PKCa, Active

货号： 7714-5      产品名称： PKCa, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active PKCa
Phosphorylate a wide variety of protein targets
Product Overview
Alternate Names / Synonyms: pkcalpha, pkc-alpha, pkca, pkc-a, pkc a, Active PKCa, PKCa,PKC alpha, human PKCa, active kinase, active kinases, PKC, recombinant PKCa, recombinant PKC, kinase activity assay, protein kinase
Gene Symbol: PKCA
Accession #: P17252
Gene ID: 5578
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: 103.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 69 nmol phosphate incorporated into CREBtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PKC-α has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Coussens defined a new family of PKC-related genes and termed α, β, and γ (1). Latos-Bielenska refined the assignment of PRKCA1 to 17q22-q23.2 (2). Braz identified PKC-α as a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes in study of knockout mice (3).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C