Anti-HMG-CoA reductase

货号： ABS229      产品名称： Anti-HMG-CoA reductase   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-HMG-CoA reductase
Recommended Replacement for: 07-572
Species Reactivity Key Applications Host Format Antibody Type
H  IP, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-HMG-CoA reductase Antibody
Replaces:
07-572
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Specificity:
This antibody recognizes HMG CoA reductase at the linker domain.
Molecular Weight:
~97 kDa observed
Epitope:
Linker domain
Immunogen:
KLH-conjugated linear peptide corresponding to the linker domain of human HMG CoA reductase.
Background Information:
The 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) enzyme is a ubiquitously expressed glycoprotein that is bound to the membrane of the endoplasmic reticulum (ER), but also projects an active C-terminal catalytic tail into the cytoplasm. It is the rate-limiting enzyme in the mevalonate pathway as it catalyzes the conversion of HMG CoA to mevalonate, which is a critical precursor protein in the synthesis of sterols such as cholesterols. In mammalian cells, HMG-CoA reductase is regulated by multiple mechanisms. It is downregulated by high levels of exogenous cholesterol bound to the low density lipoprotein. It is also regulated by sterol and nonsterol metabolites of mevalonate which may exert inhibitory effects at the transcriptional level. Previous studies have suggested that sterols may inhibit the sterol regulatory element-binding proteins (SREBPs) which function as transcription factors that enhance the expression of genes required for sterol biosynthesis. The end-stage degradation process may be mediated by the ubiquitin degradation system. The inhibition of HMG-CoA reductase has been widely studied for the treatment of cholesterol-related conditions.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Primate based on 100% sequence homology. Other homologies: Porcine (85% sequence homology).
Application Notes:
Immunoprecipitation Analysis: 10 µg/mL from a representative lot immunoprecipitated HMG CoA reductase from HepG2 cell lysate.
Control:
HepG2 cell lysate
Quality Assurance:
Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected HMG CoA reductase on 10 µg of HepG2 cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P04035
Entrez Gene Number:
NP_000850
Gene Symbol:
HMGCR
Alternate Names:
3-hydroxy-3-methylglutaryl-coenzyme A reductase
HMG-CoA reductase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.
CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
ENZYME REGULATION: Inhibited by statins, a class of hypoli 2-8℃