HEK-Blue CD40L cells
货号： hkb-cd40 产品名称： HEK-Blue CD40L cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ CD40L Cells
CD40L Sensor Cells
HEK-Blue™ CD40L cells can serve to measure the bioactivity of CD40L through the secretion of embryonic alkaline phosphatase (SEAP) upon NF-κB activation following CD40 stimulation. These cells were generated by stable transfection of HEK293 cells with the human CD40 gene and an NF-κB-inducible SEAP construct. Binding of CD40L to its receptor CD40 triggers a signaling cascade leading to the activation of NF-κB and the subsequent production of SEAP. CD40L-CD40 interaction can be monitored by assessing the levels of SEAP using QUANTI-Blue™. HEK293 cells express endogenously the receptors for the cytokines IL-1β and TNF-α which share a common signaling pathway with CD40L. Consequently, HEK-Blue™ CD40L cells also respond to IL-1β and TNF-α. IL-1β- and TNF-α-mediated SEAP production can be blocked using neutralizing antibodies, such as anti-hIL-1β-IgA and anti-hTNF-α-IgA respectively.
HEK-Blue™ CD40L cells are available in a kit containing anti-hCD40L-IgA.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Screen for molecules that interfere with CD40L-CD40 cross-talk
Detection range for human CD40L: 5 ng – 1 μg/ml
hkb-cd40-kit HEK-Blue CD40L kit 品牌 Invivogen
HEK-Blue CD40L kit
货号： hkb-cd40-kit 产品名称： HEK-Blue CD40L kit 品牌： Invivogen 规格： 1 kit 二周到货 生化试验
HEK-Blue™ CD40L Cells
CD40L Sensor Cells
HEK-Blue™ CD40L cells can serve to measure the bioactivity of CD40L through the secretion of embryonic alkaline phosphatase (SEAP) upon NF-κB activation following CD40 stimulation. These cells were generated by stable transfection of HEK293 cells with the human CD40 gene and an NF-κB-inducible SEAP construct. Binding of CD40L to its receptor CD40 triggers a signaling cascade leading to the activation of NF-κB and the subsequent production of SEAP. CD40L-CD40 interaction can be monitored by assessing the levels of SEAP using QUANTI-Blue™. HEK293 cells express endogenously the receptors for the cytokines IL-1β and TNF-α which share a common signaling pathway with CD40L. Consequently, HEK-Blue™ CD40L cells also respond to IL-1β and TNF-α. IL-1β- and TNF-α-mediated SEAP production can be blocked using neutralizing antibodies, such as anti-hIL-1β-IgA and anti-hTNF-α-IgA respectively.
HEK-Blue™ CD40L cells are available in a kit containing anti-hCD40L-IgA.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Screen for molecules that interfere with CD40L-CD40 cross-talk
Detection range for human CD40L: 5 ng – 1 μg/ml
hkb-hmdcd HEK-Blue hMD2-CD14 cells 品牌 Invivogen
HEK-Blue hMD2-CD14 cells
货号： hkb-hmdcd 产品名称： HEK-Blue hMD2-CD14 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hMD2-CD14
HEK-Blue™ MD2-CD14 cells were obtained by co-transfection of the human MD-2 and CD14 genes and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP reporter gene is under the control of the IL-12 p40 promoter fused to five NF-κB and AP-1 binding sites.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ MD2-CD14 cells is HEK-Blue™ Null2 cells.
Contents update notification
August, 5th 2013 – Please note cells now come with one pouch of HEK-Blue™ Detection for analysis purposes.
Specifications
Documents
Contents
Specifications
Antibiotic resistance: hygromycin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
hkb-hnod1 HEK-Blue hNOD1 cells 品牌 Invivogen
HEK-Blue hNOD1 cells
货号： hkb-hnod1 产品名称： HEK-Blue hNOD1 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hNOD1
HEK-Blue™-hNOD1 cells were obtained by co-transfection of the human NOD1 gene and an optimized secreted embryonic alkaline phosphatase (SEAP) reporter gene into HEK293 cells. The SEAP reporter gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1 binding sites. Stimulation with a NOD1 ligand activates NF-κB and AP-1 which induce the production of SEAP. Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hNOD1 cells is HEK-Blue™ Null1 cells.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
hkb-hnod2 HEK-Blue hNOD2 cells 品牌 Invivogen
HEK-Blue hNOD2 cells
货号： hkb-hnod2 产品名称： HEK-Blue hNOD2 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hNOD2
HEK-Blue™-hNOD2 cells were obtained by co-transfection of the human NOD2 gene and an optimized secreted embryonic alkaline phosphatase (SEAP) reporter gene into HEK293 cells.
The SEAP reporter gene is placed under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1 binding sites. Stimulation with a NOD2 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hNOD2 cells is HEK-Blue™ Null2 cells.
Contents update notification
August, 5th 2013 – Please note cells now come with one pouch of HEK-Blue™ Detection for analysis purposes.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
hkb-htlr2 HEK-Blue hTLR2 cells 品牌 Invivogen
HEK-Blue hTLR2 cells
货号： hkb-htlr2 产品名称： HEK-Blue hTLR2 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hTLR2
HEK-Blue™-hTLR2 cells were obtained by co-transfection of the human TLR2 and SEAP (secreted embryonic alkaline phosphatase) genes into HEK293 cells.
The SEAP reporter gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Additionally, the CD14 co-receptor gene was transfected into these cells to enhance the TLR2 response. Stimulation with a TLR2 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR2 cells is HEK-Blue™ Null1 cells.
Contents update notification
August, 5th 2013 – Please note cells now come with one pouch of HEK-Blue™ Detection for analysis purposes.
Antibiotic resistance: blasticidin, hygromycin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
hkb-htlr3 HEK-Blue hTLR3 cells 品牌 Invivogen
HEK-Blue hTLR3 cells
货号： hkb-htlr3 产品名称： HEK-Blue hTLR3 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hTLR3
HEK-Blue™-hTLR3 cells were obtained by co-transfection of the human TLR3 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR3 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR3 cells is HEK-Blue™ Null1 cells.
Contents update notification
August, 5th 2013 – Please note cells now come with one pouch of HEK-Blue™ Detection for analysis purposes.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
hkb-htlr4 HEK-Blue hTLR4 cells 品牌 Invivogen
HEK-Blue hTLR4 cells
货号： hkb-htlr4 产品名称： HEK-Blue hTLR4 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hTLR4
HEK-Blue™-hTLR4 cells were obtained by co-transfection of the human TLR4, MD-2 and CD14 co-receptor genes, and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP reporter gene is placed under the control of an IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR4 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR4 cells is HEK-Blue™ Null2 cells.
Contents update notification
August, 5th 2013 – Please note cells now come with one pouch of HEK-Blue™ Detection for analysis purposes.
Antibiotic resistance: blasticidin, hygromycin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
hkb-htlr5 HEK-Blue hTLR5 cells 品牌 Invivogen
HEK-Blue hTLR5 cells
货号： hkb-htlr5 产品名称： HEK-Blue hTLR5 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hTLR5
HEK-Blue™-hTLR5 cells were obtained by co-transfection of the human TLR5 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR5 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR5 cells is HEK-Blue™ Null1 cells.
Contents update notification
August, 5th 2013 – Please note cells now come with one pouch of HEK-Blue™ Detection for analysis purposes.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
hkb-htlr7 HEK-Blue hTLR7 cells 品牌 Invivogen
HEK-Blue hTLR7 cells
货号： hkb-htlr7 产品名称： HEK-Blue hTLR7 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hTLR7
HEK-Blue™-hTLR7 cells were obtained by co-transfection of the human TLR7 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP reporter gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR7 ligand activates NF-κB and AP-1 which induces the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR7 cells is HEK-Blue™ Null1-k cells.
Contents update notification
August, 5th 2013 – Please note cells now come with one pouch of HEK-Blue™ Detection for analysis purposes.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
hkb-htlr8 HEK-Blue hTLR8 cells 品牌 Invivogen
HEK-Blue hTLR8 cells
货号： hkb-htlr8 产品名称： HEK-Blue hTLR8 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hTLR8
HEK-Blue™-hTLR8 cells were obtained by co-transfection of the human TLR8 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR8 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR8 cells is HEK-Blue™ Null1 cells.
Contents update notification
August, 5th 2013 – Please note cells now come with one pouch of HEK-Blue™ Detection for analysis purposes.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
hkb-htlr9 HEK-Blue hTLR9 cells 品牌 Invivogen
HEK-Blue hTLR9 cells
货号： hkb-htlr9 产品名称： HEK-Blue hTLR9 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hTLR9
HEK-Blue™-hTLR9 cells were obtained by co-transfection of the human TLR9 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR9 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR9 cells is HEK-Blue™ Null1 cells.
Contents update notification
August, 5th 2013 – Please note cells now come with one pouch of HEK-Blue™ Detection for analysis purposes.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
hkb-ifnab HEK-Blue IFN-α/β cells 品牌 Invivogen
HEK-Blue IFN-α/β cells
货号： hkb-ifnab 产品名称： HEK-Blue IFN-α/β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ IFN-α/β Cells
Human Type I IFN Sensor Cells
HEK-Blue™ IFN-α/β cells allow the detection of bioactive human type I IFNs by monitoring the activation of the ISGF3 pathway. These cells were generated by stable transfection of HEK293 cells with the human STAT2 and IRF9 genes to obtain a fully active type I IFN signaling pathway. The other genes of the pathway (IFNAR1, IFNAR2, JAK1, TyK2 and STAT1) are naturally expressed in sufficient amounts. The cells were further transfected with a SEAP reporter gene under the control of the IFN-α/β inducible ISG54 promoter. Stimulation of HEK-Blue™ IFN-α/β cells with human IFN-α or IFN-β activates the JAK/STAT/ISGF3 pathway and subsequently induces the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™. Stimulation of HEK-Blue™ IFN-α/β cells with recombinant human IFN-α can be blocked by anti-hIFN-α-IgA, a neutralizing monoclonal antibody.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human type I interferons
• Detection range for human IFN-α: 1 – 104 IU/ml
• Detection range for human IFN-β: 10 – 104 IU/ml
hkb-il18 HEK-Blue IL-18/1L-1β cells 品牌 Invivogen
HEK-Blue IL-18/1L-1β cells
货号： hkb-il18 产品名称： HEK-Blue IL-18/1L-1β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ IL-18/IL-1β Cells
IL-18/IL-1β Sensor Cells
HEK-Blue™ IL-18/IL-1β cells are designed to detect bioactive IL-18 and IL-1β by monitoring the activation of the NF-κB and AP-1 pathways. They were generated by stable transfection of HEK-Blue™ IL-1β cells with the gene encoding the IL-18 receptor accessory protein (IL-18RAP). They express a NF-κB/AP-1 inducible SEAP reporter gene.
HEK-Blue™ IL-18/IL-1β cells are useful to monitor IL-18 and IL-1β in inflammasome activation studies. These cells detect the presence of IL-18 and IL-1β in samples which triggers the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™. Stimulation of HEK-Blue™ IL-18/IL-1β cells with recombinant human IL-1β or IL-18 can be blocked by the neutralizing antibodies anti-hIL-1β-IgA and anti-hIL-18-IgA, respectively. These antibodies allow to determine the respective contribution of each cytokine in inflammasome studies.
HEK-Blue™ IL-18/IL-1β cells are available in a kit containing anti-hIL-1β-IgA and anti-hIL-18-IgA.
Antibiotic resistance: blasticidin, hygromycin B, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human IL-18 and human/murine IL-1β (α)
• Detection range for human IL-18: 0.5 – 100 ng/ml
• Detection range for human IL-1β: 0.5 – 100 ng/ml
hkb-il18-kit HEK-Blue IL-18/IL-1β kit 品牌 Invivogen
HEK-Blue IL-18/IL-1β kit
货号： hkb-il18-kit 产品名称： HEK-Blue IL-18/IL-1β kit 品牌： Invivogen 规格： 1 kit 二周到货 生化试验
HEK-Blue™ IL-18/IL-1β Cells
IL-18/IL-1β Sensor Cells
HEK-Blue™ IL-18/IL-1β cells are designed to detect bioactive IL-18 and IL-1β by monitoring the activation of the NF-κB and AP-1 pathways. They were generated by stable transfection of HEK-Blue™ IL-1β cells with the gene encoding the IL-18 receptor accessory protein (IL-18RAP). They express a NF-κB/AP-1 inducible SEAP reporter gene.
HEK-Blue™ IL-18/IL-1β cells are useful to monitor IL-18 and IL-1β in inflammasome activation studies. These cells detect the presence of IL-18 and IL-1β in samples which triggers the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™. Stimulation of HEK-Blue™ IL-18/IL-1β cells with recombinant human IL-1β or IL-18 can be blocked by the neutralizing antibodies anti-hIL-1β-IgA and anti-hIL-18-IgA, respectively. These antibodies allow to determine the respective contribution of each cytokine in inflammasome studies.
HEK-Blue™ IL-18/IL-1β cells are available in a kit containing anti-hIL-1β-IgA and anti-hIL-18-IgA.
Antibiotic resistance: blasticidin, hygromycin B, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human IL-18 and human/murine IL-1β (α)
• Detection range for human IL-18: 0.5 – 100 ng/ml
• Detection range for human IL-1β: 0.5 – 100 ng/ml
hkb-il1b HEK-Blue IL-1β cells 品牌 Invivogen
HEK-Blue IL-1β cells
货号： hkb-il1b 产品名称： HEK-Blue IL-1β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ IL-1β Cells
IL-1β Sensor cells
HEK-Blue™ IL-1β cells allow to detect bioactive IL-1β by monitoring the activation of the NF-κB and AP-1 pathways. They derive from HEK-Blue™ TNF-α/IL-1β cells in which the TNF-α response has been blocked. Therefore, HEK-Blue™ IL-1β cells respond specifically to IL-1β. They express a NF-κB/AP-1-inducible SEAP reporter gene. Binding of IL-1β to its receptor IL-1R on the surface of HEK-Blue™ IL-1β cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP.
HEK-Blue™ IL-1β cells are useful to monitor IL-1β in inflammasome activation studies. These cells detect the presence of IL-1β in samples which triggers the production of SEAP. Levels of SEAP can be easily monitored using QUANTI-Blue™. Stimulation of HEK-Blue™ IL-1β cells with recombinant human IL-1β can be blocked by the neutralizing monoclonal anti-hIL-1β-IgA antibody.
HEK-Blue™ IL-1β cells are also available in a kit containing anti-hIL-1β-IgA.
Antibiotic resistance: hygromycin B, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-Glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56°C), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human and murine IL-1β (α)
• Detection range for human IL-1β: 100 pg – 100 ng/ml
• Detection range for murine IL-1β: 10 ng – 1 µg/ml
hkb-il1b-kit HEK-Blue IL-1β kit 品牌 Invivogen
HEK-Blue IL-1β kit
货号： hkb-il1b-kit 产品名称： HEK-Blue IL-1β kit 品牌： Invivogen 规格： 1 kit 二周到货 生化试验
HEK-Blue™ IL-1β Cells
IL-1β Sensor cells
HEK-Blue™ IL-1β cells allow to detect bioactive IL-1β by monitoring the activation of the NF-κB and AP-1 pathways. They derive from HEK-Blue™ TNF-α/IL-1β cells in which the TNF-α response has been blocked. Therefore, HEK-Blue™ IL-1β cells respond specifically to IL-1β. They express a NF-κB/AP-1-inducible SEAP reporter gene. Binding of IL-1β to its receptor IL-1R on the surface of HEK-Blue™ IL-1β cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP.
HEK-Blue™ IL-1β cells are useful to monitor IL-1β in inflammasome activation studies. These cells detect the presence of IL-1β in samples which triggers the production of SEAP. Levels of SEAP can be easily monitored using QUANTI-Blue™. Stimulation of HEK-Blue™ IL-1β cells with recombinant human IL-1β can be blocked by the neutralizing monoclonal anti-hIL-1β-IgA antibody.
HEK-Blue™ IL-1β cells are also available in a kit containing anti-hIL-1β-IgA.
Antibiotic resistance: hygromycin B, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-Glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56°C), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human and murine IL-1β (α)
• Detection range for human IL-1β: 100 pg – 100 ng/ml
• Detection range for murine IL-1β: 10 ng – 1 µg/ml
hkb-il33 HEK-Blue IL-33/IL-1β cells 品牌 Invivogen
HEK-Blue IL-33/IL-1β cells
货号： hkb-il33 产品名称： HEK-Blue IL-33/IL-1β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ IL-33/IL-1β Cells
IL-33/IL-1β Reporter Cells
HEK-Blue™ IL-33/IL-1β cells are designed to detect bioactive IL-33 and IL-1β (and IL-1α) by monitoring the activation of the NF-κB and AP-1 pathways. They were generated by stable transfection of HEK-Blue™ IL-1β cells with the IL1RL1 gene. They express a SEAP repor ter gene under the control of the IFN-β minimal promoter fused to five NF-κB and five AP-1 binding sites. Stimulation of HEK-Blue™ IL-33/IL-1β cells with human IL-33 or IL-1β, but not with IL-18 nor TNF-α, activates the NF-κB and AP-1 pathways triggering the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™. Stimulation of HEK-Blue™ IL-33/IL-1β cells with recombinant human IL-1β can be blocked by using the neutralizing antibody anti-hIL-1β-IgA.
Antibiotic resistance: blasticidin, hygromycin B, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human IL-33 and human/murine IL-1β (α)
• Detection range for human IL-33: 0.5 – 100 ng/ml
• Detection range for human IL-1β: 0.5 – 100 ng/ml
hkb-il6 HEK-Blue IL-6 cells 品牌 Invivogen
HEK-Blue IL-6 cells
货号： hkb-il6 产品名称： HEK-Blue IL-6 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ IL-6 Cells
IL-6 Reporter Cells
HEK-Blue™ IL-6 cells allow the detection of bioactive IL-6 by monitoring the activation of the STAT-3 pathway. These cells were generated by stable transfection of HEK293 cells with the human IL-6R gene and a STAT3-inducible SEAP reporter gene. Upon IL-6 stimulation, HEK-Blue™ IL-6 cells trigger the activation of STAT3 and the subsequent secretion of SEAP. SEAP can be readily monitored using QUANTI-Blue™. Stimulation of HEK-Blue™ IL-6 cells with recombinant human IL-6 can be blocked by hIL-6-IgA, a neutralizing monoclonal antibody of the IgA isotype.
HEK-Blue™ IL-6 cells are also available in a kit containing hIL-6-IgA.
Antibiotic resistance: Hygromycin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human IL-6
Detection range for human IL-6: 0.5 – 50 ng/ml
hkb-il6-kit HEK-Blue IL-6 kit 品牌 Invivogen
HEK-Blue IL-6 kit
货号： hkb-il6-kit 产品名称： HEK-Blue IL-6 kit 品牌： Invivogen 规格： 1 kit 二周到货 生化试验
HEK-Blue™ IL-6 Cells
IL-6 Reporter Cells
HEK-Blue™ IL-6 cells allow the detection of bioactive IL-6 by monitoring the activation of the STAT-3 pathway. These cells were generated by stable transfection of HEK293 cells with the human IL-6R gene and a STAT3-inducible SEAP reporter gene. Upon IL-6 stimulation, HEK-Blue™ IL-6 cells trigger the activation of STAT3 and the subsequent secretion of SEAP. SEAP can be readily monitored using QUANTI-Blue™. Stimulation of HEK-Blue™ IL-6 cells with recombinant human IL-6 can be blocked by hIL-6-IgA, a neutralizing monoclonal antibody of the IgA isotype.
HEK-Blue™ IL-6 cells are also available in a kit containing hIL-6-IgA.
Antibiotic resistance: Hygromycin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human IL-6
Detection range for human IL-6: 0.5 – 50 ng/