PhosphoSeek P I3-Kinase Assay Kit

货号： K706-400      产品名称： PhosphoSeek P I3-Kinase Assay Kit   品牌： Biovision 规格： 400 assays 3周到货 生化实验

PhosphoSeek™ P I3-Kinase Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Fluorescence (Ex/Em 540/580 nm)
• Sample type- Purified/ Isolated enzyme; PI3-K Inhibitor Candidates
• Species reactivity- Mammalian
• Applications- Measure enzyme activity from purified enzyme preparation, screen  PI3-K  Inhibitor Candidates  using purified/ recombinant enzymes
Features & Benefits:
• Simple procedure
• Fast and convenient
Kit components:
• Assay Buffer
• Lipid Substrate
• Calibrator
• Post Reaction Buffer
• Sensor Stock
• Sensor Dilution Buffer
• ATP
• DTT
• 384 well plate
Description:
The PhosphoSeek™ Technology for screening of kinase and phosphatase activities is a robust and homogeneous detection platform that measures the activity of a target enzyme. Assays are non-competitive with respect to substrate and do not require radioactive materials or secondary (detector) enzymes or antibodies. These biochemical assays are ideally suited for automated screening and can be read on any fluorometer. The Sensor is a proprietary fluorescent molecule that contains a trivalent metal ion, which binds to phosphorylated biological substrates. Phosphorylation is measured by the change of fluorescence of a dye-labeled and phosphorylated substrate when bound by the Sensor. The change in fluorescence directly correlates to the level of substrate conversion.
Storage Conditions:
MT
Shipping Conditions:
USAGE: For Research Use Only! Not For Use in Humans.

-20°C