标签归档：catalytic

PF117-10UG   MT5-MMP CATALYTIC DOMAIN, HIS-TAG, REC.   品牌 Merck

发表于由

MT5-MMP CATALYTIC DOMAIN, HIS-TAG, REC.

货号： PF117-10UG      产品名称： MT5-MMP CATALYTIC DOMAIN, HIS-TAG, REC.   品牌： Merck 规格： *** 3周到货 生化实验

MT5-MMP, Catalytic Domain, His•Tag®, Human, Recombinant, E. coli
MMP-24, Catalytic Domain
Recombinant, human pro-MT5-MMP fused to a His•Tag® sequence, expressed in E. coli and activated to yield the active catalytic domain. Two isoforms are produced, one with an N-terminal Tyr156, the other with an N-terminal Leu158. Useful for the study of the activation of progelatinase A and the degradation of extracellular matrix proteins. MT5-MMP is a member of the Zn2+- and Ca2+-dependent endopeptidases that functions in the degradation of extracellular matrix components such as gelatin, chondroitin sulfate, and dermatan sulfate. It has a tendency to be shed from cell surface membranes and appears to undergo rapid autocatalytic destruction into smaller fragments.
产品信息
Form  Liquid
Formulation  In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl2, pH 7.5.
Preservative  None
Molar mass  22,000
Avoid freeze/thaw  是的
Specific activity  ≥100 mU/mg protein
Unit definition  One unit is defined as the amount of enzyme necessary to hydrolyze 1.0 μmol of the substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg (Cat. No. 03-32-5032) per minute at 37°C, pH 7.5.
Purity  ≥95% by SDS-PAGE
商店和运输信息
Storage  ≤ -70°C
Ship  Dry Ice Only
Standard Handling

≤ -70°C

BML-SA415-0100   ADAM17 (catalytic domain), pAb   品牌 Biomol

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ADAM17 (catalytic domain), pAb

货号： BML-SA415-0100      产品名称： ADAM17 (catalytic domain), pAb   品牌： Biomol 规格： 100 ul 3周到货 生化实验

ADAM17 (catalytic domain), pAb
Product Specification
Alternative Name:TACE, A disintegrin and metalloproteinase 17
Host:Rabbit
Immunogen:Recombinant human ADAM17 (TACE) catalytic domain.
UniProt ID:P78536
Application:Western Blot
Optimal conditions must be determined individually for each application.
Specificity:Recognizes all latent and active forms of human ADAM17. Does not cross-react with ADAM10.
Formulation:Liquid. Neat serum.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-80°C

-80°C

BML-SA470-0100   ADAM12 (catalytic domain) (human), pAb   品牌 Biomol

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ADAM12 (catalytic domain) (human), pAb

货号： BML-SA470-0100      产品名称： ADAM12 (catalytic domain) (human), pAb   品牌： Biomol 规格： 100 ul 3周到货 生化实验

ADAM12 (catalytic domain) (human), pAb
Product Specification
Alternative Name:A disintegrin and metalloproteinase 12, Meltrin α
Host:Rabbit
Immunogen:Recombinant human ADAM12 catalytic domain.
UniProt ID:O43184
Application:Western Blot
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human ADAM12 (catalytic domain).
Formulation:Liquid. Neat serum.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-80°C

-80°C

BML-SE255-0010   MMP-8 Catalytic Domain (human) (recombinant)   品牌 Biomol

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MMP-8 Catalytic Domain (human) (recombinant)

货号： BML-SE255-0010      产品名称： MMP-8 Catalytic Domain (human) (recombinant)   品牌： Biomol 规格： 10 ug 3周到货 生化实验

MMP-8 (catalytic domain) (human), (recombinant)
Product Specification
Alternative Name:Matrix metalloproteinase 8, Neutrophil collagenase, Collagenase-2
MW:20.3 kDa
Source:Produced in E. coli. Active Matrix Metalloproteinase-8 (MMP-8, neutrophil collagenase, collagenase-2) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-8 (Phe99-Gln269, NM_002424) with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-8 which lacks the C-terminal hemopexin domain1. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected.
UniProt ID:P22894
Formulation:Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20µM ZnCl2, 0.5% Brij-35, and 30% glycerol.
Purity:≥90% (SDS-PAGE)
Purity Detail:Purified by multi-step chromatography.
Activity:Preincubation of MMP-8 catalytic domain at 13.6 nM with the broad-spectrum inhibitor GM6001 (Prod #BML-EI300) at 20nM for 1 hour completely inhibits enzymatic activity.
Specific Activity:≥150 U/µg. One U=100 pmol/min at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100 µM; Prod. No. BML-P125) as substrate.
Application:Study enzyme kinetics, cleave target substrates, and screen for inhibitors.
Long Term Storage:-80°C

-80°C

03-188   p300(catalytic domain)human recombinant   品牌 Millipore

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p300(catalytic domain)human recombinant

货号： 03-188      产品名称： p300(catalytic domain)human recombinant   品牌： Millipore 规格： EA 3周到货 生化实验

p300 (catalytic domain) human recombinant
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PRODUCT FAMILY INFORMATION
Recombinant Histone Proteins, Enzymes, Peptides & Inhibitors
Purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates and inhibitors.
Perform intricate in vitro manipulations of histones, nucleosomes, and chromatin with our portfolio of purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates.
» More…
Description:
p300 (catalytic domain) human recombinant
Trade Name:
Upstate (Millipore)
Qty/Pk:
100 µg
Product Overview:
Human recombinant p300 (catalytic domain) expressed in E. Coli
Background Information:
Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase ß and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor.
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Applications:
Human recombinant p300 (catalytic domain) expressed in E. Coli.
Key Applications:
Enzyme Assay
Application Notes:
Acetyltransferase assays
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Species:
Human
Molecular Weight:
~52 kDa
Brand Family:
Upstate
Source:
Catalytic domain of huma p300
Presentation:
In liquid containing 50 mM Tris/HCl, pH 8.0, 0.1 mM EDTA, 10% glycerol
Storage Conditions:
Stable for 12 months at -80ºC from date of receipt. Avoid multiple freeze/thaw cycles.
Product Name:
p300 (catalytic domain) human recombinant
Physical Form:
Frozen solution
Format:
Purified
Host:
E. coli
Specific Activity:
>250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386. CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis (2-nitrobenzoic acid).
Purity:
Purity >75% by SDS-PAGE

-80°C

03-189   CBP (catalytic domain) human recombinant   品牌 Millipore

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CBP (catalytic domain) human recombinant

货号： 03-189      产品名称： CBP (catalytic domain) human recombinant   品牌： Millipore 规格： EA 3周到货 生化实验

CBP (catalytic domain) human recombinant
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PRODUCT FAMILY INFORMATION
Recombinant Histone Proteins, Enzymes, Peptides & Inhibitors
Purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates and inhibitors.
Perform intricate in vitro manipulations of histones, nucleosomes, and chromatin with our portfolio of purified recombinant histones, native nucleosomes, highly active histone-modifying enzymes, and broad range of modified peptide substrates.
» More…
Description:
CBP (catalytic domain) human recombinant
Trade Name:
Upstate (Millipore)
Qty/Pk:
100 µg
Product Overview:
CBP (catalytic domain) human recombinant expressed in E. Coli
Background Information:
Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase ß and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor.
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Applications:
CBP (catalytic domain) human recombinant protein expressed in E. Coli.
Key Applications:
Enzyme Assay
Application Notes:
Acetyltransferase assays
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Species:
Human
Molecular Weight:
~48 kDa
Brand Family:
Upstate
Source:
Catalytic domain (residues 1319-1710) of human CBP histone acetyltransferase
Presentation:
In liquid containing 50 mM Tris/HCl, pH 8.0, 0.1 mM EDTA, 10% glycerol.
Storage Conditions:
Stable for 12 months at -80°C from date of receipt. Avoid multiple freeze/thaw cycles.
Product Name:
CBP (catalytic domain) human recombinant
Physical Form:
Frozen solution
Format:
Purified
UniProt Number:
Q92793
Specific Activity:
[Varies with lot. For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.] XXX 250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386. CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis (2-nitrobenzoic acid).
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Purity:
Purity >65% by SDS-PAGE

-80°C

BML-UW9760-0100   SENP1 (Catalytic Domain) (human) (recombinant) (GST)   品牌 Biomol

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SENP1 (Catalytic Domain) (human) (recombinant) (GST)

货号： BML-UW9760-0100      产品名称： SENP1 (Catalytic Domain) (human) (recombinant) (GST)   品牌： Biomol 规格： 100 ug 3周到货 生化试验

SENP1 (catalytic domain) (human), (recombinant) (GST-tag)
Product Specification
Alternative Name: Sentrin-specific protease 1
Source: Produced in E. coli.
UniProt ID: Q9P0U3
Formulation: Liquid. In 50mM Tris, 150mM NaCl, pH 7.5, containing 0.5mM DTT.
Biological Activity: This fragment has full de-SUMOylating activity.
Long Term Storage: -80°C
Miscellaneous/General: SENP1 fragment has been reported to co-localize with a herpes virus protein ICP0 during early infection. This co-localization may be involved in the ICP0-induced loss of SUMO-1-modified PML in infected cells. Characterization of the localization and regulation of SENP1 has also been reported. SENP1 localization may also be influenced by the expression of proteins that are targets of SUMO-1 modification, such as HDAC4 and PML, which affect the relative levels and localization of SUMO-1 conjugates within the cell.

-80°C

BML-UW9765-0100   SENP2 (Catalytic Domain) (human) (recombinant) (GST)   品牌 Biomol

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SENP2 (Catalytic Domain) (human) (recombinant) (GST)

货号： BML-UW9765-0100      产品名称： SENP2 (Catalytic Domain) (human) (recombinant) (GST)   品牌： Biomol 规格： 100 ug 3周到货 生化试验

SENP2 (catalytic domain) (human), (recombinant) (GST-tag)
Product Specification
Alternative Name: Sentrin-specific protease 2
Sequence: Residues 368-549 of SENP2 (catalytic domain) fused at the N-terminus to a GST-tag.
MW: ~52kDa
Source: Produced in E. coli.
UniProt ID: Q9HC62
Formulation: Liquid. In 50mM TRIS, pH 7.5, containing 150mM sodium chloride, 10% glycerol, and 0.5mM DTT.
Purity: ≥90% (densitometry)
Purity Detail: Purified by multi-step chromatography.
Specificity: Demonstrated recognition of SUMO-1 and SUMO-2 in vitro
Biological Activity: This fragment has full de-SUMOylating activity.
Application: Activity Assay (deSUMOylation)
Long Term Storage: -80°C

-80°C

BML-SE268-0010   ADAM17 (catalytic domain) (human), (recombinant) (His-tag)   品牌 Biomol

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ADAM17 (catalytic domain) (human), (recombinant) (His-tag)

货号： BML-SE268-0010      产品名称： ADAM17 (catalytic domain) (human), (recombinant) (His-tag)   品牌： Biomol 规格： 10 ug 3周到货 生化实验

ADAM17 (catalytic domain) (human), (recombinant) (His-tag)
Product Specification
Alternative Name:TACE, A disintegrin and metalloproteinase 17, Tumor necrosis factor-α-converting enzyme
Sequence: 
Recombinant glycosylated catalytic domain (aa Pro1-Val477) of ADAM17/TACE (A disintegrin and metalloproteinase 17; Tumor necrosis factor-α-converting enzyme), cloned from human cDNA (NM_003183), secreted as mature, active enzyme from insect cells, and purified using a C-terminal His-tag.
MW:~30.5kDa (calculated), ~36kDa doublet (SDS-PAGE)
Source:Produced in insect cells.
UniProt ID:P78536
Formulation:Liquid. In 22.5mM TRIS, pH 7.5, containing 4.5µM ZnCl2, 0.0045% Brij-35 and 10% glycerol.
Purity:≥90% (SDS-PAGE)
Purity Detail:Purified by multi-step chromatography.
Specific Activity:≥1800 U/µg enzyme. One unit will hydrolyze one pmole Mca-PLAQAV-Dpa-RSSSR-NH2 substrate (Prod. No. BML-P132) (10µM) per minute at 37°C, in 25mM TRIS, pH 9.0.
Application:Study enzyme kinetics, cleave target substrates, screen inhibitors.
Long Term Storage:-80°C
Use/Stability:Salts (sodium chloride, calcium chloride, etc.) in the assay are inhibitory.ADAM17/TACE is stable after 6 freeze-thaws at ~0.4µg/µl; freeze-thaw stability of more dilute preparations has not been tested and could lead to loss of activity.

-80°C

BML-UW1015-0100   A20 (catalytic domain) (human), (recombinant) (untagged)   品牌 Biomol

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A20 (catalytic domain) (human), (recombinant) (untagged)

货号： BML-UW1015-0100      产品名称： A20 (catalytic domain) (human), (recombinant) (untagged)   品牌： Biomol 规格： 100 ug 3周到货 生化试验

A20 (catalytic domain) (human), (recombinant) (untagged)
Product Specification
Alternative Name: Tumor necrosis factor alpha-induced protein 3
, TNAP3, TNFAIP3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20
MW: ~43kDa
Source: Produced in E. coli.
UniProt ID: P21580
Formulation: Liquid. In 25mM TRIS-HCl, pH 8.5, containing 200mM sodium chloride, 1mM EDTA and 5mM DTT.
Purity: ≥80% (SDS-PAGE)
Long Term Storage: -80°C
Use/Stability: Stable for at least 1 year after receipt when stored at -80°C.
Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

-80°C

07-1216   Anti-PP2A, Catalytic subunit (rabbit polyclonal)   品牌 Millipore

发表于由

Anti-PP2A, Catalytic subunit (rabbit polyclonal)

货号： 07-1216      产品名称： Anti-PP2A, Catalytic subunit (rabbit polyclonal)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-PP2A Antibody, Catalytic subunit
Recommended Replacement for: 07-1216 is a recommended replacement for AB1621.
Species Reactivity Key Applications Host Format Antibody Type
B, Ch, H, M, R  WB, IH(P) Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-PP2A Antibody, Catalytic subunit
Replaces:
07-1216 is a recommended replacement for AB1621.
Promotional Text:
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100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes protein phosphatase 2A, catalytic subunit.
Molecular Weight:
60 kDa
Epitope:
C-terminus
Immunogen:
Synthetic peptide corresponding to a.a. 298-309 of the human sequence.
Isotype:
IgG
Background Information:
PP2A is a type 2 protein Ser/Thr phosphatase characterized by high activity against the α subunit of Phosphorylase Kinase and low sensitivity to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades. PP2A is a heterotrimer containing a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B’/PR61, and B”/PR72 . PTPA is a member of the B’ family that is required for activation of PP2A by an undefined mechanism.
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Species Reactivity:
Bovine
Chicken
Human
Mouse
Rat
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Species Reactivity Note:
Peptide is highly conserved in human, rat, bovine, mouse and chicken.
Application Notes:
Immunohistochemisty (paraffin): Antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as staining in normal human testis.
Control:
HEK293 cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500-1:1,000 dilution is traditonally used to detected PP2A, Catalytic subunit in 10 μg of HEK293 lysates.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal IgG in buffer containing PBS with no preservatives.
Storage Conditions:
Stable for 1 year at -80°C from date of receipt. Avoid repeated freeze/thaw.
UniProt Number:
Q14738
Entrez Gene Number:
NM_006245
Gene Symbol:
PPP2R5D
MGC8949
MGC2134
OTTHUMP00000039821
B56D
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Alternate Names:
Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform
Delta isoform of regulatory subunit B56, protein phosphatase
Protein phosphatase 2, regulatory subunit B (B56), delta isoform
Protein phosphatase 2, regulatory subunit B’, delta isoform
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It -80°C

AB6005   Anti-MT1-MMP, catalytic domain   品牌 Millipore

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Anti-MT1-MMP, catalytic domain

货号： AB6005      产品名称： Anti-MT1-MMP, catalytic domain   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-MT1-MMP, catalytic domain
Recommended Replacement for: AB8102
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100% Performance Guaranteed
Species Reactivity Key Applications Host Format Antibody Type
H, Chp  WB, ICC, IHC, IP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-MT1-MMP Antibody, catalytic domain
Replaces:
AB8102
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
The antibody recognizes MT1-MMP (MMP-14).
Molecular Weight:
~ 65 kDa
Epitope:
Catalytic domain
Immunogen:
GST-tagged recombinant protein corresponding to the catalytic domain of human MT1-MMP.
Background Information:
Matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes degrade components of extracellular matrix (ECM), including collagens, fibronectin, laminin and other glycoproteins. MMPs play an important role in wound healing, apoptosis, development, angiogenesis, cancer metastases, and tissue remodeling. Most MMPs are secreted as inactive proproteins which are cleaved and activated; however, MT1-MMP (MMP-14) is a member of the membrane-type subfamily. Each member of this subfamily has a transmembrane domain suggesting these proteins are expressed at the cell’s surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of ECM components and thought to be an important tool for cellular remodeling of the ECM. It is also involved in endothelial cell migration, and although not fully characterized, MT1-MMP activity appears to modulate endothelial invasion, and formation of capillary tubes during angiogenesis (Galvez, 2001). It also appears to be a factor in monocyte recruitment during inflammation (Matias-Roman, 2005). MT1-MMP also activates MMP-2 protein, an activity involved in tumor invasion.
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Species Reactivity:
Human
Chimpanzee
Species Reactivity Note:
Demonstrated to react with human. Predicted to react with chimpanzee based on 100% sequence homology.
Equine, sheep, porcine, canine, and bovine (99% sequence homology). Mouse and rat (97% sequence homology). Orangutan (92% sequence homology).
Application Notes:
Western Blot Analysis: 0.5 µg/mL from a previous lot detected MT1-MMP in 10 µg of human normal uterus tissue lysate.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected MT1-MMP in prostate carcinoma tissue.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated MT1-MMP from 500 µg of human normal uterus RIPA lysate.
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Control:
HT-1080 cell lysate
Quality Assurance:
Evaluated by Western Blot in HT-1080 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MT1-MMP in 10 µg of HT-1080 cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P50281
Entrez Gene Number:
NP_004986.1
Gene Symbol:
MMP14
MTMMP1
MMP-14
MT1MMP
MT1-MMP
MMP-X1
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Alternate Names:
MT1MMP
MMP-14
MMP14
Membrane-type-1 matrix metalloproteinase
Membrane-type matrix metalloproteinase 1
matrix metalloproteinase 14 (membrane-inserted)
matrix metallopeptidase 14 (membrane-inserted)
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Usage Statement:
Unless otherwise stated in our catalog or other co 2-8℃