Fluor-de-Lys deacetylase substrate

货号： BML-KI104-0050      产品名称： Fluor-de-Lys deacetylase substrate   品牌： Biomol 规格： 50 ul 3周到货 生化实验

Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).
Product Specification
MW:298.3
Quantity:2.5 µmol (50 µl)
Formulation:Supplied as a 50 mM solution (50 µl) in dimethylsulfoxide (DMSO).
Purity:95% (HPLC)
Long Term Storage:-80°C
 

-80°C

Fluor-de-Lys developer Iconcentrate

货号： BML-KI105-0300      产品名称： Fluor-de-Lys developer Iconcentrate   品牌： Biomol 规格： 300 ul 3周到货 生化实验

Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).
Product Specification
MW:298.3
Quantity:2.5 µmol (50 µl)
Formulation:Supplied as a 50 mM solution (50 µl) in dimethylsulfoxide (DMSO).
Purity:95% (HPLC)
Long Term Storage:-80°C
 

-80°C

Fluor-de-Lys deacetylated standard

货号： BML-KI142-0030      产品名称： Fluor-de-Lys deacetylated standard   品牌： Biomol 规格： 30 ul 3周到货 生化实验

Product Specification
Long Term Storage:-20°C

-20°C

Fluor-de-Lys-H4-AcK16 deacetylase substrate

货号： BML-KI174-0005      产品名称： Fluor-de-Lys-H4-AcK16 deacetylase substrate   品牌： Biomol 规格： 0.5 umol 3周到货 生化实验

The deacetylated state of lysine-16 of histone H4 (H4-K16) is thought to be a critical factor in determining regions of chromatin silencing. Conversely, acetylation of lysine-16 can be associated with increased transcription, such as that due to dosage compensation from the X-chromosome of male Drosophila. The acetylated H4 lysine-16 is preferentially deacetylated by yeast Sir2 in vitro and in vivo. Fluor de Lys®-H4-AcK16 is a fluorogenic, acetylated peptide substrate suitable for various lysine deacetylases, including the HDAC activities in HeLa nuclear extract, HDAC8, SIRT1 (human Sirtuin 1) and SIRT2 (human Sirtuin 2). It is based on residues from histone H4 (12-16, Lys-Gly-Gly-Ala-Lys(Ac)) and was the preferred substrate for both SIRT1 and SIRT2 within a group patterned on acetylation sites in histone H3 and histone H4 N-terminal tails. Fluor de Lys®-H4-AcK16 is deacetylated by SIRT1 (BML-SE239) at a rate of more than 6-fold and by SIRT2 (BML-SE251) at a rate of more than 40-fold those achieved with the acetylated lysine substrate, Fluor de Lys® (BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD ). Must be used in conjunction with Fluor de Lys® Developer (BML-KI105). Sufficient for 100-200 assays (50-100 µM substrate).
Product Specification
MW:298.4
Formulation:Supplied as a 5 mM solution (100 µl) in HDAC Assay Buffer.
Purity:95%
Long Term Storage:-80°C

-80°C

Fluor-de-Lys developer II

货号： BML-KI176-1250      产品名称： Fluor-de-Lys developer II   品牌： Biomol 规格： 1.25 mL 3周到货 生化实验

Product Specification
Quantity:NOTE: This product is supplied packaged in 5 tubes (250 µl per tube) for a total volume of 1.25 ml. This packaging is used for optimal stability of the developer.
Concentration:5x concentrate (1.25 ml). After dilution (1x), sufficient for 100 assays (50 µl reaction volume).
Long Term Storage:-80°C

-80°C

Fluor-de-Lys-SIRT1 deacetylase substrate

货号： BML-KI177-0005      产品名称： Fluor-de-Lys-SIRT1 deacetylase substrate   品牌： Biomol 规格： 0.5 umol 3周到货 生化实验

Product Specification
Quantity:0.5 µmol (100 µl)
Formulation:Supplied as a 5 mM solution in HDAC Assay Buffer.
Purity:≥95% (HPLC)
Long Term Storage:-80°C

-80°C

Fluor-de-Lys-HDAC8 deacetylase substrate

货号： BML-KI178-0005      产品名称： Fluor-de-Lys-HDAC8 deacetylase substrate   品牌： Biomol 规格： 0.5 umol 3周到货 生化实验

Fluor de Lys®-HDAC8 is a fluorogenic, diacetylated peptide substrate for HDAC8 (histone deacetylase-8). Based on residues 379-382 of p53 (Arg-His-Lys(Ac)-Lys(Ac)), a site of regulatory acetylation by the p300 and CBP acetyltransferases (lysines 381, 382), it was the best for HDAC8 from among a panel of substrates patterned on p53, histone H3 and histone H4 acetylation sites. Fluor de Lys®-HDAC8 is deacetylated by HDAC8 at a rate of more than 10-fold that of the acetylated lysine substrate, Fluor de Lys® (BML-KI104; substrates both at 100 µM). Although named because of HDAC8’s preference for it, it is also an excellent substrate for SIRT1 (BMl-SE239; 5x the rate of BML-KI104 at 25 µM, 0.5 mM NAD+), SIRT2 and HeLa Nuclear Extract (BML-KI140; 3x KI-104 rate, 25 µM). Must be used in conjunction with Fluor de Lys® Developer II (BML-KI176). Sufficient for 100-200 assays of human recombinant HDAC8 (BML-SE145; 1 U/well, 50-100 µM substrate).
Product Specification
Formulation:Supplied as a 5 mM solution (100 µl) in HDAC Assay Buffer.
Purity:95%
Long Term Storage:-80°C

-80°C

Fluor-de-Lys-SIRT2 deacetylase substrate

货号： BML-KI179-0005      产品名称： Fluor-de-Lys-SIRT2 deacetylase substrate   品牌： Biomol 规格： 0.5 umol 3周到货 生化实验

Fluor de Lys®-SIRT2 is a fluorogenic peptide substrate for SIRT2 (human Sirtuin 2). Based on residues 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), a site of regulatory acetylation by the PCAF acetyltransferase (lysine 320), it was the best for SIRT2 from among a panel of substrates patterned on p53, Histone H3, and Histone H4 acetylation sites. Fluor de Lys®-SIRT2 is deacetylated by SIRT2 at a rate of more than 50-fold that of the acetylated lysine substrate, Fluor de Lys® (BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD+). Must be used in conjunction with Fluor de Lys® Developer II (BML-KI176). Sufficient for 100 assays of human recombinant SIRT2 (BML-SE251; 2U/well, 100 µM substrate).
Product Specification
Formulation:Supplied as a 5mM solution (100 µl) in HDAC Assay Buffer.
Purity:≥95% (HPLC)
Long Term Storage:-80°C

-80°C