FRACTIN, RBX-100UL

货号： AB3150      产品名称： FRACTIN, RBX-100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Fractin Antibody, CT
Close
REFERENCES
Natural and lesion-induced apoptosis in the rat striatum during development.
Mellios, K, et al. (2009) Brain Res., 1252: 30-44 (2009)
Apoptotic and necrotic death mechanisms are concomitantly activated in the same cell after cerebral ischemia.
Unal-Cevik, Isin, et al. (2004) Stroke, 35: 2189-94 (2004)
Distinct nuclear and cytoplasmic localization of caspase cleavage products in two models of induced apoptotic death in dopamine neurons of the substantia nigra.
Oo, Tinmarla F, et al. (2002) Exp. Neurol., 175: 1-9 (2002)
Nitric oxide is involved in ischemia-induced apoptosis in brain: a study in neuronal nitric oxide synthase null mice.
Elibol, B, et al. (2001) Neuroscience, 105: 79-86 (2001)
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, ICC, IH(P) Rabbit Serum Polyclonal Antibody
Description:
Anti-Fractin Antibody, CT
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
By immunohistochemistry and Western blot, this antibody only recognizes the 32 kDa Fractin fragment but not full-length beta actin.
Epitope:
C-terminus
Immunogen:
K-YELPD peptide representing the last five amino acids of the C-terminus of the 32 kDa Fractin fragment of beta-Actin. This sequence is absolutely conserved in Fractin fragments from human, mouse, rat, feline, porcine, ovine, bovine, canine, rabbit and guinea pig origin.
Background Information:
During apoptosis, beta actin is cleaved by activated caspase 3 between Asp244 and Gly245. This cleavage results in the release of an approximately 130 amino acid (15 kDa) fragment from the C-terminus of beta actin and a 32 kDa (244 amino acid) N-terminal fragment of actin, known as Fractin. The C-terminus of the Fractin fragment represents an apoptosis-specific neoepitope that is not antigenic in native full-length beta actin and therefore presents a novel and specific marker for distinguishing apoptotic from necrotic cells.
View All »
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Immunohistochemistry/Immunocytochemistry: 1:400-1:1000. 1 hr at 37°C or 4°C overnight. Note: If studying paraffin-embedded tissue, the use of antigen retrieval methods is recommended.
Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4
Optimal working dilutions must be determined by end user.
View All »
Control:
POSITIVE CONTROL: Staurosporin treated neuronal cultures.
Presentation:
Liquid whole rabbit serum containing no preservatives.
Storage Conditions:
Aliquot and store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.
UniProt Number:
P60709
Entrez Gene Number:
NM_001101.2
Gene Symbol:
ACTB
PS1TP5BP1
beta-actin
Beta-actin
Alternate Names:
32 kDa Fragment of beta-Actin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene encodes one of six different actin prot -20℃