Anti-RHOC

货号： ABT295      产品名称： Anti-RHOC   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-RHOC
Species Reactivity Key Applications Host Format Antibody Type
H  WB, IHC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-RHOC Antibody
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Molecular Weight:
~22 kDa observed. An uncharacterized band may appear at ~15 kDa in some lysates.
Immunogen:
GST-tagged recombinant protein corresponding to human RHOC.
Background Information:
Rho-related GTP-binding protein RhoC (RHOC) is a member of the Rho family of small GTPases. RHOC regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas, and breast cancer.
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Species Reactivity:
Human
Application Notes:
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RHOC in human heart tissue.
Control:
HepG2 cell lysate
Quality Assurance:
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected RHOC in 10 µg of HepG2 cell lysate.
Purification Method:
Antigen Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide and 50% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P08134
Gene Symbol:
RHOC
ARH9
ARHC
Alternate Names:
Rho-related GTP-binding protein RhoC
Rho cDNA clone 9
h9
RHOC
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Product Name:
Anti-RHOC
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
150 µL
Format:
Affinity Purified
Host:
Rabbit
Applications:
This RHOC antibody is validated for use in WB & IHC for the detection of the RHOC protein.

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