anti-phospho-PKCd (Ser664)

货号： 07-875      产品名称： anti-phospho-PKCd (Ser664)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-PKCδ (Ser664) Antibody
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-PKCδ (Ser664) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phospho-PKCδ (Ser664), weaker with with mouse and rat PKCδ (Ser662) and PKCα (Ser657) and PKCβII (Ser662).
Molecular Weight:
78 kDa
Immunogen:
Peptide corresponding to amino acid region encompassing the human phospho-PKCδ (Ser664).
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Species Reactivity Note:
Predicted to cross-react more weakly with mouse based on sequence homology, but have not been tested.
Application Notes:
Immunoblot Analysis: A 1:1,000 dilution of this lot detected phospho-PKCδ (Ser664) in RIPA lysate of PMA-treated K562 cells.
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Affinity Purfied
Presentation:
100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Storage Conditions:
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Q05655
Entrez Gene Number:
NM_006254.3
NM_212539.1
Gene Symbol:
PRKCD
MGC49908
nPKC-delta
MAY1
Alternate Names:
PRKCD, PKC delta, PKCd, Protein Kinase C, delta
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.
UniProt Summary:
FUNCTION: SwissProt: Q05655 # This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function.
SIZE: 67 -20℃