4011   PIPETTE,1ML,PW,PS,S,IND,100BAG 1ml移液管 灭菌 独立包装 PW(paper/platic wrap)   品牌 Corning

PIPETTE,1ML,PW,PS,S,IND,100BAG 1ml移液管 灭菌 独立包装 PW(paper/platic wrap)

货号: 4011      产品名称: PIPETTE,1ML,PW,PS,S,IND,100BAG 1ml移液管 灭菌 独立包装 PW(paper/platic wrap)   品牌: Corning 规格: 10包/箱 100个/包

BML-PW0150-0025   Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)   品牌 Biomol

Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)

货号: BML-PW0150-0025      产品名称: Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)   品牌: Biomol 规格: 25 ug 3周到货 生化实验

Product Specification
Clone:FK2
Host:Mouse
Isotype:IgG1
Immunogen:Crude preparation of polyubiquitinylated-lysozyme.
Application:ELISA
Immunohistochemistry
Western Blot (1:1’000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes mono- and polyubiquitinylated protein conjugates in a wide range of species. Does not cross-react with free ubiquitin.
Formulation:Liquid. In PBS.
Use/Stability:Antibody is supplied at a concentration of 1mg/mL. Store at 4ºC. Can be stored at -20ºC if required, but avoid multiple freeze/thawing. Vial contains purified HRP-linked immunoglobulin suspended in phosphate-buffered saline.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:+4°C
Background / Technical Information:FK2 antibody was conjugated to horseradish peroxidase (HRP) by a proprietary method. The HRP-linked FK2 monoclonal antibody (FK2H) gives enhanced western blotting detection of mono- and poly-ubiquitinylated protein conjugates and multi-ubiquitin chains compared to the unconjugated FK2 antibody (PW8810). For western blotting an initial antibody dilution of 1:1000 is recommended, followed by washing and signal detection using appropriate development reagents.
Note: Milk should NOT be used in blocking/antibody binding solutions. 1% BSA in PBS or TBS Tween should be used instead.

+4°C

BML-PW0150-0100   Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)   品牌 Biomol

Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)

货号: BML-PW0150-0100      产品名称: Mono- and polyubiquitinylated conjugates, mAb (FK2H) (HRP conjugate)   品牌: Biomol 规格: 100 ug 3周到货 生化实验

Product Specification
Clone:FK2
Host:Mouse
Isotype:IgG1
Immunogen:Crude preparation of polyubiquitinylated-lysozyme.
Application:ELISA
Immunohistochemistry
Western Blot (1:1’000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes mono- and polyubiquitinylated protein conjugates in a wide range of species. Does not cross-react with free ubiquitin.
Formulation:Liquid. In PBS.
Use/Stability:Antibody is supplied at a concentration of 1mg/mL. Store at 4ºC. Can be stored at -20ºC if required, but avoid multiple freeze/thawing. Vial contains purified HRP-linked immunoglobulin suspended in phosphate-buffered saline.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:+4°C
Background / Technical Information:FK2 antibody was conjugated to horseradish peroxidase (HRP) by a proprietary method. The HRP-linked FK2 monoclonal antibody (FK2H) gives enhanced western blotting detection of mono- and poly-ubiquitinylated protein conjugates and multi-ubiquitin chains compared to the unconjugated FK2 antibody (PW8810). For western blotting an initial antibody dilution of 1:1000 is recommended, followed by washing and signal detection using appropriate development reagents.
Note: Milk should NOT be used in blocking/antibody binding solutions. 1% BSA in PBS or TBS Tween should be used instead.

+4°C

BML-PW0325-0025   Sp100 (human), pAb   品牌 Biomol

Sp100 (human), pAb

货号: BML-PW0325-0025      产品名称: Sp100 (human), pAb   品牌: Biomol 规格: 25 ul 3周到货 生化实验

Product Specification
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 292-304 of human Sp100.
UniProt ID:P23497
Application:Immunocytochemistry
Western Blot
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human Sp100 and SUMO modified Sp100 species.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C

-20°C

BML-PW0325-0100   Sp100 (human), pAb   品牌 Biomol

Sp100 (human), pAb

货号: BML-PW0325-0100      产品名称: Sp100 (human), pAb   品牌: Biomol 规格: 100 ul 3周到货 生化实验

Product Specification
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 292-304 of human Sp100.
UniProt ID:P23497
Application:Immunocytochemistry
Western Blot
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human Sp100 and SUMO modified Sp100 species.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C

-20°C

BML-PW0330-0025   Sp100 (SUMO modified) (human), pAb   品牌 Biomol

Sp100 (SUMO modified) (human), pAb

货号: BML-PW0330-0025      产品名称: Sp100 (SUMO modified) (human), pAb   品牌: Biomol 规格: 25 ul 3周到货 生化实验

Product Specification
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 292-304 of human SP100, SUMO modified at Lys297.
UniProt ID:P23497
Application:Immunocytochemistry
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human SUMO-Sp100. Does not cross-react with unmodified Sp100 under native conditions.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C

-20°C

BML-PW0330-0100   Sp100 (SUMO modified) (human), pAb   品牌 Biomol

Sp100 (SUMO modified) (human), pAb

货号: BML-PW0330-0100      产品名称: Sp100 (SUMO modified) (human), pAb   品牌: Biomol 规格: 100 ul 3周到货 生化实验

Product Specification
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 292-304 of human SP100, SUMO modified at Lys297.
UniProt ID:P23497
Application:Immunocytochemistry
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human SUMO-Sp100. Does not cross-react with unmodified Sp100 under native conditions.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C

-20°C

BML-PW0365-0025   SENP5 (human), pAb   品牌 Biomol

SENP5 (human), pAb

货号: BML-PW0365-0025      产品名称: SENP5 (human), pAb   品牌: Biomol 规格: 25 ul 3周到货 生化实验

Product Specification
Alternative Name:Sentrin-specific protease 5
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 60-77 of human SENP5.
UniProt ID:Q96HI0
Application:Western Blot (1:1’000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human SENP5.
Purity Detail:Purified by salt precipitation.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Store diluted antibody at +4°C, do not freeze and use within 1 month.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage:-20°C
Long Term Storage:-20°C
Miscellaneous/General:SUMO proteases (SENPs) contribute to three distinct activities in the cell, processing SUMO precursors to generate mature SUMO, depolymerization of SUMO chains, and deconjugation of SUMO modified proteins. Cleavage occurs after the conserved SUMO Gly-Gly motif either at the scissile peptide bond during processing or at the scissile isopeptide bond during depolymerization and deconjugation There are 6 SUMO specific human SENP enzymes (1-3, 5-7), each of which share between 20-60% sequence identity to each other within their respective catalytic domains. The deSUMOylating enzyme SENP5 is localised primarily in the nucleoli (though a significant amount is present in the cytosol) and has been shown to be essential for cell cycle progression, relocalising to the mitochondrial surface during mitosis, at the G2/M transition prior to nuclear envelope breakdown, resulting in mitochondrial fragmentation. SENP5 shows a preference for deSUMOylation of SUMO-2 and SUMO-3 from substrates relative to SUMO-1, though this isoform specificity may be determined in part by its enhanced cleavage of conjugates linked via specific target protein lysine residue in comparison to others.

-20°C

BML-PW0365-0100   SENP5 (human), pAb   品牌 Biomol

SENP5 (human), pAb

货号: BML-PW0365-0100      产品名称: SENP5 (human), pAb   品牌: Biomol 规格: 100 ul 3周到货 生化实验

Product Specification
Alternative Name:Sentrin-specific protease 5
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 60-77 of human SENP5.
UniProt ID:Q96HI0
Application:Western Blot (1:1’000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human SENP5.
Purity Detail:Purified by salt precipitation.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Store diluted antibody at +4°C, do not freeze and use within 1 month.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage:-20°C
Long Term Storage:-20°C
Miscellaneous/General:SUMO proteases (SENPs) contribute to three distinct activities in the cell, processing SUMO precursors to generate mature SUMO, depolymerization of SUMO chains, and deconjugation of SUMO modified proteins. Cleavage occurs after the conserved SUMO Gly-Gly motif either at the scissile peptide bond during processing or at the scissile isopeptide bond during depolymerization and deconjugation There are 6 SUMO specific human SENP enzymes (1-3, 5-7), each of which share between 20-60% sequence identity to each other within their respective catalytic domains. The deSUMOylating enzyme SENP5 is localised primarily in the nucleoli (though a significant amount is present in the cytosol) and has been shown to be essential for cell cycle progression, relocalising to the mitochondrial surface during mitosis, at the G2/M transition prior to nuclear envelope breakdown, resulting in mitochondrial fragmentation. SENP5 shows a preference for deSUMOylation of SUMO-2 and SUMO-3 from substrates relative to SUMO-1, though this isoform specificity may be determined in part by its enhanced cleavage of conjugates linked via specific target protein lysine residue in comparison to others.

-20°C

BML-PW0370-0025   SENP6 (human), pAb   品牌 Biomol

SENP6 (human), pAb

货号: BML-PW0370-0025      产品名称: SENP6 (human), pAb   品牌: Biomol 规格: 25 ul 3周到货 生化实验

Product Specification
Alternative Name:Sentrin-specific protease 6, SUSP1, SUMO-1-specific protease 1
Host:Sheep
Immunogen:Recombinant human SENP6 (aa 635-1112) corresponding to the catalytic domain.
UniProt ID:Q9GZR1
Application:Western Blot (1:10’000)
Note: Ensure that blocking is performed with 5% dried Milk/PBS/0.05% Tween, not BSA/PBST.
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human SENP6.
Purity Detail:Purified by salt precipitation and immunodepleted for species that cross-react with other SENP family members.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C. Store diluted antibody at +4°C, do not freeze and use within 1 month.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage:+4°C
Long Term Storage:-20°C
Miscellaneous/General:SUMO proteases (SENPs) contribute to two distinct activities in the cell, processing SUMO precursors to generate mature SUMO and deconjugation of SUMO modified proteins. Cleavage occurs after the conserved SUMO Gly-Gly motif either at the scissile peptide bond during processing or at the scissile isopeptide bond during deconjugation. There are 6 SUMO specific human SENP enzymes (1-3, 5-7), each of which share between 20-60% sequence identity to each other within their respective catalytic domains. In contrast the long N-terminal domains show no sequence similarity and are thought to direct subcellular localization. SENP enzymes have been shown to participate in non-redundant cellular functions such as HIF1α stabilization during hypoxia, mitosis and cytokinesis.

-20°C