标签归档：glucokinase

7776-10   Glucokinase, human liver, recombinant   品牌 Biovision

发表于由

Glucokinase, human liver, recombinant

货号： 7776-10      产品名称： Glucokinase, human liver, recombinant   品牌： Biovision 规格： 10 μg 3周到货 生化实验

Glucokinase, human liver, recombinant
Plays a key role in the communication of cells with their microenvironment
Product Overview
Alternate Name/Synonyms: Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D
Gene Symbol: GCK
Accession #: P35557
Gene ID: 2645
Source: E. coli.
Appearance: Liquid
Physical Form Description: 50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
Molecular Weight: 53.4 kDa
Purity by SDS-PAGE: ≥77%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 662 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30°C for 30 min.
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry Ice
Background Information: Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human liver Glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-80°C

7777-10   Glucokinase, human pancreatic, recombinant   品牌 Biovision

发表于由

Glucokinase, human pancreatic, recombinant

货号： 7777-10      产品名称： Glucokinase, human pancreatic, recombinant   品牌： Biovision 规格： 10 μg 3周到货 生化实验

Glucokinase, human pancreatic, recombinant
Plays an important role in the regulation of carbohydrate metabolism
Product Overview
Alternate Name/Synonyms: Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D
Gene Symbol: GCK
Accession #: P35557
Gene ID: 2645
Source: E. coli.
Appearance: Liquid
Physical Form Description: 50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
Molecular Weight: 53.1 kDa
Purity by SDS-PAGE: ≥80%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 303 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry Ice
Background Information: Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-80°C

BML-SE408-0020   Glucokinase (human) (recombinant)   品牌 Biomol

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Glucokinase (human) (recombinant)

货号： BML-SE408-0020      产品名称： Glucokinase (human) (recombinant)   品牌： Biomol 规格： 20 ug 3周到货 生化实验

Glucokinase (human) (recombinant)
Product Specification
Alternative Name:Hexakinase D
MW:51 kDa
Source:Produced in E. coli. Full length active enzyme.
UniProt ID:P35557
Long Term Storage:-80°C
Miscellaneous/General:Glucokinase phosphorylates carbon 6 on glucose in the first step in glycolysis. This enzyme is only expressed in the liver and the β cells of the pancreas, where it plays a key role in regulating metabolism.

-80°C

3153-100   GCK (Glucokinase) Antibody   品牌 Biovision

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GCK (Glucokinase) Antibody

货号： 3153-100      产品名称： GCK (Glucokinase) Antibody   品牌： Biovision 规格： 100 μg 3周到货 生化实验

GCK (Glucokinase) Antibody
Product Overview
Antibody Target: GCK (Glucokinase)
Alternative Name: Hexokinase D, Hexokinase type IV, HK IV, HK4, Glucokinase
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide selected from the N-terminal region of human GCK
Accession#: P35557
Gene ID: 2645
Appearance: Colorless liquid
Concentration: 0.25 mg/ml
Formulation: 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification: Affinity purified
Species Reactivity: human, mouse
Application: ELISA, Western blot, Immunohistochemistry, Flow cytometry, Immunofluorescence
Application & Usage: The antibody can be used for ELISA (0.25 µg/ml), Western Blotting (0.5 – 2.5 µg/ml), Immunohistochemistry (2.5 – 5.0 µg/ml), Immunoflurescence (1:10 – 1:50), and Flow cytometry.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), also called maturity onset diabetes of the young, type 2 (MODY2); mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.

-20°C

3154-100   GCK (Glucokinase) Antibody   品牌 Biovision

发表于由

GCK (Glucokinase) Antibody

货号： 3154-100      产品名称： GCK (Glucokinase) Antibody   品牌： Biovision 规格： 100 μg 3周到货 生化实验

GCK (Glucokinase) Antibody
Product Overview
Antibody Target: GCK (Glucokinase)
Alternative Name: Hexokinase D, Hexokinase type IV, HK IV, HK4, Glucokinase
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Accession#: P35557
Gene ID: 2645
Appearance: Colorless liquid
Concentration: 0.25 mg/ml
Immunogen: KLH conjugated synthetic peptide between 411 and 441 amino acids from the C-terminal region of human GCK.
Formulation: 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification: Affinity purified
Species Reactivity: human
Application: ELISA, Western blot, Immunohistochemistry
Application & Usage: The antibody can be used for ELISA (1:1000), Western blotting (1:100-500), Immunohistochemistry (1:50-100).
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), also called maturity onset diabetes of the young, type 2 (MODY2); mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.

-20°C