Aconitase Activity Assay Kit
货号: AAMT009-1KIT 产品名称: Aconitase Activity Assay Kit 品牌: Merck 规格: *** 3周到货 生化实验
Aconitase Activity Assay Kit
Product Description
The active form of the enzyme is inhibited by citrate analogs, and fluoracetate. Other inhibitors include oxidative stress agents such as peroxynitrite, hydrogen peroxide and superoxide, which inactivate the enzyme by changing the [4Fe-4S] to a [3Fe-4S] cluster. Therefore, aconitase is considered a good marker of mitochondrial and cellular oxidative stress. This inhibition of mitochondrial aconitase can lead to a decreased energy production, whereas in cytosolic aconitase it triggers binding of the enzyme to mRNA iron response elements resulting in increased expression of iron uptake proteins and decreased transcription of iron sequestering protein.
A hydroxyl scavenging solution (Aconitase preservation solution) is supplied with this kit to maintain aconitase activity during sample preparation. An inactivated [3Fe-4FS] aconitase may be activated in vitro by the addition of iron and cysteine.
Citrate ←→ cis-aconitate ←→ isocitrate
(↑ Abs. 240 nm)
The Aconitase Activity Assay Kit measures the activity of aconitase in a sample by following the conversion of isocitrate to cis-aconitate as indicated by an increase in absorbance at UV 240 nm with an extinction coefficient of 2.2 OD/mM per well.
Note: Mitochondrial and cytoplasmic aconitase activities are indistinguishable. Therefore, to measure the mitochondrial activity only, first isolate mitochondria, but to measure for both activities, separate the cells into cytoplasmic and mitochondrial fractions (see TB552 for details).
The entire assay can be completed within 2 hours. The intra-assay variation and inter-assay variation with this assay are each less than 10% and 15% respectively.
Panel Components
50 ml Buffer (Tube 1), (72575-50ML)
20 ml Aconitase preservation solution, (72576-20ML)
800 µl 25X Isocitrate, (72577-800UL)
200 µl 100X Manganese, (72578-200UL)
1 ea UV Microplate, (72579-1EA)
1 ml Detergent, (72580-1ML)
* This kit contains sufficient materials for 96 measurements (one 96-well microplate).
Storage: Store all components at 2 to 8°C. Do not freeze.
Note: The 96-well microplate has a monoclonal antibody pre-bound to the wells. This plate can be separated into twelve 8 well strips and thus can be used for up to 12 individual experiments.
商店和运输信息
Storage +2°C to +8°C
Ship Blue Ice Only
2°C – 8°C
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700604-250 Aconitase Standard 品牌 Cayman
Aconitase Standard
货号: 700604-250 产品名称: Aconitase Standard 品牌: Cayman 规格: 250 碌l
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
700601-600 Aconitase Fluorometric Inhibitor 品牌 Cayman
Aconitase Fluorometric Inhibitor
货号: 700601-600 产品名称: Aconitase Fluorometric Inhibitor 品牌: Cayman 规格: 600 碌l
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
700602-1 Aconitase Fluorometric Detector 品牌 Cayman
Aconitase Fluorometric Detector
货号: 700602-1 产品名称: Aconitase Fluorometric Detector 品牌: Cayman 规格: 1 ea
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
700600-96 Aconitase Fluorometric Assay Kit 品牌 Cayman
Aconitase Fluorometric Assay Kit
货号: 700600-96 产品名称: Aconitase Fluorometric Assay Kit 品牌: Cayman 规格: 96 wells
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
700603-1 Aconitase Enzyme Mixture 品牌 Cayman
Aconitase Enzyme Mixture
货号: 700603-1 产品名称: Aconitase Enzyme Mixture 品牌: Cayman 规格: 1 ea
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
K716-100 Aconitase Activity Assay Kit 品牌 Biovision
Aconitase Activity Assay Kit
货号: K716-100 产品名称: Aconitase Activity Assay Kit 品牌: Biovision 规格: 100 assays 3周到货 生化实验
Aconitase Activity Colorimetric Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples etc.
• Species reactivity- Mammalian
Features & Benefits:
• Simple procedure; takes ~ less than 40 minutes
• Fast and convenient
• Sensitive assays for measuring Aconitase activity in various biological samples.
Kit components:
• Assay Buffer
• Substrate
• Developer
• Enzyme Mix (200µl)
• Cysteine
• (NH₄)₂Fe(SO₄)₂
• Isocitrate Standard (10µmol)
Description:
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe₄S₄]²ᶧ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.
Storage Conditions:
4°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
+4°C
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)