HEK-Blue IFN-α/β cells
货号： hkb-ifnab 产品名称： HEK-Blue IFN-α/β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ IFN-α/β Cells
Human Type I IFN Sensor Cells
HEK-Blue™ IFN-α/β cells allow the detection of bioactive human type I IFNs by monitoring the activation of the ISGF3 pathway. These cells were generated by stable transfection of HEK293 cells with the human STAT2 and IRF9 genes to obtain a fully active type I IFN signaling pathway. The other genes of the pathway (IFNAR1, IFNAR2, JAK1, TyK2 and STAT1) are naturally expressed in sufficient amounts. The cells were further transfected with a SEAP reporter gene under the control of the IFN-α/β inducible ISG54 promoter. Stimulation of HEK-Blue™ IFN-α/β cells with human IFN-α or IFN-β activates the JAK/STAT/ISGF3 pathway and subsequently induces the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™. Stimulation of HEK-Blue™ IFN-α/β cells with recombinant human IFN-α can be blocked by anti-hIFN-α-IgA, a neutralizing monoclonal antibody.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human type I interferons
• Detection range for human IFN-α: 1 – 104 IU/ml
• Detection range for human IFN-β: 10 – 104 IU/ml
bb-ifnab B16-Blue IFNα/β cells 品牌 Invivogen
B16-Blue IFNα/β cells
货号： bb-ifnab 产品名称： B16-Blue IFNα/β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
B16-Blue™ ISG CellsNEW!
Interferon Regulatory Factor-Inducible SEAP Reporter B16 Melanocytes
B16-Blue™ ISG cells allow the detection of bioactive murine type I and II IFNs by monitoring the activation of the JAK/STAT pathway.
B16-Blue™ ISG cells can also be used study the activation of the TBK1/IRF3 pathway by cytosolic DNA, dsRNA or CDNs.
B16-Blue™ ISG cells were derived from the murine B16 F1 melanoma cell line. They express the secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
Stimulation of B16- Blue™ ISG cells with IFNs, CDNs, such as cGAMP, or type I IFN inducers, such as transfected poly(dA:dT), triggers the activation of the I-ISG54 promoter and the production of SEAP.
Levels of SEAP in the supernatant can be easily determined using QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP, by reading the OD at 620-655 nm.
B16-Blue™ ISG cells are resistant to Zeocin™.
Antibiotic resistance: Zeocin™
Growth Medium: RPMI, 10% (v/v) fetal bovine serum (FBS), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™, 2 mM L-glutamine
Murine IFN Detection range :
• mIFN-α: 10e2 – 10e4 IU/ml
• mIFN-β: 10e2 – 10e4 IU/ml
• mIFN-γ: 0.1 ng – 1 µg/ml
Quality control
Reporter activity is validated by stimulating the cells with murine IFN-α (mIFN-α), mIFN-β, mIFN-γ, and IRF3 activators, such as poly(dA:dT)/LyoVec™, c-di-GMP and cGAMP.
The cells are guaranteed mycoplasma-free.