标签归档：abl

pfuse-mchg2b   pFUSE-CHIg-mG2b   品牌 Invivogen

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pFUSE-CHIg-mG2b

货号： pfuse-mchg2b      产品名称： pFUSE-CHIg-mG2b   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-mG2b
pFUSE-CHIg-mG2b is a cloning plasmid that expresses the constant region of the mouse IgG2b heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-mG2b additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-mG2b features the constant region of the mouse IgG2b heavy chain
• pFUSEss-CHIg-mG2b features the constant region of the mouse IgG2b heavy chain and the IL2 signal sequence
Both plasmids are selectable with Zeocin™.

pfusess-hchg1   pFUSEss-CHIg-hG1   品牌 Invivogen

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pFUSEss-CHIg-hG1

货号： pfusess-hchg1      产品名称： pFUSEss-CHIg-hG1   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1
pFUSE-CHIg-hG1 is a cloning plasmid that expresses the constant region of the human IgG1 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG1 features the constant region of the human IgG1 heavy chain
• pFUSEss-CHIg-hG1 features the constant region of the human IgG1 heavy chain and the IL2 signal sequence
Both plasmids are selectable with Zeocin™.

pfusess-hchg2   pFUSEss-CHIg-hG2   品牌 Invivogen

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pFUSEss-CHIg-hG2

货号： pfusess-hchg2      产品名称： pFUSEss-CHIg-hG2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG2
pFUSE-CHIg-hG2 is a cloning plasmid that expresses the constant region of the human IgG2 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG2 features the constant region of the human IgG2 heavy chain
• pFUSEss-CHIg-hG2 features the constant region of the human IgG2 heavy chain and the IL2 signal sequence
Both plasmids are selectable with Zeocin™.

pfusess-mchg1   pFUSEss-CHIg-mG1   品牌 Invivogen

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pFUSEss-CHIg-mG1

货号： pfusess-mchg1      产品名称： pFUSEss-CHIg-mG1   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-mG1
pFUSE-CHIg-mG1 is a cloning plasmid that expresses the constant region of the mouse IgG1 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-mG1 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-mG1 features the constant region of the mouse IgG1 heavy chain
• pFUSEss-CHIg-mG1 features the constant region of the mouse IgG1 heavy chain and the IL2 signal sequence
Both plasmids are selectable with Zeocin™.

PK1013-50UL   PD ANTI-C-ABL (PTYR245) RABBIT PAB   品牌 Merck

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PD ANTI-C-ABL (PTYR245) RABBIT PAB

货号： PK1013-50UL      产品名称： PD ANTI-C-ABL (PTYR245) RABBIT PAB   品牌： Merck 规格： *** 3周到货 生化实验

PhosphoDetect™ Anti-c-Abl (pTyr245) Rabbit pAb
Anti-p150, Phospho-Specific (Tyr245), Anti-Abl1, Phospho-Specific (Tyr245), Anti-c-Abl, Phospho-Specific (Tyr245)
Recognizes the ~135 kDa c-Abl protein phosphorylated at Tyr245 in HEK293 cells over-expressing constitutively active c-Abl. Also recognizes the ~210 kDa BCR-Abl protein phosphorylated at Tyr245 in K562 cells.
Antibody Target Gene Symbol: ABL1
Target Synonym: ABL, AI325092, bcr/abl, C-ABL, C-ABL 1B, CABL1, E430008G22Rik, JTK7, MGC117749, p145Abl, p150, v-abl
Entrez Gene Name: c-abl oncogene 1, receptor tyrosine kinase
Hu Entrez ID: 25 (Related Antibodies: OP20 PK1006 OP19)
Mu Entrez ID: 11350
Rat Entrez ID: 311860
产品信息
Host  Rabbit
Isotype  IgG
Immunogen  a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr245 phosphorylation site of human c-Abl, coupled to KLH
Form  Liquid
Formulation  In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Species reactivity  human, mouse
Preservative  None
Applications 
Immunoblotting (Western Blotting)
Positive control  HEK293 cells over-expressing constitutively active c-Abl and K562 cells (BCR-Abl)
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Standard Handling

-20°C

V1901   ABL1 Kinase Enzyme System   品牌 Promega

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ABL1 Kinase Enzyme System

货号： V1901      产品名称： ABL1 Kinase Enzyme System   品牌： Promega 规格： 10μg 二周到货 生化试验

说明  
 在 Sf9 昆虫细胞中，我们通过杆状病毒表达 N 端带 His 标签的重组人源 ABL1 （第 27 位氨基酸 – 末端） 。 ABL1 原癌基因编码一个在细胞质和细胞核中都表达的酪氨酸激酶蛋白，该蛋白参与细胞分化、细胞分裂、细胞 粘 附和应激反应等过程。 ABL 蛋白的活性受其自身的 SH3 结构域负调控， SH3 结构域的缺失可使 ABL1 变成癌基因。在 慢性粒细胞白血 病的许多病例中，都出现了 BCR 与 ABL1 基因的转座及头尾融合相接现象。广泛表达的 ABL1 酪氨酸激酶的 DNA 结合活性受 CDK1 介导的磷酸化调控，暗示了 ABL1 具有细胞周期性功能。
ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒） 是一种发光法激酶检测试剂盒，用于 检测 激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶 催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。 该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或ATP 酶) 的活性， ATP 浓度可高达 1 mM 。
激酶酶系统的组成：
•  激酶： ABL1 ， 10μg ( 人源，人工合成， 第 27 位氨基酸 – 末端 ) ； MW : ~135kDa 。
•  底物： Abltide （ EAIYAAPFAKKK ） ；从 Abl 的 C 末端衍生得到。
•  其它组分： 反应缓冲液， DTT 。
ABL1 NCBI 数据库入口 。
人类激酶酶系统及其应用。
特点  •  在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒 与激酶酶系统的优化组合，使您可以迅速方便地开始实验。
•  完整的系统： 激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl 2 。
•  可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。

 

BML-P216-0001   Abl Kinase Peptide Substrate   品牌 Biomol

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Abl Kinase Peptide Substrate

货号： BML-P216-0001      产品名称： Abl Kinase Peptide Substrate   品牌： Biomol 规格： 1 mg* 3周到货 生化实验

This peptide, EAIYAAPFAKKK, was identified as a high affinity (Km=4 µM) and specific substrate for the Abl kinase through a degenerate peptide library. The three lysines on the carboxy-terminus allow this peptide to be used in phosphocellulose kinase assays.
Product Specification
Sequence:Glu-Ala-Ile-Tyr-Ala-Ala-Pro-Phe-Ala-Lys-Lys-Lys
MW:1336.5
Purity:95%
Long Term Storage:-20°C

-20°C

197224-10MG   Bcr-abl Inhibitor III, GNF-5   品牌 Merck

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Bcr-abl Inhibitor III, GNF-5

货号： 197224-10MG      产品名称： Bcr-abl Inhibitor III, GNF-5   品牌： Merck 规格： *** 3周到货 生化实验

Bcr-abl Inhibitor III, GNF-5
N-(2-Hydroxyethyl)-3-(6-(4-(trifluoromethoxy)phenylamino)pyrimidin-4-yl)benzamide, Abl Inhibitor III
A cell-permeable GNF-2 (Cat. No. 197221) N-hydroxyethyl carboxamide analog that exhibits in vivo efficacy in suppressing the proliferation of Bcr-abl-expressing Ba/F3 (93% and 83% of no-treatment control, respectively, on days 5 and 7 post treatment; 100 mg/kg b.i.d.) and bone marrow cells (~75% of no-treatment control in both WBC counts and spleen weight on day 7 post treatment; 50 mg/kg b.i.d.) in murine xenograft models of leukemia. Similar to GNF-2, GNF-5 exerts its effect via an allosteric mechanism (IC50 = 0.22 µM against wild-type Abl) by targeting the myristate-binding pocket near the c-terminus of Abl kinase domain and thereby altering the conformational dynamics of the ATP-binding pocket. GNF-5 is ineffective toward the myristate-binding site mutant E505K and the ATP-binding site ‘gatekeeper’ mutant T315I.
产品信息
Form  Off-white powder
Molar mass  418.4
Protect from Light  是的
Packaged under inert gas  是的
Purity  ≥97% by HPLC
Solubility  DMSO
Chemical formula  C20H17F3N4O3
商店和运输信息
Storage  +2°C to +8°C
Ship  Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling

2°C – 8°C

07-2178   Anti-Abl interactor 1   品牌 Millipore

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Anti-Abl interactor 1

货号： 07-2178      产品名称： Anti-Abl interactor 1   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Abl interactor 1
Description:
Anti-Abl interactor 1 Antibody
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Molecular Weight:
~60 kDa observed. Uniprot describes many isoforms between 43-55 kDa that can be highly phosphorylated.
Immunogen:
KLH-conjugated linear peptide corresponding to human Abl interactor 1.
Background Information:
Abl interactor 1 (Abelson interactor 1) is a member of the family of Abl-interactors and is highly expressed in the brain. Abl interactor 1 is involved in the signaling of ABL1 and ABL2 nonreceptor tyrosine kinases, and the EGF-receptor. The Abl interactor 1 protein contains SH3 domains, and may function as an adaptor protein; It forms a trimeric complex with EPS8 and SOS1 in the transduction of signals from Ras to Rac. Previous studies have suggested that Abl interactor 1 via the Ras and Rac pathways may regulate cell proliferation, cell adhesion, and actin remodeling. Abl interactor 1 is also investigated for its role in tumor suppression.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Demonstrated to react with Human and Mouse. Predicted to react with Rat and Bovine based on 100% sequence homology.
Control:
Jurkat cell lysate
Quality Assurance:
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Abl interactor 1 on 10 µg of Jurkat cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q8IZP0
Entrez Gene Number:
NP_005461
Gene Symbol:
ABI1
SSH3BP1
Alternate Names:
Abl interactor 1
Abelson interactor 1
Abi-1
Abl-binding protein 4
AblBP4
Eps8 SH3 domain-binding protein
Eps8-binding protein
Nap1-binding protein
Nap1BP
Spectrin SH3 domain-binding protein 1
e3B1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Product Name:
Anti-Abl interactor 1
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Affinity Purified
Host:
Rabbit

2°C – 8°C