标签归档：cav

ST1701-100UG   ANTI-CAV3 MOUSE MAB (1G11)   品牌 Merck

发表于由

ANTI-CAV3 MOUSE MAB (1G11)

货号： ST1701-100UG      产品名称： ANTI-CAV3 MOUSE MAB (1G11)   品牌： Merck 规格： *** 3周到货 生化实验

Anti-CAV3 Mouse mAb (1G11)
Anti-Caveolin3 
Recognizes the CAV3 protein.
产品信息
Host  Mouse
Isotype  IgG2b
Immunogen  A recombinant polypeptide corresponding to amino acids of 1-84 human CAV3, expressed as a GST fusion protein
Clone  1G11
Form  liquid
Formulation  In PBS, pH 7.2.
Species reactivity  human
Preservative  None
Applications 
Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Avoid freeze/thaw  是的
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Regulatory Review

-20°C

MABN464   Anti-Cav3.1 Ca2+ channel, clone N178A/9   品牌 Millipore

发表于由

Anti-Cav3.1 Ca2+ channel, clone N178A/9

货号： MABN464      产品名称： Anti-Cav3.1 Ca2+ channel, clone N178A/9   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Cav3.1 Ca2+ channel, clone N178A/9
Species Reactivity Key Applications Host Format Antibody Type
M, R  WB, IHC Mouse Purified Monoclonal Antibody
Description:
Anti-Cav3.1 Ca2+ channel Antibody, clone N178A/9
Promotional Text:
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100% Performance Guaranteed
Molecular Weight:
~230 kDa observed. An uncharacterized band at ~53 kDa may be observed in some tissue lysates.
Immunogen:
Recombinant protein corresponding to mouse Cav3.1.
Clone:
N178A/9
Isotype:
IgG1κ
Background Information:
Voltage-dependent T-type calcium channel subunit alpha-1G or Voltage-gated calcium channel subunit alpha Cav3.1, like other members of the voltage-sensitive calcium channel (VSCC) family, mediate the entry of calcium ions into excitable cells. These channels regulate and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1G gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by mibefradil. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and also support calcium signaling in secretory cells and vascular smooth muscle.
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Species Reactivity:
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Mouse and Rat. Other homologies: Human (80% sequence homology).
Application Notes:
Immunohistochemistry Analysis: 1 µg/mL from a representative lot detected Cav3.1 Ca2+ channel in rat thalamus tissue.
Immunohistochemistry Analysis: A representative lot detected Cav3.1 Ca2+ in mouse thalamus, cortex, and cerebellum tissue.
Immunohistochemistry Analysis: A representative lot detected Cav3.1 Ca2+ in rat hippocampus tissue.
Western Blot Analysis: A representative lot detected Cav3.1 Ca2+ in rat brain membrane tissue lysate.
Western Blot Analysis: A representative lot detected Cav3.1 Ca2+ in rat brain membrane tissue lysate and in WT mouse brain membrane tissue lysate, but demonstrates a loss of signal in Cav3.1 Ca2+1 knockout mouse brain membrane tissue lysate.
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Control:
Mouse cerebellum tissue
Quality Assurance:
Evaluated by Western Blot in mouse cerebellum tissue.
Western Blot Analysis: 1 µg/mL of this antibody from a representative lot detected Cav3.1 Ca2+ channel in 10 µg/mL of mouse cerebellum tissue.
Purification Method:
Protein G purified
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9WUT2
Entrez Gene Number:
NP_001106284
Gene Symbol:
Cacna1g
Alternate Names:
Voltage-gated calcium channel, alpha-1-G subunit
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Product Name:
Anti-Cav3.1 Ca2+ channel, clone N178A/9
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type: 2-8℃

AB10515   ANTI-Cav1.2 alpha1c   品牌 Millipore

发表于由

ANTI-Cav1.2 alpha1c

货号： AB10515      产品名称： ANTI-Cav1.2 alpha1c   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Cav1.2 alpha1c
Recommended Replacement for: AB9902
Species Reactivity Key Applications Host Format Antibody Type
M, H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Cav1.2 alpha1c Antibody
Replaces:
AB9902
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
The antibody reacts with the 170-240 kDa Cav1.2 alpha1c protein.
Molecular Weight:
170-240 kDa
Epitope:
N-terminus
Immunogen:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminus region of human Cav1.2 alpha1c.
Background Information:
This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
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Species Reactivity:
Mouse
Human
Species Reactivity Note:
Reacts with mouse. Other species have not been tested. 100% homology to human, 93.3% homology to (14/15aa) mouse, rat, opposum
Control:
Western Blot : Mouse Pancreas lysate.
Quality Assurance:
Western Blot Analysis: 1:1000 dilution of this antibody detected Cav1.2 alpha1c on mouse pancreas lysate.
Purification Method:
Antigen affinity purified
Presentation:
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Storage Conditions:
Store at 2-8°C for 1 year from date of receipt.
UniProt Number:
Q01815
Entrez Gene Number:
Q13936
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the ‘high-voltage activated’ (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart.
SUBUNIT STRUCTURE: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel act 2-8℃

AB5491-200UL   CAV3.1 CALCIUM CHANNEL, RBX-200UL   品牌 Millipore

发表于由

CAV3.1 CALCIUM CHANNEL, RBX-200UL

货号： AB5491-200UL      产品名称： CAV3.1 CALCIUM CHANNEL, RBX-200UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Calcium Channel Cav3.1 Antibody
Species Reactivity Key Applications Host Format Antibody Type
R  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Calcium Channel Cav3.1 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Cav3.1. The epitope specific for Cav3.1 is not present in any other known proteins.
SPECIES REACTIVITIES:
The immunogen sequence is conserved in human (20/22). Reactivity with other species has not yet been tested.
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Immunogen:
Highly purified peptide corresponding to residues 1-22 of rat Cav3.1 (Accession O54898).
Species Reactivity:
Rat
Application Notes:
Western blotting: 1:200 using ECL on rat brain membranes. See suggested protocol.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
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Control:
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation:
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Storage Conditions:
Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
O43497
Entrez Gene Number:
NM_018896.3
NM_198376.1
NM_198377.1
NM_198378.1
NM_198379.1
NM_198380.1
NM_198382.1
NM_198383.1
NM_198384.1
NM_198385.1
NM_198386.1
NM_198387.1
NM_198388.1
NM_198396.1
NM_198397.1
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Gene Symbol:
CACNA1G
Ca(V)T.1
KIAA1123
Cav3.1
Cav3.1c
NBR13
MGC117234
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Alternate Names:
T-type Alpha 1G
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Voltage-activated calcium channels can be distinguished based on their voltage-dependence, deactivation, and single-channel conductance. See MIM 601011. Low-voltage-activated calcium channels are referred to as ‘T’ type because their currents are both transient, owing to fast inactivation, and tiny, owing to small conductance. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing.[supplied by OMIM]
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UniProt Summary:
FUNCTION: SwissProt: O43497 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1G gives rise to T-type calcium currents. T -20℃

AB9020-50UL   CAV3.3, RBX-50UL   品牌 Millipore

发表于由

CAV3.3, RBX-50UL

货号： AB9020-50UL      产品名称： CAV3.3, RBX-50UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Calcium Channel Cav3.3 Antibody
Species Reactivity Key Applications Host Format Antibody Type
R  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Calcium Channel Cav3.3 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
Cav3.3
Immunogen:
Synthetic peptide from rat Cav3.3 (Accession Q9Z0Y8).The immunogen sequence is 14/15 residues identical in human.
Species Reactivity:
Rat
Application Notes:
Western blot: 1:200 using ECL on rat brain membrane proteins.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
SUGGESTED WESTERN BLOT PROTOCOL
1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.
2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.
3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)
4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).
5. Destain with deionized water.
6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).
7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.
8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.
9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.
10. Wash 4 x 10 min. with PBS-0.1% tween 20.
11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600).
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Control:
Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation:
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Storage Conditions:
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Q9P0X4
Entrez Gene Number:
NM_001003406.1
NM_021096.3
Gene Symbol:
CACNA1I
KIAA1120
v.3).
Cav3.3
Ca
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Alternate Names:
alpha 1I
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro di -20℃