of the IR β subunit cytoplasmic domain (residues 1142-1153) and phosphorylated at LAR’s preferred dephosphorylation site Tyr-1146. The phosphate released is quantified with the BIOMOL GREEN™ reagent. A tyrosine phosphatase inhibitor, RWJ-60475 is included as a control "hit" for inhibitor screening.
Product Specification
Long Term Storage: -80°C  
Kit/Set Contains: LAR Enzyme (human) (recombinant), Substrate (IR5 Insulin Receptor peptide), 2X Assay Buffer, BIOMOL GREEN™ Reagent, Phosphate Standard, RWJ-60475 Inhibitor, 1/2-volume Microplate.

-80°C