PicoProbe Glucose-6-Phosphate Assay Kit

货号： K687-100      产品名称： PicoProbe Glucose-6-Phosphate Assay Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

PicoProbe™ Glucose-6-Phosphate Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium etc.
• Species reactivity- Mammalian
• Applications- Applications- The kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample, in a high-throughput screening (HTS) format.
Features & Benefits:
• Simple procedure; takes ~ 15-20 minutes to complete the assay
• Fast and convenient
• Sensitive, efficient and accurate assay
Kit Components:
• G6P Assay Buffer
• PicoProbe™ (in DMSO)
• G6P Enzyme Mix
• G6P Substrate Mix
• G6PStandard (10 µmol)
Description:
Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. BioVision’s glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). The Glucose-6-phosphate Assay Kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. The PicoProbe™ G6P Assay Kit is much more sensitive than the G6P Colorimetric Assay Kit for low G6P samples
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

PicoProbe Fructose-6-Phosphate Assay Kit

货号： K689-100      产品名称： PicoProbe Fructose-6-Phosphate Assay Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

PicoProbe™ Fructose-6-Phosphate Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth media and food products
• Species reactivity- Mammalian
• Applications- The PicoProbe™ F6P Assay Kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity ~1 µM of F6P
Features & Benefits:
• Simple procedure; takes ~ 15-20 minutes to complete the assay
• Fast and convenient
• Sensitive, efficient and accurate assay
Kit Components:
• F6P Assay Buffer
• F6P Probe
• F6P Enzyme Mix
• F6P Converter
• F6P Developer
• F6P Standard (10 µmol)
Description:
Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator (1). BioVision’s PicoProbe ™ Fructose-6-phosphate Assay Kit is a fluorescence-based simple, highly sensitive and rapid means of quantifying F6P in a variety of samples. In the assay, F6P is converted to glucose-6-phosphate which is subsequently oxidized with the generation of a fluorescent product. The PicoProbe™ F6P Assay Kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity ~1 µM of F6P.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

PhosphoSeek P I3-Kinase Assay Kit

货号： K706-400      产品名称： PhosphoSeek P I3-Kinase Assay Kit   品牌： Biovision 规格： 400 assays 3周到货 生化实验

PhosphoSeek™ P I3-Kinase Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Fluorescence (Ex/Em 540/580 nm)
• Sample type- Purified/ Isolated enzyme; PI3-K Inhibitor Candidates
• Species reactivity- Mammalian
• Applications- Measure enzyme activity from purified enzyme preparation, screen  PI3-K  Inhibitor Candidates  using purified/ recombinant enzymes
Features & Benefits:
• Simple procedure
• Fast and convenient
Kit components:
• Assay Buffer
• Lipid Substrate
• Calibrator
• Post Reaction Buffer
• Sensor Stock
• Sensor Dilution Buffer
• ATP
• DTT
• 384 well plate
Description:
The PhosphoSeek™ Technology for screening of kinase and phosphatase activities is a robust and homogeneous detection platform that measures the activity of a target enzyme. Assays are non-competitive with respect to substrate and do not require radioactive materials or secondary (detector) enzymes or antibodies. These biochemical assays are ideally suited for automated screening and can be read on any fluorometer. The Sensor is a proprietary fluorescent molecule that contains a trivalent metal ion, which binds to phosphorylated biological substrates. Phosphorylation is measured by the change of fluorescence of a dye-labeled and phosphorylated substrate when bound by the Sensor. The change in fluorescence directly correlates to the level of substrate conversion.
Storage Conditions:
MT
Shipping Conditions:
USAGE: For Research Use Only! Not For Use in Humans.

-20°C