Glucokinase, human pancreatic, recombinant

货号： 7777-10      产品名称： Glucokinase, human pancreatic, recombinant   品牌： Biovision 规格： 10 μg 3周到货 生化实验

Glucokinase, human pancreatic, recombinant
Plays an important role in the regulation of carbohydrate metabolism
Product Overview
Alternate Name/Synonyms: Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D
Gene Symbol: GCK
Accession #: P35557
Gene ID: 2645
Source: E. coli.
Appearance: Liquid
Physical Form Description: 50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
Molecular Weight: 53.1 kDa
Purity by SDS-PAGE: ≥80%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 303 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry Ice
Background Information: Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-80°C