anti-phospho-PKCb I&II (Thr500)

货号： 07-870      产品名称： anti-phospho-PKCb I&II (Thr500)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-PKCβ I&II (Thr500) Antibody
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-PKCβ I&II (Thr500) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated PKCβ I&II (Thr500). Minor cross reaction can occur with PKCα (Thr497), PKCγ (Thr514), and PKCε (Thr566) dependent upon cell type and treatment.
Molecular Weight:
~80 kDa
Immunogen:
Peptide corresponding to amino acid region encompassing the human phospho-PKCβ I&II (Thr500)
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Species Reactivity Note:
Predicted to cross-react with mouse and rat based sequence homology, but have not been tested.
Application Notes:
Immunoblot Analysis: A 1:1000 dilution of this lot detected PKCβ I&II in RIPA lysates from PMA treated K562 cells.
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Affinity Purfied
Presentation:
100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Storage Conditions:
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P05771
Entrez Gene Number:
NM_002738.5
NM_212535.1
Gene Symbol:
PRKCB1
PRKCB
PRKCB2
MGC41878
PKCB
PKC-B
PKC-beta
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Alternate Names:
PRKCB1, PRKCB2, PKCB, PKC beta, protein kinase C, beta 1&2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
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UniProt Summary:
-20℃

anti-phospho-PKCb I (Thr642)

货号： 07-872      产品名称： anti-phospho-PKCb I (Thr642)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-PKCβ I (Thr642) Antibody
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-PKCβ I (Thr642) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
phosphorylated PKCβ I (Thr642). Does not cross-react with other phsopho-PKC isoforms at equivalent Thr residues.
Molecular Weight:
~80 kDa
Immunogen:
Peptide corresponding to amino acid region encompassing the human and rat phospho-PKCβ I (Thr642)
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Species Reactivity Note:
Predicted to cross-react with rat based on 100% sequence homology, but have not been tested.
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Affinity Purfied
Storage Conditions:
2 years at -20°C
UniProt Number:
P05771
Entrez Gene Number:
NM_002738.5
NM_212535.1
Gene Symbol:
PRKCB1
PRKCB
PRKCB2
MGC41878
PKCB
PKC-B
PKC-beta
View All »
Alternate Names:
PRKCB1, PKCB, PKC beta, protein kinase C, beta 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
View All »
UniProt Summary:
FUNCTION: SwissProt: P05771 # This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May be considered as a novel component of the NF-kappa-B signaling axis responsible for the survival and activation of B-cells after BCR cross-linking (By similarity).
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
SIZE: 671 amino acids; 76869 Da
SUBUNIT: Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity).
PTM: Phosphorylation on Thr-500 of isoform beta-I, within the activation loop, renders it competent to autophospho -20℃