SUMO-1-AMC
货号： BML-UW0040-0025 产品名称： SUMO-1-AMC 品牌： Biomol 规格： 25 ug 3周到货 生化实验
SUMO-1-AMC
Fluorogenic substrate for deSUMOylating enzymes. SUMO1-AMC is a sensitive fluorogenic substrate for deSUMOylating enzymes, including SENP1 (Prod. No. BML-UW9760) and SENP2 (Prod. No. BML-UW9765). It is a particularly useful reagent for the study of deSUMOylating activity where detection sensitivity or continuous monitoring of activity is essential.
Product Specification
Alternative Name: Sentrin-1-AMC
MW: ~11.3kDa
Source: Produced in E. coli.
UniProt ID: P63165
Formulation: Liquid. In DMSO.
Purity: ≥95% (HPLC)
Application: Uses:
1. Substrate for deSUMOylating enzyme activity assays.
2. Identification/confirmation of enzyme deSUMOylation activity.
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC substrates (e.g. Ubiquitin-AMC, Prod. No. BML-SE211).
Short Term Storage: -80°C
Long Term Storage: -80°C
Use/Stability: Stable for at least 12 months after receipt when stored at -80°C.
Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
-80°C
BML-UW0045-0025 SUMO-2-AMC 品牌 Biomol
SUMO-2-AMC
货号： BML-UW0045-0025 产品名称： SUMO-2-AMC 品牌： Biomol 规格： 25 ug 3周到货 生化实验
SUMO-2-AMC
Fluorogenic substrate for deSUMOylating enzymes.SUMO-2-AMC is a sensitive fluorogenic substrate for deSUMOylating enzymes, including SENP1 (Prod. No. BML-UW9760) and SENP2 (Prod. No. BML-UW9765). It is a particularly useful reagent for the study of deSUMOylating activity where detection sensitivity or continuous monitoring of activity is essential.
Product Specification
Alternative Name: Sentrin-2-AMC
MW: ~10.8kDa
Source: Produced in E. coli.
UniProt ID: P61956
Formulation: Liquid. In DMSO.
Purity: ≥95% (HPLC)
Application: Uses:
1. Substrate for deSUMOylating enzyme activity assays.
2. Identification/confirmation of enzyme deSUMOylation activity.
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC substrates (e.g. Ubiquitin-AMC, Prod. No. BML-SE211).
Short Term Storage: -80°C
Long Term Storage: -80°C
Use/Stability: Stable for at least 12 months after receipt when stored at -80°C.
Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
-80°C
BML-UW0050-0025 NEDD8-AMC 品牌 Biomol
NEDD8-AMC
货号： BML-UW0050-0025 产品名称： NEDD8-AMC 品牌： Biomol 规格： 25 ug 3周到货 生化实验
NEDD8-AMC
NEDD8-AMC is a fluorogenic substrate for deNEDDylating enzymes, including NEDP1 and UCH-L3. It is a particularly useful reagent for the study of deNEDDylating activity where detection sensitivity or continuous monitoring of activity is essential.
Product Specification
MW: 8.735 kDa
Concentration: 1 mg/ml
Formulation: Solution in DMSO.
Purity: ≥95% (HPLC)
Appearance: Liquid.
Application: 1. Substrate for deNEDDylating enzyme activity assays.
2. Identification/confirmation of enzyme deNEDDylation activity.
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC substrates (e.g. Ubiquitin-AMC, Prod. No. BML-SE211).
Long Term Storage: -80°C
-80°C
BML-UW0060-0025 SUMO-1 Aldehyde 品牌 Biomol
SUMO-1 Aldehyde
货号： BML-UW0060-0025 产品名称： SUMO-1 Aldehyde 品牌： Biomol 规格： 25 ug 3周到货 生化实验
SUMO-1 aldehyde
Inhibitor of deSUMOylating enzymes (SENPs). SUMO-1 aldehyde is a highly specific inhibitor of SENP enzymes and has utility in the stabilisation of SUMO-protein conjugates in vitro, enhancing their accumulation in cell lysates and tissue extracts.
Product Specification
Alternative Name: Sentrin-1 aldehyde
MW: ~11.1kDa
Source: Produced in E. coli cells. Derivatised chemically, in vitro.
UniProt ID: P63165
Concentration: 1mg/ml
Formulation: Liquid. In 0.15M HCl.
Purity: ≥95% (HPLC)
Short Term Storage: +4°C
Long Term Storage: +4°C
Use/Stability: Soluble and stable in aqueous solution at pH ≤7.0. Avoid presence of amino-containing compounds. Do not lyophilise. Do not neutralise until immediately prior to use.Stable for at least 2 months after receipt when stored at +°C.
Handling: Do not freeze.
+4°C
BML-UW0065-0025 SUMO-2 Aldehyde 品牌 Biomol
SUMO-2 Aldehyde
货号： BML-UW0065-0025 产品名称： SUMO-2 Aldehyde 品牌： Biomol 规格： 25 ug 3周到货 生化实验
SUMO-2 aldehyde
Inhibitor of deSUMOylating enzymes (SENPs). SUMO-2 aldehyde is a highly specific inhibitor of SENP enzymes and has utility in the stabilisation of SUMO-protein conjugates in vitro, enhancing their accumulation in cell lysates and tissue extracts.
Product Specification
Alternative Name: Sentrin-2 aldehyde
MW: ~10.5kDa
Source: Produced in E. coli cells. Derivatised chemically, in vitro.
UniProt ID: P55854
Concentration: 1mg/ml
Formulation: Liquid. In 0.15M HCl.
Purity: ≥95% (HPLC)
Short Term Storage: +4°C
Long Term Storage: +4°C
Use/Stability: Soluble and stable in aqueous solution at pH ≤7.0. Avoid presence of amino-containing compounds. Do not lyophilize. Do not neutralize until immediately prior to use. Stable for at least 2 months after receipt when stored at +4°C.
Handling: Do not freeze.
+4°C
BML-UW0070-0050 NEDD8 Aldehyde 品牌 Biomol
NEDD8 Aldehyde
货号： BML-UW0070-0050 产品名称： NEDD8 Aldehyde 品牌： Biomol 规格： 50 ug 3周到货 生化实验
NEDD8 aldehyde
NEDD8-aldehyde is a potent, highly specific, reversible inhibitor of deNEDDylating and NEDD8 processing enzymes, including NEDP1 (aka DEN1/SENP8) and UCH-L3.
Product Specification
Application: Stabilization of NEDD8-protein conjugates in cell lysates and tissue extracts. Identification and confirmation of enzyme NEDD8 protease activity. Determination of inhibition kinetics for specific deNEDD8ylating enzymes. Co-crystallization with specific deNEDDylating enzymes for structural studies.
Long Term Storage: -20°C
-20°C
BML-UW0095-0500 SUMO-1 (human) (recombinant) (Agarose Immobilized) 品牌 Biomol
SUMO-1 (human) (recombinant) (Agarose Immobilized)
货号： BML-UW0095-0500 产品名称： SUMO-1 (human) (recombinant) (Agarose Immobilized) 品牌： Biomol 规格： 0.5 mL 3周到货 生化实验
SUMO-1 (human), (recombinant) (agarose immobilized)
For isolation and enrichment of SUMO-1 interacting proteins. SUMO-1 agarose may have considerable utility for the capture/pull down of SUMO-1 interacting partners, including processing/signalling pathway proteins and SUMO-1 modification substrates, from cell lysates and extracts by affinity binding. The immobilized protein retains the exposed C-terminal diglycine required for SUMO-1 modification and therefore may be suitable for in vitro SUMOylation. Captured proteins can be eluted readily for identification, analysis or subsequent biochemical reaction.
Product Specification
Alternative Name: Sentrin-1, Small ubiquitin-related modifier 1
Source: Human recombinant SUMO-1 coupled to agarose at 0.5mg protein per ml settled resin.
UniProt ID: P63165
Application: Capture and analysis of SUMO-1 interacting proteins from cell/tissue lysates. Isolation of specific SUMO-1 interacting proteins for use in subsequent biochemical experiments. Identification of novel SUMO-1 interacting partners. Identification of potential SUMOylation substrates through covalent addition to SUMO-1 target proteins.
Long Term Storage: +4°C
+4°C
BML-UW0100-0500 SUMO-2 (human) (recombinant) (Agarose Immobilized) 品牌 Biomol
SUMO-2 (human) (recombinant) (Agarose Immobilized)
货号： BML-UW0100-0500 产品名称： SUMO-2 (human) (recombinant) (Agarose Immobilized) 品牌： Biomol 规格： 0.5 mL 3周到货 生化实验
SUMO-2 (human), (recombinant) (agarose immobilized)
For isolation and enrichment of SUMO-2 interacting proteins. SUMO-2 agarose may have considerable utility for the capture/pull down of SUMO-2 interacting partners, including processing/signalling pathway proteins and SUMO-2 modification substrates, from cell lysates and extracts by affinity binding. The immobilized protein retains the exposed C-terminal diglycine required for SUMO-2 modification and therefore may be suitable for in vitro SUMOylation. Captured proteins can be eluted readily for identification, analysis or subsequent biochemical reaction.
Product Specification
Alternative Name: Sentrin-2, Small ubiquitin-related modifier 2, SUMO-3
Source: Human recombinant SUMO-2 coupled to agarose at 0.5mg protein per ml settled resin.
UniProt ID: P61956
Application: Capture and analysis of SUMO-2 interacting proteins from cell/tissue lysates. Isolation of specific SUMO-2 interacting proteins for use in subsequent biochemical experiments. Identification of novel SUMO-2 interacting partners. Identification of potential SUMOylation substrates through covalent addition to SUMO-2 target proteins.
Long Term Storage: +4°C
+4°C
BML-UW0105-0500 SUMO-3 (human) (recombinant) (Agarose Immobilized) 品牌 Biomol
SUMO-3 (human) (recombinant) (Agarose Immobilized)
货号： BML-UW0105-0500 产品名称： SUMO-3 (human) (recombinant) (Agarose Immobilized) 品牌： Biomol 规格： 0.5 mL 3周到货 生化实验
SUMO-3 (human), (recombinant) (agarose immobilized)
For isolation and enrichment of SUMO-3 interacting proteins. SUMO-3 agarose may have considerable utility for the capture/pull down of SUMO-3 interacting partners, including processing/signalling pathway proteins and SUMO-3 modification substrates, from cell lysates and extracts by affinity binding. The immobilized protein retains the exposed C-terminal diglycine required for SUMO-3 modification and therefore may be suitable for in vitro SUMOylation. Captured proteins can be eluted readily for identification, analysis or subsequent biochemical reaction.
Product Specification
Alternative Name: Sentrin-3, Small ubiquitin-related modifier 3, SUMO-2
Source: Human recombinant SUMO-3 coupled to agarose at 0.5mg protein per ml settled resin.
UniProt ID: P55854
Application: Capture and analysis of SUMO-3 interacting proteins from cell/tissue lysates. Isolation of specific SUMO-3 interacting proteins for use in subsequent biochemical experiments. Identification of novel SUMO-3 interacting partners. Identification of potential SUMOylation substrates through covalent addition to SUMO-3 target proteins.
Long Term Storage: +4°C
+4°C
BML-UW0110-0500 NEDD8 (human) (recombinant) (Agarose Immobilized) 品牌 Biomol
NEDD8 (human) (recombinant) (Agarose Immobilized)
货号： BML-UW0110-0500 产品名称： NEDD8 (human) (recombinant) (Agarose Immobilized) 品牌： Biomol 规格： 0.5 mL 3周到货 生化实验
NEDD8 (human), (recombinant) (agarose immobilized)
For isolation and enrichment of NEDD8 interacting proteins. NEDD8 agarose may have considerable utility for the capture/pull down of NEDD8 interacting partners, including processing/signalling pathway proteins and NEDD8 modification substrates, from cell lysates and extracts by affinity binding. The immobilized protein retains the exposed C-terminal diglycine required for NEDD8 modification and therefore may be suitable for in vitro NEDDylation. Captured proteins can be eluted readily for identification, analysis or subsequent biochemical reaction.
Product Specification
Source: Human recombinant NEDD8 coupled to agarose at 0.5mg protein per ml settled resin.
UniProt ID: Q15843
Application: Capture and analysis of NEDD8 interacting proteins from cell/tissue lysates. Isolation of specific NEDD8 interacting proteins for use in subsequent biochemical experiments. Identification of novel NEDD8 interacting partners. Identification of potential NEDDylation substrates through covalent addition to NEDD8 target proteins.
Long Term Storage: +4°C
+4°C
BML-UW0115-0500 ISG15 (human) (recombinant) (Agarose Immobilized) 品牌 Biomol
ISG15 (human) (recombinant) (Agarose Immobilized)
货号： BML-UW0115-0500 产品名称： ISG15 (human) (recombinant) (Agarose Immobilized) 品牌： Biomol 规格： 0.5 mL 3周到货 生化实验
ISG15 (human), (recombinant) (agarose immobilized)
For isolation and enrichment of ISG15 interacting proteins. ISG15 agarose may have considerable utility for the capture/pull down of ISG15 interacting partners, including processing/signalling pathway proteins and ISG15 modification substrates, from cell lysates and extracts by affinity binding. The immobilized protein retains the exposed C-terminal diglycine required for ISG15 modification and therefore may be suitable for in vitro ISGylation. Captured proteins can be eluted readily for identification, analysis or subsequent biochemical reaction.
Product Specification
Source: Human recombinant ISG15 coupled to agarose at 0.5mg protein per ml settled resin.
UniProt ID: P05161
Application: Capture and analysis of ISG15 interacting proteins from cell/tissue lysates. Isolation of specific ISG15 interacting proteins for use in subsequent biochemical experiments.Identification of novel ISG15 interacting partners.Identification of potential ISGylation substrates through covalent addition to ISG15 target proteins.
Long Term Storage: +4°C
+4°C
BML-UW0120-0001 Ubiquitin Binding Entities Sampler Pack 品牌 Biomol
Ubiquitin Binding Entities Sampler Pack
货号： BML-UW0120-0001 产品名称： Ubiquitin Binding Entities Sampler Pack 品牌： Biomol 规格： Pack 3周到货 生化实验
Ubiquitin binding entities, sampler pack
The UBE Sampler Kit provides a range of UBE agarose conjugates for use in such experiments and for investigation of UBE:Ubiquitin interactions.
Product Specification
Application: Isolation and detection of the full range of ubiquitinylated protein conjugates from a specific cell/tissue lysate. Capture and analysis of specific ubiquitinylated protein conjugates of interest from particular cell/tissue lysates. Pull down of ubiquitinylated proteins from in vitro assays facilitating their semi-quantitative analysis. Separation of ubiquitinylated/non-ubiquitinylated forms of specific proteins of interest. Release of free proteins in their active/native form by cleavage of ubiquitin/ubiquitin chains from the matrix using a deubiquitinylating enzyme. Release of ubiquitinylated proteins in their active/native form by elution from the matrix using high salt buffer.
Long Term Storage: +4°C
+4°C
BML-UW0140-0500 FAT10 (human) (recombinant) (Agarose Immobilized) 品牌 Biomol
FAT10 (human) (recombinant) (Agarose Immobilized)
货号： BML-UW0140-0500 产品名称： FAT10 (human) (recombinant) (Agarose Immobilized) 品牌： Biomol 规格： 0.5 mL 3周到货 生化实验
FAT10 (human), (recombinant) (agarose immobilized)
For the isolation and enrichment of FAT10 interacting proteins. FAT10 agarose conjugate may have potential utility for the capture/pull down of FAT10-interacting partners, including processing/signaling pathway proteins and FAT10 target proteins, from cell lysates and extracts by affinity binding. The immobilized protein retains the exposed C-terminal diglycine required for FAT10 modification and therefore may be suitable for in vitro FAT10 conjugation. It is anticipated that captured proteins may be eluted for identification, analysis or subsequent biochemical reaction.
Product Specification
Source: Human recombinant FAT10 coupled to agarose at 0.5mg protein per ml settled resin.
UniProt ID: O15205
Application: Capture and analysis of FAT10-interacting proteins from cell/tissue lysates. Isolation of specific FAT10-interacting proteins for use in subsequent biochemical experiments.Identification of novel FAT10-interacting partners.Identification of potential FAT10 substrates through covalent addition to FAT10 target proteins.
Long Term Storage: +4°C
+4°C
BML-UW0155-0025 Ubiquitin Vinyl Sulfone (HA-tagged) 品牌 Biomol
Ubiquitin Vinyl Sulfone (HA-tagged)
货号： BML-UW0155-0025 产品名称： Ubiquitin Vinyl Sulfone (HA-tagged) 品牌： Biomol 规格： 25 ug 3周到货 生化实验
Ubiquitin vinyl sulfone, (HA-tag)
For use in the detection and identification of deubiquitinylating enzymes. HA-Ub-VS is a DUB active site directed probe generated by a chemical ligation method, that acts as a potent and irreversible inhibitor of DUBs through covalent modification of the active site and as a specific probe for enzymes with DUB activity. The HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein, facilitates sensitive identification or purification of HA-Ub-VS modified DUBs through recognition by anti-HA antibodies and/or anti-HA-agarose.
Product Specification
Alternative Name: HA-Ub-VS
MW: ~9.8kDa
Source: Synthetic.
UniProt ID: P62988
Formulation: Lyophilized.
Purity: ≥95% (SDS-PAGE)
Application: Suggested uses:
1. Deubiquitinylating enzyme active site-directed probes for detection of novel DUBs.
2. Determination of deubiquitinylating enzyme activity of putative DUBs.
3. Identification of DUBs from biological samples via immunoprecipitation and proteomic methods.
4. Characterisation of deconjugating enzymes for Ub/Ubl specificity.
Reconstitution: Reconstitute with aqueous buffer up to 5mg/ml (~500µM).
Short Term Storage: -80°C
Long Term Storage: -80°C
Use/Stability: Stable for at least 6 months after receipt when stored at -80°C. Once reconstituted store HA-Ub-VS solution at -80ºC, avoid multiple freeze/thaw cycles and use within 1 month.
Handling: Avoid freeze/thaw cycles.
-80°C
BML-UW0170-0500 SUMO-3 (human) (recombinant) (GST) 品牌 Biomol
SUMO-3 (human) (recombinant) (GST)
货号： BML-UW0170-0500 产品名称： SUMO-3 (human) (recombinant) (GST) 品牌： Biomol 规格： 500 ug 3周到货 生化实验
SUMO-3 (human) (recombinant) (GST-tag)
Product Specification
Alternative Name: Sentrin-3, Small ubiquitin-related modifier 3, SUMO-2
MW: 36.8 kDa
Source: Produced in E. coli. Human SUMO-3 is fused to a GST-tag.
UniProt ID: P55854
Formulation: Liquid. In TBS and 1 mM DTT.
Purity: ≥95% (SDS-PAGE)
Purity Detail: Purified by glutathione affinity chromatography.
Application: Mature protein for functional studies
Long Term Storage: -80°C
Miscellaneous/General: Conjugation of mature SUMO proteins to specific lysine residues on target proteins via isopeptide bonds, is involved in a range of processes including nuclear protein targeting, formation of sub-nuclear complexes, regulation of transcriptional activities, and control of protein stability.
-80°C
BML-UW0175-0100 (E93R)SUMO-1 (human) (recombinant) (GST) 品牌 Biomol
(E93R)SUMO-1 (human) (recombinant) (GST)
货号： BML-UW0175-0100 产品名称： (E93R)SUMO-1 (human) (recombinant) (GST) 品牌： Biomol 规格： 100 ug 3周到货 生化实验
[E93R]SUMO-1 (human), (recombinant) (GST-tag)
C-Terminal mutant of mature SUMO-1 protein for use in proteomic investigations. The use of this mutant SUMO-1 for the modification of target proteins, with recovery of resulting SUMO conjugates using the GST-tag, followed by tryptic digestion gives rise to peptides with a SUMO specific side chain signature not readily obtainable using wild-type SUMO modification. This modification allows ready differentiation by mass spectrometric analysis of SUMO modifications from those derived from ubiquitin and other Ubls.
Product Specification
UniProt ID: P63165
Application: Identification of SUMO modified proteins by proteomic methods. Identification of SUMO modification sites by proteomic methods.
Long Term Storage: -80°C
-80°C
BML-UW0200-0025 Hdm2 (human) (recombinant) (Catalytic RING Domain) (GST) 品牌 Biomol
Hdm2 (human) (recombinant) (Catalytic RING Domain) (GST)
货号： BML-UW0200-0025 产品名称： Hdm2 (human) (recombinant) (Catalytic RING Domain) (GST) 品牌： Biomol 规格： 25 ug 3周到货 生化实验
Hdm2 (catalytic RING domain) (human), (recombinant) (GST-tag)
p53 is a much studied and complex multifunctional protein, which plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis, through its involvement in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for these processes. Activation and regulation of the p53 transcription pathway is controlled by a range of post-translational modifications including acetylation, phosphorylation and ubiquitinylation. In normal cells, p53 is maintained at a low level mainly through Hdm2-mediated ubiquitinylation and subsequent degradation by the proteasome. Hdm2 is a RING domain dependent ubiquitin E3 ligase that utilizes its C-terminal RING domain to promote not only p53 ubiquitinylation, predominantly at the C-terminus of p53, but also to target Hdm2 itself for auto-ubiquitinylation and subsequent degradation. The isolated Hdm2 C-terminal RING domain (residues 418-491) has been shown to be sufficient for both p53 and self-ubiquitinylation activity.
Product Specification
Alternative Name: Human double minute 2 protein
MW: ~35kDa
Source: Produced in E. coli.
UniProt ID: Q00987
Formulation: Liquid. In TBS, pH 7.5, containing 150mM sodium chloride and 1mM DTT.
Purity: ≥95% (SDS-PAGE)
Purity Detail: Purified by glutathione affinity chromatography.
Activity: Modifies p53 (Prod. No. BML-FW8820) in ubiquitinylation reactions containing Ube1 (Prod. No. BML-UW9410), UbcH5b (Prod. No. BML-UW9060) and energy regeneration solution.
Application: 1.in vitro generation of ubiquitinylated p53 for use in Ub-p53 related studies.
2.Investigation of the p53 ubiquitin modification process itself.
3.Ubiquitin modification of other known Hdm2 substrate proteins.
4.NEDD8 modification of p53.
Long Term Storage: -80°C
-80°C
BML-UW0205-1000 (KallR)Ubiquitin (Untagged) (human) (recombinant) 品牌 Biomol
(KallR)Ubiquitin (Untagged) (human) (recombinant)
货号： BML-UW0205-1000 产品名称： (KallR)Ubiquitin (Untagged) (human) (recombinant) 品牌： Biomol 规格： 1 mg 3周到货 生化实验
[KallR]Ubiquitin (human), (recombinant) (untagged)
Product Specification
Sequence: H-MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQ RLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG-OH
MW: ~8.7 kDa
Source: Produced in E. coli.
UniProt ID: P62988
Formulation: Lyophilized.
Purity: ≥95% (SDS-PAGE) (Coomassie staining)
Purity Detail: Purified by perchloric acid treatment of a soluble lysate, followed by gradient elution from a cation exchange column.
Appearance: White solid.
Reconstitution: Reconstitute with aqueous buffers or DMSO.
Short Term Storage: -20°C
Long Term Storage: -20°C
Use/Stability: Store solid at –20°C for up to twelve months. Store solutions at –20°C for up to three months.
-20°C
BML-UW0210-0001 K6-only Ubiquitin (Untagged) (human) (recombinant) 品牌 Biomol
K6-only Ubiquitin (Untagged) (human) (recombinant)
货号： BML-UW0210-0001 产品名称： K6-only Ubiquitin (Untagged) (human) (recombinant) 品牌： Biomol 规格： 1 mg 3周到货 生化实验
K6-only Ubiquitin (human), (recombinant) (untagged)
For production of poly-ubiquitin chains via Lys6 only. Lys6-only ubiquitin contains only a single lysine at position 6, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains only via Lys6. The ability to form an active thioester at the C-terminus is preserved, allowing Lys6-only ubiquitin to be transferred to the lysines of substrate proteins.
Product Specification
MW: ~8.6 kDa
Source: Produced in E. coli.
UniProt ID: P0CG48
Formulation: Lyophilized.
Purity: ≥95% (SDS-PAGE) (SDS-PAGE)
Purity Detail: Purified by perchloric acid treatment of a soluble lysate, followed by gradient elution from a cation exchange column.
Appearance: White solid.
Application: 1. Production of Lys6-linked poly-ubiquitin chain conjugated proteins.
2. Investigation of the formation and/or function of specific proteins modified by Lys6-linked poly-ubiquitin chains.
3. Study of the production and/or role of poly-ubiquitin chains linked viaLys6 only.
Long Term Storage: -20°C
-20°C
BML-UW0215-0001 K11-only Ubiquitin (Untagged) (human) (recombinant) 品牌 Biomol
K11-only Ubiquitin (Untagged) (human) (recombinant)
货号： BML-UW0215-0001 产品名称： K11-only Ubiquitin (Untagged) (human) (recombinant) 品牌： Biomol 规格： 1 mg 3周到货 生化实验
K11-only Ubiquitin (human), (recombinant) (untagged)
Product Specification
MW: ~8.6 kDa
Source: Produced in E. coli.
UniProt ID: P0CG48
Formulation: Lyophilized.
Purity: ≥95% (SDS-PAGE) (SDS-PAGE)
Purity Detail: Purified by perchloric acid treatment of a soluble lysate, followed by gradient elution from a cation exchange column.
Appearance: White solid.
Application: For production of poly-ubiquitin chains via Lys11 only.
Long Term Storage: -20°C
Miscellaneous/General: Lys11-only ubiquitin contains only a single lysine at position 11, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form multi-ubiquitin chains only via Lys11. The ability to form an active thioester at the C-terminus is preserved, allowing Lys11-only ubiquitin to be transferred to the lysines of substrate proteins.
-20°C