Anti-Cohesin subunit SA-2

货号： ABE394      产品名称： Anti-Cohesin subunit SA-2   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Cohesin subunit SA-2
Species Reactivity Key Applications Host Format Antibody Type
H  IP, WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Cohesin subunit SA-2 Antibody
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Molecular Weight:
~140 kDa observed. An uncharacterized band may appear at ~35 kDa in some lysates. Uncharacterized bands may appear at ~40 kDa and ~30 kDa in Immunoprecipitation.
Immunogen:
KLH-conjugated linear peptide corresponding to human Cohesin Subunit SA-2.
Background Information:
The cohesin subunit SA-2 is a part of the cohesin complex. This complex is comprised of a heterodimer between SMC1 and SMC3 proteins, as well as RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a large ring that can trap sister chromatids after DNA replication. During prophase, the cohesion complexes dissociate from the chromatin due to the phosphorylation by PLK1 (Polo-like kinase) with the exception at the centromeres where the cohesion complexes remain. Then during anaphase, the RAD21 subunit is cleaved which causes the dissociation of the complex from chromosomes, allowing chromosome separation.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Rhesus Macaque, Equine, and Bovine based on 100% sequence homology. Other homologies: Mouse and Rat (93%-14/15aa).
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Cohesin Subunit SA-2 in A431 and HeLa cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cohesin Subunit SA-2 from HeLa cell lysate.
Control:
HeLa cell nuclear extract
Quality Assurance:
Evaluated by Western Blot in HeLa cell nuclear extract.
Western Blot Analysis: 0.2 µg/mL of this antibody detected Cohesin Subunit SA-2 in 10 µg of HeLa cell nuclear extract.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q8N3U4
Entrez Gene Number:
NP_006594
Gene Symbol:
STAG2
SA2
Alternate Names:
Cohesin subunit SA-2
SCC3 homolog 2
Stromal antigen 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Immunocytochemistry
Product Name:
Anti-Cohesin subunit SA-2
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect Cohesin subunit SA-2 using this Anti-Cohesin subunit SA-2 Antibody validated for use in 0.

2-8℃