NEK6, Active

货号： 7716-5      产品名称： NEK6, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active NEK6
Kinase involved in cell cycle, checkpoint control and cancer
Product Overview
Alternate Names / Synonyms: neb6, neb-6, neb 6, Active NEK6, NEK6, human NEK6, active kinase, active kinases, NEK, recombinant NEK6, recombinant NEK, kinase activity assay, protein kinase
Gene Symbol: NEK6
Accession #: Q9HC98
Gene ID: 10783
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: 63.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 153 nmol phosphate incorporated into MBP per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: Nek6 is a serine/threonine kinase that is a member of the Nek family of protein kinases that share an amino-terminal catalytic domain related to NIMA (never in mitosis, gene A) family (1). Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. Nek6 is phosphorylated and activated during M phase of the cell cycle (2). Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis suggesting that Nek6 is required for metaphase-anaphase transition. Nercc1/Nek9 binds to Nek6 and is likely to be responsible for the activation of Nek6 during mitosis representing a new signaling pathway that regulates mitotic progression (3). Northern blot analysis shows that Nek6 transcript is ubiquitously expressed with the highest expression found in the heart and skeletal muscle. Nek6 effectively phosphorylates histones H1 and H3, but not casein suggesting that, unlike other mammalian NIMA-related kinases, Nek6 is a mitotic histone kinase which regulates chromatin condensation in mammalian cells. In addition, Nek6 phosphorylates p70 S6 kinase at Thr412 and other sites and activates the p70 S6 kinase in vitro and in vivo, in a manner synergistic with PDK1 (4). Kinase-inactive Nek6 interferes with insulin activation of p70 S6 kinase implicating Nek6 as a possible physiologic regulator of the p70 S6 kinase.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

NEK7, Active

货号： 7717-5      产品名称： NEK7, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active NEK7
Kinase involved in cell cycle, checkpoint control and cancer
Product Overview
Alternate Names / Synonyms: nek 7, nek-7, Active NEK7, NEK7, human NEK7, active kinase, active kinases, NEK, recombinant NE7, recombinant NEK, kinase activity assay, protein kinase
Gene Symbol: NEK7
Accession #: NM_133494
Gene ID: 140609
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: 63.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 221 nmol phosphate incorporated into β-casein per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: Nek7 is a member of the NIMA (never in mitosis, gene A) family of serine/threonine kinases. In contrast to the other documented NIMA-related kinases, Nek7 harbor its catalytic domain in the C-terminus of the protein. Immunofluorescence studies suggest that Nek7 is cytoplasmic and located on chromosome 1 (1). During early embryogenesis Nek7 is expressed in the site of decidual reaction while later in embryogenesis, it is almost exclusively restricted to the nervous system in the dorsal thalamus (2). The major protein kinase that is active on the p70 S6 kinase hydrophobic regulatory site (FXXFS/TF/Y) Thr412 was purified from rat liver and identified as Nek7 (3). Nek7 kinase activity is rapidly and efficiently increased by serum deprivation, and may be regulated in a cell cycle-dependent manner.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

NEK2, Active

货号： 7719-5      产品名称： NEK2, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active NEK2
Kinase involved in cell cycle, checkpoint control and cancer
Product Overview
Alternate Names / Synonyms: nek 2, nek-2, Active NEK2, NEK2, human NEK2, active kinase, active kinases, NEK, recombinant NEK2, recombinant NEK, kinase activity assay, protein kinase
Gene Symbol: NEK2
Accession #: P51955
Gene ID: 4751
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol).
Molecular Weight: 76.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 183 nmol phosphate incorporated into MBP per minute per mg protein at 300C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: Nek 2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulated progressively throughout S, reaching maximal levels in late G2 (1). These observations demonstrate that Nek2 resembles Aspergillus NIMA, not only in its catalytic domain, but also in its cell cycle-dependent expression. Recombinant Nek2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human Nek2 and fungal NIMA phosphorylate a similar, albeit not identical, set of proteins and synthetic peptides, and β-casein is a suitable substrate for assaying Nek2 in vitro (2). Nek2 is shown to be expressed most abundantly in the testis of the adult tissues examined (3). Its expression in the testis is restricted to the germ cells, with highest levels detected in spermatocytes at pachytene and diplotene stages. Immunohistochemical analysis revealed that Nek2 localized to nuclei, exhibiting a non-uniform distribution within the nucleus.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C