MitochondrialCplexIV(Human)ActivityAssay

货号： AAMT004-1KIT      产品名称： MitochondrialCplexIV(Human)ActivityAssay   品牌： Merck 规格： *** 3周到货 生化实验

Mitochondrial Complex IV (Human) Activity Assay Kit
Product Description
The Mitochondrial Complex IV (Human) Activity Assay Kit is used to determine the activity of the cytochrome c oxidase enzyme (EC 1.9.3.1) in a human sample. Complex IV is immunocaptured within the wells and activity is determined colorimetrical by following the oxidation of reduced cytochrome c as an absorbance decrease at 550 nm. The overall reaction is as follows:
4 cytochrome c- + 4 H+ + 4 H+(in) + O2 → 4 cytochrome c  + 2 H2O + 4 H+(out)
reduced                    oxidized
↑Abs 550 nm               ↓Abs 550 n
Panel Components
10 ml  Buffer, (72571-10ML)
1 ml Detergent, (72572-1ML)
1 ml Reduced Cytochrome C, (72573-1ML)
1 ea Microplate, (72574-1EA)
*This kit contains sufficient matirials for 96 measurements (one 96-well microplate).
Storage: Store the Buffer, Detergent, and Microplate at 4°C. Store the Reduced Cytochrome C at –20°C. For multiple experiments, Reduced Cytochrome C may be aliquoted and stored at –20°C or preferably at –80°C.
This kit contains the necessary buffer, detergent for sample preparation, and substrate (reduced cytochrome c) for the reaction. The kit also contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.
商店和运输信息
Storage  Multiple storage requirements
Ship  Multiple Storage Conditions