pBLAST49-hAT3
货号： pbla-hat3 产品名称： pBLAST49-hAT3 品牌： Invivogen 规格： 20 μg 三周到货 生化试验
AntiThrombin III
Coding sequence in expression plasmid
Alias: AT3
AntiThrombin III
This gene is available in pUNO1 expression plasmid selectable with Blasticidin.
Human AntiThrombin III (pUNO1-hAT3)
ORF Size: 1395bp
Subclone: BspHI – NheI
Mouse AntiThrombin III (pUNO1-mAT3)
ORF Size: 1398bp
Subclone: BspHI – NheI
2086-1 HAT Activator, CTPB 品牌 Biovision
HAT Activator, CTPB
货号： 2086-1 产品名称： HAT Activator, CTPB 品牌： Biovision 规格： 1 mg 3周到货 生化实验
HAT Activator, CTPB
A potent HAT activator
Product Overview
Product Name: HAT Activator, CTPB
Alternate Name/Synonyms: N-(4-Chloro-3-trifluoromethyl-phenyl)-2-ethoxy-6-pentadecyl-benzamide
Description: A potent activator of p300 HAT (histone acetyltransferase), but not of PCAF (p300/CBP-associated factor) HAT activities.
Peptide Sequence: N/A
Appearance: White to off-white solid
Formulation: N/A
CAS Number: 586976-24-1
Molecular Formula: C₃₁H₄₃ClF₃NO₂
Molecular Weight: 554.13
Purity: ≥97% by ¹H NMR
Solubility:DMSO
Storage Temp.: -20°C
Shipping Conditions: gel pack
Handling: Protect from air and light
SMILES: CCCCCCCCCCCCCCCC1=C(C(=CC=C1)OCC)C(=O)NC2=CC(=C(C=C2)Cl)C(F)(F)F
InChi: InChI=1S/C31H43ClF3NO2/c1-3-5-6-7-8-9-10-11-12-13-14-15-16-18-24-19-17-20-28(38-4-2)29(24)30(37)36-25-21-22-27(32)26(23-25)31(33,34)35/h17,19-23H,3-16,18H2,1-2H3,(H,36,37)
InChi Key: OYBMVMAXKOGYDC-UHFFFAOYSA-N
PubChem CID: 10311918
MDL Number: N/A
USAGE: For Research Use Only! Not For Use in Humans.
-20°C
2086-5 HAT Activator, CTPB 品牌 Biovision
HAT Activator, CTPB
货号： 2086-5 产品名称： HAT Activator, CTPB 品牌： Biovision 规格： 5 mg 3周到货 生化实验
HAT Activator, CTPB
A potent HAT activator
Product Overview
Product Name: HAT Activator, CTPB
Alternate Name/Synonyms: N-(4-Chloro-3-trifluoromethyl-phenyl)-2-ethoxy-6-pentadecyl-benzamide
Description: A potent activator of p300 HAT (histone acetyltransferase), but not of PCAF (p300/CBP-associated factor) HAT activities.
Peptide Sequence: N/A
Appearance: White to off-white solid
Formulation: N/A
CAS Number: 586976-24-1
Molecular Formula: C₃₁H₄₃ClF₃NO₂
Molecular Weight: 554.13
Purity: ≥97% by ¹H NMR
Solubility:DMSO
Storage Temp.: -20°C
Shipping Conditions: gel pack
Handling: Protect from air and light
SMILES: CCCCCCCCCCCCCCCC1=C(C(=CC=C1)OCC)C(=O)NC2=CC(=C(C=C2)Cl)C(F)(F)F
InChi: InChI=1S/C31H43ClF3NO2/c1-3-5-6-7-8-9-10-11-12-13-14-15-16-18-24-19-17-20-28(38-4-2)29(24)30(37)36-25-21-22-27(32)26(23-25)31(33,34)35/h17,19-23H,3-16,18H2,1-2H3,(H,36,37)
InChi Key: OYBMVMAXKOGYDC-UHFFFAOYSA-N
PubChem CID: 10311918
MDL Number: N/A
USAGE: For Research Use Only! Not For Use in Humans.
-20°C
H0262 HAT Media Supplement|HAT 培养基添加剂|现货 品牌 Sigma
HAT Media Supplement|HAT 培养基添加剂|现货
货号： H0262 产品名称： HAT Media Supplement|HAT 培养基添加剂|现货 品牌： Sigma 规格： 1VL原装 现货
Sigma H0262 HAT Media Supplement (50×) Hybri-Max™
lyophilized powder, γ-irradiated, hybridoma tested
Description
Reconstitution Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.
Legal Information Hybri-Max is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Properties
sterility γ-irradiated
form lyophilized powder
total impurities Endotoxin, tested
suitability hybridoma tested
shipped in dry ice
storage temp. −20°C
Safety
Personal Protective Equipment Eyeshields, Gloves, type P2 (EN 143) respirator cartridges
Hazard Codes T
Risk Statements 61-20/21/22
Safety Statements 53-45
WGK Germany 3
-20度
631205 HAT Protein Exp.& Purif.Sys. 品牌 clontech
HAT Protein Exp.& Purif.Sys.
货号： 631205 产品名称： HAT Protein Exp.& Purif.Sys. 品牌： clontech 规格： 1 set
14-418 P300, HAT DOMAIN 50 UG F 品牌 Millipore
P300, HAT DOMAIN 50 UG F
货号： 14-418 产品名称： P300, HAT DOMAIN 50 UG F 品牌： Millipore 规格： EA 三周到货 生化实验
p300, HAT Domain
Description:
p300, HAT Domain
Trade Name:
Upstate (Millipore)
Qty/Pk:
50 µg
Product Overview:
Recombinant GST-fusion protein corresponding to amino acids 1066-1707 of human p300
Applications:
Recombinant GST-fusion protein corresponding to amino acids 1066-1707 of human p300.
Key Applications:
Histone Acetyltransferase Assay (HAT)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CBP (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CBP can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
View All »
UniProt Summary:
FUNCTION: SwissProt: Q09472 # Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat’s transactivating activity and may help inducing chromatin remodeling of proviral genes.
SIZE: 2414 amino acids; 264144 Da
SUBUNIT: Interacts with phosphorylated CREB1 (By similarity). Interacts with DTX1, EID1, ELF3, FEN1, LEF1, NCOA1, NCOA6, NR3C1, PCAF, PELP1, SPIB, SRY, TCF7L2, TP53, SRCAP and TRERF1. The TAZ- type 1 domain interacts with HIF1A. Probably part of a complex with HIF1A and CREBBP. Part of a complex containing CARM1 and NCOA2/GRIP1. Interacts with ING4 and this interaction may be indirect. Interacts with the C-terminal region of CITED4. Interacts with HTLV-1 Tax and p30II. Interacts with and acetylates HIV-1 Tat.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated. & Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1. & Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1.
DISEASE: SwissProt: Q09472 # Defects in EP300 may play a role in epithelial cancer. & Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with MYST3. & Defects in EP300 are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
SIMILARITY: SwissProt: Q09472 ## Contains 1 bromo domain. & Contains 1 KIX domain. & Contains 2 TAZ-type zinc fingers. & Contains 1 ZZ-type zinc finger.
View All »
Species:
Human
Molecular Weight:
100 kDa
Brand Family:
Upstate
Source:
Expressed in E. coli.
Storage Conditions:
-20°C for up to 6 months fr -20℃
17-289 HAT ASSAY KIT 品牌 Millipore
HAT ASSAY KIT
货号： 17-289 产品名称： HAT ASSAY KIT 品牌： Millipore 规格： EA 三周到货 生化实验
HAT Assay Kit
Description:
HAT Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Applications:
Non-Radioactive HAT Indirect ELISA Assay for the detection of acetyl residues on an acetylated Histone H3 or Histone H4 substrate peptide using an active histone acetyltransferase enzyme.
Key Applications:
ELISA
Application Notes:
Nuclear Extract (source of HAT enzyme activity): Prepare nuclear extracts using a modified protocol of Dignam, et al., Nucleic Acids Res. 22: 1475, 1983 (recommended, but other methods may be used). See citations for reference link. Use 1- 20μl per assay point in the HAT Reaction Cocktail. Optimal nuclear extract concentration will need to be determined empirically.
View All »
Species Reactivity:
Mouse
Human
Rat
Pig
Horse
Rabbit
Guinea Pig
Hamster
Non-human Primate
Canine
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. This gene encodes a histone acetylase that contains A, B, and D motifs, present in many N-acetyltransferases, including those that acetylate substrates other than histones. Alternatively spliced transcript variants that encode different isoforms have been identified for this gene.
View All »
UniProt Summary:
FUNCTION: SwissProt: O14929 # May play a role in telomeric silencing. Acetylates soluble but not nucleosomal H4 at ‘Lys-5’ and ‘Lys-12’ and acetylates histone H2A at ‘Lys-5’. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.
SIZE: 419 amino acids; 49513 Da
SUBUNIT: Heteromer of HAT1 and p46/HAT2 subunits.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear in S-phase cells and cytoplasmic.
SIMILARITY: SwissProt: O14929 ## Belongs to the HAT1 family.
View All »
Brand Family:
Upstate
Gene Symbol:
HAT1
Product Name:
HAT Assay Kit
Quality Assurance:
Routinely evaluated in a HAT Assay. Biotinylated Histone H4 or Histone H3 peptides were acetylated with recombinant PCAF (14-309) in the presence of Acetyl-CoA and HAT Assay buffer.
Components:
Streptavidin Coated Microwell Plate (Cat.# 20-183)
Histone H3 Peptide, biotin conjugate (Cat.# 12-403)
Histone H4 Peptide, biotin conjugate (Cat.# 12-405)
HAT Assay Buffer, 5X (Cat.# 20-148)
07-129
TBS, 20X (Cat.# 20-190)
10% BSA in TBS (Cat.# 20-191)
Acetyl-Histone H3 (K9, K14) Peptide biotinylated; Histone H4 (Tetra Ac) Peptide biotinylated; Acetyl-CoA; Goat Anti-Rabbit IgG, HRP-conjugate; TMB (tetramethylbenzidine) Substrate Reagent A and TMB (tetramethylbenzidine) Substrate Reagent B
View All »
UniProt Number:
O14929
Host:
Rabbit
Detection Methods:
Fluorescent
Packaging:
Kit capacity: 96 assays
Entrez Gene Number:
NM_001033085.1
NM_003642.2
RT
17-289FR Hat Assay Kit (-20C) 品牌 Millipore
Hat Assay Kit (-20C)
货号： 17-289FR 产品名称： Hat Assay Kit (-20C) 品牌： Millipore 规格： EA 三周到货 生化实验
HAT Assay Kit
Description:
HAT Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Applications:
Non-Radioactive HAT Indirect ELISA Assay for the detection of acetyl residues on an acetylated Histone H3 or Histone H4 substrate peptide using an active histone acetyltransferase enzyme.
Key Applications:
ELISA
Application Notes:
Nuclear Extract (source of HAT enzyme activity): Prepare nuclear extracts using a modified protocol of Dignam, et al., Nucleic Acids Res. 22: 1475, 1983 (recommended, but other methods may be used). See citations for reference link. Use 1- 20μl per assay point in the HAT Reaction Cocktail. Optimal nuclear extract concentration will need to be determined empirically.
View All »
Species Reactivity:
Mouse
Human
Rat
Pig
Horse
Rabbit
Guinea Pig
Hamster
Non-human Primate
Canine
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. This gene encodes a histone acetylase that contains A, B, and D motifs, present in many N-acetyltransferases, including those that acetylate substrates other than histones. Alternatively spliced transcript variants that encode different isoforms have been identified for this gene.
View All »
UniProt Summary:
FUNCTION: SwissProt: O14929 # May play a role in telomeric silencing. Acetylates soluble but not nucleosomal H4 at ‘Lys-5’ and ‘Lys-12’ and acetylates histone H2A at ‘Lys-5’. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.
SIZE: 419 amino acids; 49513 Da
SUBUNIT: Heteromer of HAT1 and p46/HAT2 subunits.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear in S-phase cells and cytoplasmic.
SIMILARITY: SwissProt: O14929 ## Belongs to the HAT1 family.
View All »
Brand Family:
Upstate
Gene Symbol:
HAT1
Product Name:
HAT Assay Kit
Quality Assurance:
Routinely evaluated in a HAT Assay. Biotinylated Histone H4 or Histone H3 peptides were acetylated with recombinant PCAF (14-309) in the presence of Acetyl-CoA and HAT Assay buffer.
Components:
Streptavidin Coated Microwell Plate (Cat.# 20-183)
Histone H3 Peptide, biotin conjugate (Cat.# 12-403)
Histone H4 Peptide, biotin conjugate (Cat.# 12-405)
HAT Assay Buffer, 5X (Cat.# 20-148)
07-129
TBS, 20X (Cat.# 20-190)
10% BSA in TBS (Cat.# 20-191)
Acetyl-Histone H3 (K9, K14) Peptide biotinylated; Histone H4 (Tetra Ac) Peptide biotinylated; Acetyl-CoA; Goat Anti-Rabbit IgG, HRP-conjugate; TMB (tetramethylbenzidine) Substrate Reagent A and TMB (tetramethylbenzidine) Substrate Reagent B
View All »
UniProt Number:
O14929
Host:
Rabbit
Detection Methods:
Fluorescent
Packaging:
Kit capacity: 96 assays
Entrez Gene Number:
NM_001033085.1
NM_003642.2
RT
17-289RF Hat Assay Kit (2-8C) 品牌 Millipore
Hat Assay Kit (2-8C)
货号： 17-289RF 产品名称： Hat Assay Kit (2-8C) 品牌： Millipore 规格： EA 三周到货 生化实验
HAT Assay Kit
Description:
HAT Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Applications:
Non-Radioactive HAT Indirect ELISA Assay for the detection of acetyl residues on an acetylated Histone H3 or Histone H4 substrate peptide using an active histone acetyltransferase enzyme.
Key Applications:
ELISA
Application Notes:
Nuclear Extract (source of HAT enzyme activity): Prepare nuclear extracts using a modified protocol of Dignam, et al., Nucleic Acids Res. 22: 1475, 1983 (recommended, but other methods may be used). See citations for reference link. Use 1- 20μl per assay point in the HAT Reaction Cocktail. Optimal nuclear extract concentration will need to be determined empirically.
View All »
Species Reactivity:
Mouse
Human
Rat
Pig
Horse
Rabbit
Guinea Pig
Hamster
Non-human Primate
Canine
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. This gene encodes a histone acetylase that contains A, B, and D motifs, present in many N-acetyltransferases, including those that acetylate substrates other than histones. Alternatively spliced transcript variants that encode different isoforms have been identified for this gene.
View All »
UniProt Summary:
FUNCTION: SwissProt: O14929 # May play a role in telomeric silencing. Acetylates soluble but not nucleosomal H4 at ‘Lys-5’ and ‘Lys-12’ and acetylates histone H2A at ‘Lys-5’. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.
SIZE: 419 amino acids; 49513 Da
SUBUNIT: Heteromer of HAT1 and p46/HAT2 subunits.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear in S-phase cells and cytoplasmic.
SIMILARITY: SwissProt: O14929 ## Belongs to the HAT1 family.
View All »
Brand Family:
Upstate
Gene Symbol:
HAT1
Product Name:
HAT Assay Kit
Quality Assurance:
Routinely evaluated in a HAT Assay. Biotinylated Histone H4 or Histone H3 peptides were acetylated with recombinant PCAF (14-309) in the presence of Acetyl-CoA and HAT Assay buffer.
Components:
Streptavidin Coated Microwell Plate (Cat.# 20-183)
Histone H3 Peptide, biotin conjugate (Cat.# 12-403)
Histone H4 Peptide, biotin conjugate (Cat.# 12-405)
HAT Assay Buffer, 5X (Cat.# 20-148)
07-129
TBS, 20X (Cat.# 20-190)
10% BSA in TBS (Cat.# 20-191)
Acetyl-Histone H3 (K9, K14) Peptide biotinylated; Histone H4 (Tetra Ac) Peptide biotinylated; Acetyl-CoA; Goat Anti-Rabbit IgG, HRP-conjugate; TMB (tetramethylbenzidine) Substrate Reagent A and TMB (tetramethylbenzidine) Substrate Reagent B
View All »
UniProt Number:
O14929
Host:
Rabbit
Detection Methods:
Fluorescent
Packaging:
Kit capacity: 96 assays
Entrez Gene Number:
NM_001033085.1
NM_003642.2
RT
17-329 HAT ASSAY REAGENT KIT 100ASSY 品牌 Millipore
HAT ASSAY REAGENT KIT 100ASSY
货号： 17-329 产品名称： HAT ASSAY REAGENT KIT 100ASSY 品牌： Millipore 规格： EA 三周到货 生化实验
HAT Assay Reagent Kit
Description:
HAT Assay Reagent Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
Accessory reagent kit for the analysis of histone acetyltransferase activity.
Applications:
Accessory reagent kit for the analysis of histone acetyltransferase activity.
Key Applications:
Histone Acetyltransferase Assay (HAT)
Application Notes:
Nuclear Extract (source of HAT enzyme activity): Prepare nuclear extracts using a modified protocol of Dignam, et al., Nucleic Acids Res. 22: 1475, 1983 (recommended, but other methods may be used). Use 1- 20μl per assay point in the HAT Reaction Cocktail below. Optimal nuclear extract concentration will need to be determined empirically.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. This gene encodes a histone acetylase that contains A, B, and D motifs, present in many N-acetyltransferases, including those that acetylate substrates other than histones. Alternatively spliced transcript variants that encode different isoforms have been identified for this gene.
View All »
UniProt Summary:
FUNCTION: SwissProt: O14929 # May play a role in telomeric silencing. Acetylates soluble but not nucleosomal H4 at ‘Lys-5’ and ‘Lys-12’ and acetylates histone H2A at ‘Lys-5’. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.
SIZE: 419 amino acids; 49513 Da
SUBUNIT: Heteromer of HAT1 and p46/HAT2 subunits.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear in S-phase cells and cytoplasmic.
SIMILARITY: SwissProt: O14929 ## Belongs to the HAT1 family.
View All »
Brand Family:
Upstate
Gene Symbol:
HAT1
Product Name:
HAT Assay Reagent Kit
Quality Assurance:
Routinely evaluated by incubating pCAF, active (14-309) with Core Histones (13-107B). Reactions were terminated at indicated times and acetyl incorporation was measured.
Components:
HAT Assay Buffer, 5X
Core Histones
P81 Phosphocellulose squares
Acetyl-CoA
UniProt Number:
O14929
Packaging:
Kit capacity: 100 assays
Entrez Gene Number:
NM_001033085.1
NM_003642.2
RT
20-148 HAT ASSAY BUFFER, 5X 2M 品牌 Millipore
HAT ASSAY BUFFER, 5X 2M
货号： 20-148 产品名称： HAT ASSAY BUFFER, 5X 2M 品牌： Millipore 规格： EA 三周到货 生化实验
HAT Assay Buffer, 5X
Description:
HAT Assay Buffer, 5X
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Applications:
For use in the HAT Assay.
Key Applications:
Histone Acetyltransferase Assay (HAT)
Application Notes:
For use in the HAT Assay.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Quality Assurance:
Routinely evaluated in a HAT Assay
Brand Family:
Upstate
Presentation:
250mM Tris-base, pH 8.0, 50% glycerol, 0.5mM EDTA and 5mM dithiothreitol
Storage Conditions:
2 years at -20°C
Product Name:
HAT Assay Buffer, 5X
-20℃
ALX-850-326-KI01 HAT Activity Assay Kit 品牌 Alexis
HAT Activity Assay Kit
货号： ALX-850-326-KI01 产品名称： HAT Activity Assay Kit 品牌： Alexis 规格： 1 Kit 3周到货 生化实验
HAT activity assay kit
Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active recombinant HAT as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
Figure 1: Analysis of HAT Activity in HeLa Nuclear Extract. HeLa nuclear extract in various amounts was incubated with HAT substrate. Activity was analyzed in a microplate reader at 440nm according to the kit instructions.Please mouse over
Product Specification
Long Term Storage: -20°C
-20°C