标签归档：phospho

07-2119   Anti-phospho-RalA (Ser194)   品牌 Millipore

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Anti-phospho-RalA (Ser194)

货号： 07-2119      产品名称： Anti-phospho-RalA (Ser194)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-RalA (Ser194)
Description:
Anti-phospho-RalA Antibody (Ser194)
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Specificity:
This antibody recognizes RalA phosphorylated at Ser194.
Molecular Weight:
~25 kDa observed. Uncharacterized bands may be observed at ~20 kDa, 100 kDa, and 130-170 kDa in some cell lysates.
Immunogen:
KLH-conjugated linear peptide corresponding to human RalA phosphorylated at Ser194.
Background Information:
RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes. RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarized exocytosis in epithelial cells.
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Species Reactivity:
Human
Bovine
Rat
Xenopus
Zebrafish
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Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Bovine, Rat, Xenopus, and Zebrafish based on 100% sequence homology.
Application Notes:
Western Blot Analysis: A representative lot detected phospho-RalA (Ser194) in certain primary and metastatic cell lysates, and in certain unmatched tumor tissue lysate samples.
Control:
PANC10.05 cell lysate
Quality Assurance:
Evaluated by Western Blot in PANC10.05 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected phospho-RalA in PANC10.05 cell lysate. Peptide inhibition was observed with the addition of phospho peptides but not with the addition of non-phospho peptides.
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Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P11233
Entrez Gene Number:
NP_005393
Gene Symbol:
RALA
Ral
Alternate Names:
Ras-related protein Ral-A
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Peptide Inhibition Assay
Product Name:
Anti-phospho-RalA (Ser194)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit

2°C – 8°C

07-2126   Anti-phospho-FOXO1 (Thr24)   品牌 Millipore

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Anti-phospho-FOXO1 (Thr24)

货号： 07-2126      产品名称： Anti-phospho-FOXO1 (Thr24)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-FOXO1 Antibody (Thr24)
Description:
Anti-phospho-FOXO1 Antibody (Thr24)
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Specificity:
This antibody recognizes FOXO1 phosphorylated at Thr24.
Molecular Weight:
~78 kDa observed.
Calculated molecular weight is 70 kDa. FOXO1 has been shown to migrate to ~78 kDa in western blots (Kamei, Y., et al. (2004). JBC. 279(39):41114-41123).
Epitope:
Phosphorylated Thr24
Immunogen:
KLH-conjugated linear peptide corresponding to human FOXO1 phosphorylated at Thr24.
Modifications:
Phosphorylation
Background Information:
Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions and cell proliferation. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. Increased cell proliferation occurs when FOXO1 is inactivated when phosphorylated by Akt at Thr24.
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Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
HEK293 cell lysate untreated and BMP2-treated
Quality Assurance:
Evaluated by Western Blot in HEK293 cell lysate untreated and BMP2-treated.
Western Blot Analysis: 2 µg/mL of this antibody detected phospho-FOXO1 (Thr24) in HEK293 cell lysate untreated and BMP2-treated.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q12778
Entrez Gene Number:
NP_002006
Gene Symbol:
FOXO1, FOXO1A, FKHR
Alternate Names:
Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
Function: Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
Subunit structure: Interacts with LRPPRC. Interacts with SIRT1 and this interaction requires the presence of KRIT1.
Subcellular location: Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus.
Tissue specificity: Ubiquitous.
Induction: Expression is regulated by KRIT1.
Post-translational modification: Phosphorylated by AKT1; insulin-induced. IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and lea 2°C – 8°C

07-2177   Anti-phospho-PKD2 (Ser197/Ser200)   品牌 Millipore

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Anti-phospho-PKD2 (Ser197/Ser200)

货号： 07-2177      产品名称： Anti-phospho-PKD2 (Ser197/Ser200)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-PKD2 Antibody (Ser197/Ser200)
Description:
Anti-phospho-PKD2 Antibody (Ser197/Ser200)
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Specificity:
This antibody recognizes PKD2 phosphorylated at Ser197/Ser200.
Molecular Weight:
~110 kDa observed. An uncharacterized band appears at ~150 kDa in some lysates.
Epitope:
Phosphorylated Ser197/Ser200
Immunogen:
KLH-conjugated linear peptide corresponding to human PKD2 phosphorylated at Ser197/Ser200.
Modifications:
Phosphorylation
Phosphorylation
Background Information:
As members of the calcium-/calmodulin-depedent protein kinase superfamily, there are three known Protein Kinase D (PKD) isoforms, PKD1, PKD2, and PKD3. These serine/threonine kinases are involved in protein-protein and protein-carbohydrate adhesion and are also thought to be ion channel regulators. They are prominent downstream targets of protein kinase C and phospholipase D in various biological systems including mouse. PKDs are activated by various stimuli, including phorbol esters, G-protein-coupled receptors, reactive oxygen species, and diacylglycerol. PKDs regulate protein transport in the trans-Golgi network and are thought to play a role in muscle differentiation, cell motility, migration, and invasion. These isoforms are known to interact with each other. PKD2 is shown to be activated by phorbol esters, both in vivo and in vitro, and also by gastrin via the cholescystokinin/CCK(B) receptor in stably-transfected AGS-B cells. Furthermore, PKD2 is found to be active during the early phase of differentation of C2C12 cells into skeletal muscle cells. It is also reported to enhance TCR-induced NFAT activity in Jurkat T cells.
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Species Reactivity:
Mouse
Human
Rat
Species Reactivity Note:
Demonstrated to react with Mouse.
Predicted to react with Human and Rat based on 100% sequence homology.
Application Notes:
Evaluated by Western Blot in MEF cell lysates untreated and treated with PDBu, Rapamycin, and GO6983.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected PKD2 in 10 µg of MEF cell lysates untreated and treated with PDBu, Rapamycin, and GO6983.
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Control:
MEF cell lysates untreated and PBDu-treated
Quality Assurance:
Evaluated by Western Blot in MEF cell lysates untreated and PBDu-treated.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected PKD2 in 10 µg of MEF cell lysates untreated and PBDu-treated.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q13563
Entrez Gene Number:
NP_000288
Gene Symbol:
PC2
PKD4
Pc-2
APKD2
TRPP2
MGC138466
MGC138468
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Alternate Names:
Autosomal dominant polycystic kidney disease type II protein
Polycystic kidney disease 2 protein
Polycystwin
R48321
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a member of the polycystin protein family. The encoded p 2°C – 8°C

07-2273   Anti-phospho-SPAK (Ser373) / phosph   品牌 Millipore

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Anti-phospho-SPAK (Ser373) / phosph

货号： 07-2273      产品名称： Anti-phospho-SPAK (Ser373) / phosph   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)
Description:
Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)
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Specificity:
This antibody recognizes SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.
Molecular Weight:
~60 kDa observed
Epitope:
SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.
Immunogen:
KLH-conjugated linear peptide corresponding to human SPAK and OSR1 phosphorylated at Ser375 and Ser325, respectively.
Modifications:
Phosphorylation
Background Information:
As members of the (sterile 20)-like (STE20) kinase family, the oxidative-stress-responsive kinase 1 (OSR1) and the SPS1-related proline/alanine-rich kinase (SPAK) serve as key enzymes in a signaling cascade, which mediate the activity of Na+/K+/2Cl- cotransporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which are 79% identical to each other at the sequence level, and are also highly conserved in orthologues of these enzymes in Caenorhabditis elegans, Drosophila, and Xenopus. The CCT domain reflects no similarity to sequences found in other proteins. This domain is known to recognize and interact with a unique peptide (Arg-Phe-Xaa-Val) motif, which is present in OSRT1- and SPAK-activating kinases (with-no-lysine-kinase 1 (WNK1 and WNK4) and also in STE20 kinase family substrates (NKCC1 and NKCC2). It is reported that phosphorylation of a Ser/Thr residue preceding the (Arg-Phe-Xaa-Val) motif results in steric clash, and subsequent dissociation from the CCT domain. Furthermore, patients suffering from Gordon syndrome carry mutated WNK1 and WNK4 genes.
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Species Reactivity:
Human
Mouse
Rat
Canine
Species Reactivity Note:
Demonstrated to react with Human and Mouse.
Predicted to react with Rat and Canine based on 100% sequence homology.
Control:
Hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.
Quality Assurance:
Evaluated by Western Blot in hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.
Western Blot Anlaysis: 0.5 µg/mL of this antibody detected SPAK / OSR1 in 10 µg hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.
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Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9UEW8
Entrez Gene Number:
NP_037365
Gene Symbol:
STK39
SPAK
Alternate Names:
DCHT
Serine/threonine-protein kinase 39
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that th 2°C – 8°C

04-339   anti-phospho-EGF Receptor (Tyr1068)   品牌 Millipore

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anti-phospho-EGF Receptor (Tyr1068)

货号： 04-339      产品名称： anti-phospho-EGF Receptor (Tyr1068)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-EGFR (Tyr1068), clone EP774Y, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB, ICC, IH(P) Rabbit Unpurified Monoclonal Antibody
Description:
Anti-phospho-EGFR (Tyr1068) Antibody, clone EP774Y, rabbit monoclonal
Promotional Text:
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Specificity:
This antibody recognizes EGFR phosphorylated at (Tyr1068).
Molecular Weight:
~170 kDa observed
Epitope:
Phosphorylated (Tyr1068)
Immunogen:
Synthetic peptide corresponding to human EGFR phosphorylated at (Tyr1068).
Clone:
EP774Y
Isotype:
IgG
Background Information:
Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, when activated by epidermal growth factor (EGF), affects cell growth and differentiation in normal and cancer cells (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). EGFR overexpression is exhibited in various cancers, such as glioma, colorectal carcinoma, breast carcinoma, and head and neck carcinoma (4). The phosphorylation level of EGFR is considered one of the important predictors of clinical outcome in non-small cell lung cancer therapy (5).
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Demonstrated to react with Human and Mouse.
Application Notes:
Immunohistochemistry Analysis: A 1:250-500 dilution from a representative lot was used in breast adenocarcinoma tissue.
Immunocytochemistry Analysis: A 1:250-500 dilution from a representative lot was used in IC.
Control:
A431 cell lysate
Quality Assurance:
Evaluated by Western Blot in A431 cell lysate untreated and EGF treated.
WesternBlot Analysis: A 1:5,000 dilution of this antibody detected EGFR phosphorylated at (Tyr1068) in A431 cell lysate untreated and EGF treated.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing glycerol, BSA, and sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P00533
Entrez Gene Number:
P00533
Gene Symbol:
ERBB1
EC 2.7.10.1
ERBB
HER1
OTTHUMP00000159661
OTTHUMP00000159662
OTTHUMP00000159663
OTTHUMP00000209210
OTTHUMP00000209211
PIG61
mENA
EC 2.7.10
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Alternate Names:
epidermal growth factor receptor
Proto-oncogene c-ErbB-1
Receptor tyrosine-protein kinase erbB-1
epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)
oncogene homolog)
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
cell growth inhibiting protein 40
cell proliferation-inducing protein 61
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying -20°C

04-340   anti-phospho-EGF Receptor (Tyr1086)   品牌 Millipore

发表于由

anti-phospho-EGF Receptor (Tyr1086)

货号： 04-340      产品名称： anti-phospho-EGF Receptor (Tyr1086)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-EGFR (Tyr1086), clone Y39, Rabbit Monoclonal
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Species Reactivity Key Applications Host Format Antibody Type
H  ICC, IP, WB Rabbit Unpurified Monoclonal Antibody
Description:
Anti-phospho-EGFR (Tyr1086) Antibody, clone Y39, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes EGFR phosphorylated at (Tyr1086).
Molecular Weight:
~170 kDa observed
Epitope:
Phosphorylated (Tyr1068)
Immunogen:
Synthetic peptide corresponding to human EGFR phosphorylated at (Tyr1086).
Clone:
Y39
Isotype:
IgG
Background Information:
Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). Once activated, EGFR phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. Another autophosphorylation site, Tyr 1086, is situated within the cytoplasmic domain, and is phosphorylated to a higher extent by c-Src than by EGFR (4).
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot was used in A431 cells.
Immunoprecipitation Analysis: A 1:150 dilution from a representative lot was used in IP.
Control:
A431 cell lysate
Quality Assurance:
Evaluated by Western Blot in A431 cell lysate untreated and EGF treated.
WesternBlot Analysis: A 1:10,000 dilution of this antibody detected EGFR phosphorylated at (Tyr1086) in A431 cell lysate untreated and EGF treated.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing glycerol, BSA, and sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P00533
Entrez Gene Number:
P00533
Gene Symbol:
ERBB1
EC 2.7.10.1
ERBB
HER1
OTTHUMP00000159661
OTTHUMP00000159662
OTTHUMP00000159663
OTTHUMP00000209210
OTTHUMP00000209211
PIG61
mENA
EC 2.7.10
View All »
Alternate Names:
epidermal growth factor receptor
Proto-oncogene c-ErbB-1
Receptor tyrosine-protein kinase erbB-1
epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)
oncogene homolog)
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
cell growth inhibiting protein 40
cell proliferation-inducing protein 61
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are -20°C

05-483NA-K   Anti-phospho-EGFR Receptor (Y1173),   品牌 Millipore

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Anti-phospho-EGFR Receptor (Y1173),

货号： 05-483NA-K      产品名称： Anti-phospho-EGFR Receptor (Y1173),   品牌： Millipore 规格： MG 三周到货 生化实验

Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2
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REFERENCES
Hyaluronan and the interaction between CD44 and epidermal growth factor receptor in oncogenic signaling and chemotherapy resistance in head and neck cancer.
Steven J Wang, Lilly Y W Bourguignon, Steven J Wang, Lilly Y W Bourguignon (2006) Archives of otolaryngology–head neck surgery.132:771-8
The antiepidermal growth factor receptor agent gefitinib (ZD1839/Iressa) improves antihormone response and prevents development of resistance in breast cancer in vitro.
J M W Gee, M E Harper, I R Hutcheson, T A Madden, D Barrow, J M Knowlden, R A McClelland, N Jordan, A E Wakeling, R I Nicholson (2003) Endocrinology.144:5105-17
Enhanced estrogen receptor (ER) alpha, ERBB2, and MAPK signal transduction pathways operate during the adaptation of MCF-7 cells to long term estrogen deprivation.
Martin, Lesley-Ann, et al. (2003) J. Biol. Chem., 278: 30458-68 (2003)
ACh and adenosine activate PI3-kinase in rabbit hearts through transactivation of receptor tyrosine kinases.
Thomas Krieg, Qining Qin, Elizabeth C McIntosh, Michael V Cohen, James M Downey (2002) American journal of physiology. Heart and circulatory physiology.283:H2322-30
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Species Reactivity Key Applications Host Format Antibody Type
M, R, H, Ca  ELISA, ICC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the motif NAE[pY]LRV corresponding to the major auto-phosphorylation site of the human EGFR, Mr 175 kDa. Tyr1173 corresponds to Tyr1197 in the human (isoforms a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins. This antibody does not interact with the non-phosphorylated EGFR or with other unrelated phosphotyrosine proteins.
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Molecular Weight:
175 kDa
Immunogen:
KLH-conjugated, synthetic peptide containing the Y1173 motif (…NAE[pY1173]LRV…) of the human EGFR.
Modifications:
Phosphorylation
Clone:
9H2
Isotype:
IgG1κ
Background Information:
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. There is also evidence to suggest that clusters of activated EGFRs form, although it remains unclear whether this clustering is important for activation itself or occurs subsequent to activation of individual dimers.
EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine (Y) residues in the C-terminal domain of EGFR occurs. These are Y992, Y1045, Y1068, Y1148 and Y1173. This autophosphorylation elicits downstream activation and signaling by several other prot -20℃

05-636X-KL   Anti-phospho-Histone H2A.X (Ser139), clo   品牌 Millipore

发表于由

Anti-phospho-Histone H2A.X (Ser139), clo

货号： 05-636X-KL      产品名称： Anti-phospho-Histone H2A.X (Ser139), clo   品牌： Millipore 规格： MG 三周到货 生化实验

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301
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REFERENCES
PARP inhibition sensitizes to low dose-rate radiation TMPRSS2-ERG fusion gene-expressing and PTEN-deficient prostate cancer cells.
Chatterjee, Payel, et al. (2013) PLoS ONE, 8: e60408 (2013)
Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression.
Culver-Cochran, Ashley E and Chadwick, Brian P (2013) BMC Genomics, 14: 740 (2013)
Homologous recombination DNA repair genes play a critical role in reprogramming to a pluripotent state.
González, Federico, et al. (2013) Cell Rep, 3: 651-60 (2013)
Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination.
Tang, Jiangbo, et al. (2013) Nat. Struct. Mol. Biol., 20: 317-25 (2013)
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Species Reactivity Key Applications Host Format Antibody Type
Vrt  ICC, IF, WB, ChIP Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Histone H2A.X phosphorylated at Ser139.
Molecular Weight:
17 kDa
Immunogen:
peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X
Modifications:
Phosphorylation
Clone:
JBW301
Isotype:
IgG1
Background Information:
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ‘beads on a string’ structure.
Species Reactivity:
Vertebrates
Application Notes:
Additional Referenced Applications:
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
Control:
UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue
Quality Assurance:
Immunoblot Analysis: 0.05-1 μg/ml of this antibody detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.
View All »
Purification Method:
Protein G purfied
Presentation:
Immunoaffinity Purified immunoglobulin in 0.1M Tris-Glycine,pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.
Storage Conditions:
Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
UniProt Number:
P16104
Entrez Gene Number:
NP002096
Gene Symbol:
H2AFX
H2AX
H2a/x
H2A/X
H2A.X
View All »
Alternate Names:
H2AXS139P
Histone H2A.X (phospho S139)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunocytochemistry
Immunofluorescence
Western Blotting
Chromatin Immunoprecipitation (ChIP)
Entrez Gene Summary:< 2-8℃

05-746R-KL   Anti-phospho-Histone H3 (Thr 3), cl   品牌 Millipore

发表于由

Anti-phospho-Histone H3 (Thr 3), cl

货号： 05-746R-KL      产品名称： Anti-phospho-Histone H3 (Thr 3), cl   品牌： Millipore 规格： ML 三周到货 生化实验

Anti-phospho-Histone H3 (Thr3), clone JY325
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PRODUCT FAMILY INFORMATION
Histone H3 Antibodies, Proteins and Kits
Millipore’s Histone H3 antibodies demonstrate specificity against histone H3. See below for acetyl-, methyl-, phospho- histone H3 Antibodies and Proteins, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H  WB, PIA Rabbit Purified Monoclonal Antibody
Description:
Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal
Promotional Text:
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100% Performance Guaranteed
Specificity:
This antibody recognizes Histone H3 phosphorylated at Thr3.
Molecular Weight:
~17 kDa observed
Epitope:
Phosphorylated Thr3
Immunogen:
KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Thr3.
Modifications:
Phosphorylation
Clone:
JY325
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Consistent with this role, Histone H3 phosphorylated at threonine 3 has been shown to be recognized by the BIR domain Survivin, a member of the inhibitor of apoptosis (IAP) family.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Peptide Block Analysis: This antibody peptide blocked on colcemid treated HeLa cell extracts.
Control:
Colcemid treated HeLa acid extract and recombinant Histone H3 negative control.
Quality Assurance:
Evaluated by Western Blot using colcemid treated HeLa acid extract and recombinant Histone H3 negative control.
Western Blot Analysis: 1:8,000 dilution of this antibody detected Histone H3 in 10 µg of colcemid treated HeLa acid extract.
Purification Method:
Protein A
Presentation:
Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide and 40% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P68431
Entrez Gene Number:
NP_066298
Gene Symbol:
HIST1H3A
H3FA
HIST1H3B
H3FL
HIST1H3C
H3FC
HIST1H3D
H3FB
HIST1H3E
H3FD
HIST1H3F
H3FI
HIST1H3G
H3FH
HIST1H3H
H3FK
H -20℃

05-922R   Anti-phospho-Estrogen Receptor alpha(Ser   品牌 Millipore

发表于由

Anti-phospho-Estrogen Receptor alpha(Ser

货号： 05-922R      产品名称： Anti-phospho-Estrogen Receptor alpha(Ser   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-Estrogen Receptor alpha (Ser305), clone 124.9.4
Species Reactivity Key Applications Host Format Antibody Type
H  WB, IHC Rabbit Purified Monoclonal Antibody
Description:
Anti-phospho-Estrogen Receptor alpha (Ser305) Antibody, clone 124.9.4, rabbit monoclonal
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Specificity:
Thia antibody recognizes Estrogen Receptor alpha phosphorylated at Ser305.
Molecular Weight:
~66 kDa observed
Epitope:
Phosphorylated Ser305
Immunogen:
KLH-conjugated linear peptide corresponding to human Estrogen Receptor alpha phosphorylated at Ser305.
Clone:
124.9.4
Isotype:
IgG
Background Information:
Estrogen Receptor α (ERα) is a nuclear hormone receptor. This steroid hormone receptor is involved in the regulation of eukaryotic gene expression and affects cellular proliferation and differentiation in target tissues. ERα is a ligand-activated transcription factor and upon being bound by a ligand such as estrogen, a conformational change occurs that allows dimerization and binding to an estrogen response element. When bound to DNA, Estrogen Receptor α can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Two ERs are known, ERα, classically known as ER, and ERβ. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ appears to be distinct from those regulating ERα.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Estrogen Receptor alpha in ductal carcinoma.
Control:
PKA phosphorylated and unphosphorylated recombinant ERα protein.
Quality Assurance:
Evaluated by Western Blot in PKA phosphorylated and unphosphorylated recombinant ERα protein.
Western Blot Analysis: 1µg/mL dilution of this antibody detected Estrogen Receptor alpha on 10 µg of PKA phosphorylated and unphosphorylated recombinant ERα protein.
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Purification Method:
Protein A purified
Presentation:
Purified rabbit monoclonal IgG in buffer containing PBS with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P03372
Entrez Gene Number:
NP_000116
Gene Symbol:
ESR1
ESR
NR3A1
Alternate Names:
Estrogen receptor
ER-alpha
Estradiol receptor
Nuclear receptor subfamily 3 group A member 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunohistochemistry
Entrez Gene Summary:
Summary: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcript 2°C – 8°C

06-1370   Anti-phospho-Histone H1 (Thr165)   品牌 Millipore

发表于由

Anti-phospho-Histone H1 (Thr165)

货号： 06-1370      产品名称： Anti-phospho-Histone H1 (Thr165)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-Histone H1 (Thr165)
Species Reactivity Key Applications Host Format Antibody Type
H  WB, DB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-Histone H1 (Thr165) Antibody
Promotional Text:
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Specificity:
This antibody recognizes Histone H1 phosphorylated at Thr165.
Molecular Weight:
~33 kDa observed. A cross-reacting band is observed at ~110 kDa.
Epitope:
Phosphorylated Thr165
Immunogen:
KLH-conjugated linear peptide corresponding to human Histone H1 phosphorylated at Thr165.
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Broad species crossreactivity due to conserved homology.
Application Notes:
Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-phospho-Histone H1 (Thr165) (1:5,000 dilution).
Control:
Untreated and colcemid treated HeLa acid extract, and recombinant Histone H1 protein
Quality Assurance:
Evaluated by Western Blot in untreated and colcemid treated HeLa acid extract, and recombinant Histone H1 protein.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 on 10 µg of untreated and colcemid treated HeLa acid extract, and recombinant Histone H1 protein.
View All »
Purification Method:
Antigen Affinity purified
Presentation:
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P16403
Entrez Gene Number:
NP_005310
Gene Symbol:
HIST1H1C
H1F2
Alternate Names:
Histone H1.2
Histone H1d
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Dot Blot
Product Name:
Anti-phospho-Histone H1 (Thr165)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Use Anti-phospho-Histone H1 (Thr165) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB to detect phospho-Histone H1 (Thr165) also known as Histone H1.2, Histone H1d.

2°C – 8°C

06-1423   Anti-phospho-Lck (Tyr505)   品牌 Millipore

发表于由

Anti-phospho-Lck (Tyr505)

货号： 06-1423      产品名称： Anti-phospho-Lck (Tyr505)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-Lck (Tyr505)
Species Reactivity Key Applications Host Format Antibody Type
H, R, M, Po, Sh, B  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-Lck (Tyr505) Antibody
Promotional Text:
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Specificity:
This antibody recognizes Lck phosphorylated at Tyr505.
Molecular Weight:
~56 kDa observed
Epitope:
Phosphorylated Tyr505
Immunogen:
KLH-conjugated linear peptide corresponding to human Lck phosphorylated at Tyr505.
Modifications:
Phosphorylation
Background Information:
Anti-Phospho-Lck (Tyr505) detects endogenous levels of Lck only when phosphorylated at Tyr505. Lck is a member of the Src family of tyrosine kinases and is most commonly found in T cells. It associates with the cytoplasmic tails of the CD4 on T helper cells and CD8 co-receptors on cytotoxic T cells. The tyrosine phosphorylation cascade initiated by Lck leads to the intracellular mobilization of a calcium (Ca2+) ions and activation of important signaling cascades within the lymphocyte including the Ras-MEK-ERK pathway, which goes on to activate certain transcription factors such as NFAT, NF-κB, and AP-1. These transcription factors regulate the production of gene products, such as Interleukin-2.
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Species Reactivity:
Human
Rat
Mouse
Pig
Sheep
Bovine
View All »
Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Rat, Mouse, Porcine, Sheep, and Bovine based on 100% sequence homology. Other homologies: Xenopus, Zebrafish, and Chicken (90% sequence homology).
Control:
Hydrogen peroxide untreated and treated Jurkat cell lysate
Quality Assurance:
Evaluated by Western Blot in hydrogen peroxide untreated and treated Jurkat cell lysate.
Western Blot Analysis: A 1:20,000 dilution of this antibody detected Lck on 10 µg of hydrogen peroxide untreated and treated Jurkat cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P06239
Entrez Gene Number:
NP_005347
Gene Symbol:
LCK
lck
Alternate Names:
Tyrosine-protein kinase Lck
Leukocyte C-terminal Src kinase
LSK
Lymphocyte cell-specific protein-tyrosine kinase
Protein YT16
Proto-oncogene Lck
T cell-specific protein-tyrosine kinase
p56-LCK
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other 2°C – 8°C

06-1433   Anti-phospho-FGFR-1 (Tyr653/Tyr654)   品牌 Millipore

发表于由

Anti-phospho-FGFR-1 (Tyr653/Tyr654)

货号： 06-1433      产品名称： Anti-phospho-FGFR-1 (Tyr653/Tyr654)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-FGFR-1 (Tyr653/Tyr654)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Ch, B, Xn  WB, Cell Function Assay Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody
Promotional Text:
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100% Performance Guaranteed
Specificity:
This antibody recognizes FGFR-1 phosphorylated at Tyr653/Tyr654.
Molecular Weight:
~145 kDa observed.
FGFR-1 has 19 isoforms and eight potential glycosylation sites that increase the expected size to 120 kDa for the immature form and 145 kDa for the mature form of the receptor (Hisaoka, K., et al. (2011). JBC. 286(24):21118-21128).
Epitope:
Phosphorylated Tyr653/Tyr654
Immunogen:
KLH-conjugated linear peptide corresponding to human FGFR-1 phosphorylated at Tyr653/Tyr654.
Modifications:
Phosphorylation
Phosphorylation
Background Information:
FGFR-1 (fibroblast growth factor receptor 1) is a member of the basic fibroblast growth factor family of proteins and is thought to be important in the induction and propagation of benign prostatic hyperplasia. A shortage of FGFR-1 causes death post-implantation and has been linked to neurofascin and neurite outgrowth. FGFR-1 expression has recently been found to be increased in patients with renal cell carcinoma and FGFR-1 may also have a role to play in early limb formation.
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Species Reactivity:
Human
Mouse
Rat
Chicken
Bovine
Xenopus
View All »
Species Reactivity Note:
Demonstrated to react with Human.
Predicted to react with Mouse, Rat, Chicken, Bovine, Xenopus, and Zebra Fish based on 100% sequence homology.
Application Notes:
Peptide Inhibition Assay Analysis: 0.2 µg/mL from a representative lot peptide blocked in HEK293T cells transfected with FGFR-1.
Control:
Untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR-1
Quality Assurance:
Evaluated by Western Blot in untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.
Western Blot Analysis: 0.2 µg/mL of this antibody detected FGFR-1 in 10 µg of untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.
View All »
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P11362
Entrez Gene Number:
NP_075598
Gene Symbol:
FGFR1
FGFBR
FLG
FLT2
Alternate Names:
Basic fibroblast growth factor receptor 1
FGFR-1
bFGF-R-1
Fms-like tyrosine kinase 2
FLT-2
Proto-oncogene c-Fgr
CD331
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Cell Function Assay
Entrez Gene Summary:
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distributi 2°C – 8°C

07-018-I   Anti-phospho-p70 S6 Kinase (Thr389)   品牌 Millipore

发表于由

Anti-phospho-p70 S6 Kinase (Thr389)

货号： 07-018-I      产品名称： Anti-phospho-p70 S6 Kinase (Thr389)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-p70 S6 Kinase (Thr389)
Species Reactivity Key Applications Host Format Antibody Type
H  WB, PIA Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-p70 S6 Kinase (Thr389) Antibody
Promotional Text:
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100% Performance Guaranteed
Specificity:
This antibody recognizes the AGC-kinase C-terminal of Ribosomal protein S6 kinase beta-1 phosphorylated at Thr389.
Molecular Weight:
~70 kDa observed
Epitope:
AGC-kinase C-terminal
Immunogen:
KLH-conjugated linear peptide corresponding to the AGC-kinase C-terminal of human Ribosomal protein S6 kinase beta-1
phosphorylated at Thr389.
Background Information:
The p70 S6 kinase is a ubiquitous cytoplasmic protein that is activated in response to cytokines, and growth factors including the epidermal growth factor and fibroblast growth factor. It lies downstream of the mTOR/PI3K pathway and is phosphorylated on multiple residues: Thr389, Thr229, Thr421, Ser371, Ser404, Ser411, Ser418, and Ser424. Thr389, Ser371, and Ser404 are located in the linker domain; whereas Thr421, Ser411, Ser418, Ser424 are located in the autoinhibitory domain, and Thr229 is found in the catalytic domain. These sites may be phosphorylated in a hierarchical sequence leading to the activation of the p70 S6 kinase. The p70 S6 kinase phosphorylates the S6 protein on the 40S ribosomal protein (rpS6), and plays a role in multiple processes including regulation of mRNA molecules with 5’-terminal oligopyrimidine tracts, protein synthesis, cell growth, proliferation, and cell survival. The activity of this kinase may be important in diabetes, cancer, and aging.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Mouse, Rat, Chicken, Zebra Fish, and Bovine based on 100% sequence homology. Other homologies: ribosomal protein S6 kinase beta-2 (91% sequence homology).
Control:
MCF7 cell lysate
Quality Assurance:
Evaluated by Western Blot (Peptide Inhibition) Analysis in MCF7 cell lysate and MCF7 cell lysate with non-phospho or phospho peptide.
Western Blot (Peptide Inhibition) Analysis: 1.0 µg/mL of this antibody detected p70 S6 Kinase phosphorylated at Thr389 in 10 µg of MCF7 cell lysate and MCF7 cell lysate incubated with a non-phospho peptide.
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Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P23443
Entrez Gene Number:
NP_003152
Gene Symbol:
RPS6KB1
STK14A
Alternate Names:
Ribosomal protein S6 kinase
S6K-beta-1
S6K1
p70S6K1
p70 S6K1
p70-S6K1
p70 S6KA
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Peptide Inhibition Assay
Product Name:
Anti-phospho-p70 S6 Kinase (Thr389)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purif 2°C – 8°C

07-1347   Anti-phospho-STAT3 (Tyr68)   品牌 Millipore

发表于由

Anti-phospho-STAT3 (Tyr68)

货号： 07-1347      产品名称： Anti-phospho-STAT3 (Tyr68)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-STAT3 (Tyr68)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, B, Po, Xn, Ch  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-STAT3 (Tyr68) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes STAT3 phosphorylated at Tyr68.
Molecular Weight:
~75 kDa observed
Epitope:
Phosphorylated Tyr68
Immunogen:
KLH-conjugated linear peptide corresponding to human STAT3 phosphorylated at Tyr68.
Modifications:
Phosphorylation
Background Information:
This protein is a member of the family of STAT proteins (Signal Transduction and Activators of Transcription). These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. The Stat3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1. Stat3 is not activated by IFN beta. One active Stat3 translocates to the nucleus and binds to DNA as either a homodimer or as a heterodimer with Stat1. Transcription factors associated with Stat3 include cJun and cyclic AMP responsive enhancer binding protein (CREB). Stat3 interacts with the APRE (acute phase response element) and the SIE (c-sis inducible element).
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Species Reactivity:
Human
Mouse
Rat
Bovine
Pig
Xenopus
Chicken
View All »
Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Porcine, Xenopus, Zebra Fish and Chicken based on 100% sequence homology.
Control:
PMA treated and untreated MOLT4 cell lysates
Quality Assurance:
Evaluated by Western Blot in PMA treated and untreated MOLT4 cell lysates.
Western Blot Analysis: 5 µg/mL of this antibody detected STAT3 on 10 µg of PMA treated and untreated MOLT4 cell lysates.
Purification Method:
Antigen affinity
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P40763
Entrez Gene Number:
NP_003141
Gene Symbol:
STAT3
APRF
Alternate Names:
Signal transducer and activator of transcription 3
Acute-phase response factor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
Summary: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, I 2°C – 8°C

07-1507   Anti-phospho-Mypt1 (Ser507)   品牌 Millipore

发表于由

Anti-phospho-Mypt1 (Ser507)

货号： 07-1507      产品名称： Anti-phospho-Mypt1 (Ser507)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-Mypt1 (Ser507)
Species Reactivity Key Applications Host Format Antibody Type
M, H, B, Ch  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-Mypt1 (Ser507) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes Mypt1 phosphorylated at (Ser507).
Molecular Weight:
~120 kDa observed.
Uniprot gives a calculated molecular weight of 115 kDa, which can be observed at higher molecular weights due to hyperphosphorylation. Uncharacterized bands appear at ~30 kDa and ~130 kDa in some lysates.
Epitope:
Phosphorylated (Ser507)
Immunogen:
KLH-conjugated linear peptide corresponding to human Mypt1 phosphorylated at (Ser507).
Modifications:
Phosphorylation
Background Information:
Myosin phosphatase targeting subunit 1 (MYPT1) is a regulatory targeting subunit that is thought to be responsible for targeting the holoenzyme to the proper positions in muscle and moving PP1 to myosin. MYPT1 is the major subunit involved in myosin light chain phosphatase (MLCP). It is also essential for vasodilation caused by nitric oxide (NO). A decrease in MYPT1 has been shown to play a role in increased tolerance of NO in pulmonary arteries.
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Species Reactivity:
Mouse
Human
Bovine
Chicken
Species Reactivity Note:
Demonstrated to react with Mouse.
Predicted to react with Human, Bovine, and Chicken based on 100% sequence homology. Other homologies: Rat (89% sequence homology).
Control:
Untreated and PMA-treated NIH/3T3 cell lysates
Quality Assurance:
Evaluated by Western Blot in untreated and PMA-treated NIH/3T3 cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected Mypt1 in 10 µg of untreated and PMA-treated NIH/3T3 cell lysates.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O14974
Entrez Gene Number:
NP_002471
Gene Symbol:
PPP1R12A
MBS
MYPT1
Alternate Names:
Protein phosphatase 1 regulatory subunit 12A
Myosin phosphatase-targeting subunit 1
Myosin phosphatase target subunit 1
Protein phosphatase myosin-binding subunit
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and conse 2-8℃

07-1517   Anti-phospho-IRS-2 (Ser388)   品牌 Millipore

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Anti-phospho-IRS-2 (Ser388)

货号： 07-1517      产品名称： Anti-phospho-IRS-2 (Ser388)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-IRS-2 (Ser388)
Species Reactivity Key Applications Host Format Antibody Type
H  WB, PIA Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-IRS-2 (Ser388) Antibody
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Specificity:
This antibody recognizes IRS-2 phosphorylated at Ser388.
Molecular Weight:
~160 kDa observed. The calculated molecular weight is 137 kDa, however IRS-2 has been shown as a ~160 kDa band in western blots (Rother, K., et al. (1998). Journal of Biological Chemistry. 273(28):17491–17497.)
Epitope:
Phosphorylated Ser388
Immunogen:
KLH-conjugated linear peptide corresponding to human IRS-2 phosphorylated Ser388.
Background Information:
Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic beta cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Peptide Inhibition Assay Analysis: 10 µg from a representative lot blocked IRS-2 on insulin treated HEK293 cells.
Control:
Untreated and insulin treated HEK293 cell lysates
Quality Assurance:
Evaluated by Western Blot in untreated and insulin treated HEK293 cell lysates.
Western Blot Analysis: 5 µg/mL of this antibody detected IRS-2 on 10 µg of untreated and insulin treated HEK293 cell lysates.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9Y4H2
Entrez Gene Number:
NP_003740
Gene Symbol:
IRS2
Alternate Names:
Insulin receptor substrate 2
IRS-2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Peptide Inhibition Assay
Product Name:
Anti-phospho-IRS-2 (Ser388)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Anti-phospho-IRS-2 (Ser388) Antibody detects level of phospho-IRS-2 (Ser388) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay.

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