标签归档：pnp

11700   ADP-ribose-pNP   品牌 AAT

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ADP-ribose-pNP

货号： 11700      产品名称： ADP-ribose-pNP   品牌： AAT 规格： 1 mg 二周到货 生化试验

ADP-ribose-pNP
Cat # 11700
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 405 
MW 724.37
Solvent Water
ADP-ribose-pNP is a colorimetric substrate for assessing activity of poly(ADP-ribose)polymerase (PARP) enzymes. The absorbance of released p-nitrophenol is determined at 405 nm, and the slope of the calibration curve is used to convert the absorbencies to moles of product generated. With ADP-ribose-pNP as the colorimetric substrate, PARP-1 was determined to have the largest Km and Vmax values (151 uM and 1.30 nmolmin-1mg-1 respectively) followed by tankyrase-1 (82 uM and 18 pmolmin-1mg-1 respectively) and VPARP (46 uM and 2 pmolmin-1mg-1 respectively). This colorimetric substrate can be used to determine the kinetic parameters for PARP-1, tankyrase-1, and VPARP, and to screen small-molecule inhibitors of PARP-1, tankyrase-1, and VPARP. ADP-ribose-pNP-based continuous assay has considerable advantages over standard discontinuous PARP assays, enabling the high throughput screening of PARP-1, tankyrase-1, and VPARP activities and their inhibitors.
Detailed Information:   Protocol      References      MSDS
Related Products:     Phosphodiesterases 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)