标签归档：ngf

4303R-100   NGF-beta, human recombinant   品牌 Biovision

发表于由

NGF-beta, human recombinant

货号： 4303R-100      产品名称： NGF-beta, human recombinant   品牌： Biovision 规格： 100 μg 3周到货 生化实验

NGF-beta, human recombinant
A factor critical for the survival & maintenance of sympathetic and sensory neurons
Product Overview
Alternate Names / Synonyms: Human NGF-beta, NGF-beta, NGFbeta, NGF beta, h-NGF-beta, rh-NGF-beta, recombinant human NGF-beta, recombinant NGF-beta, NGF
Gene Symbol: NGF
Accession #: P01138
Gene ID: 4803
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and then lyophilized with no additives.
Molecular Weight: 13.6 kDa
Purity by SDS-PAGE: ≥97%
Purity by HPLC: ≥97%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the ability to stimulate neurite growth in chick E9 DRG cells is <1.0 ng/ml.
Reconstitution Instructions: Reconstituted human NGF-β should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid freeze/thaw cycles.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Nerve growth factor-β (NGF-β) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-β is a dimer, formed by two identical 119 amino acid subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids and having a molecular weight of 16.95 kDa. The protein is purified by proprietary chromatographic techniques.
Handling: Centrifuge the vial prior to opening.Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-20°C

4303R-1000   NGF-beta, human recombinant   品牌 Biovision

发表于由

NGF-beta, human recombinant

货号： 4303R-1000      产品名称： NGF-beta, human recombinant   品牌： Biovision 规格： 1 mg 3周到货 生化实验

NGF-beta, human recombinant
A factor critical for the survival & maintenance of sympathetic and sensory neurons
Product Overview
Alternate Names / Synonyms: Human NGF-beta, NGF-beta, NGFbeta, NGF beta, h-NGF-beta, rh-NGF-beta, recombinant human NGF-beta, recombinant NGF-beta, NGF
Gene Symbol: NGF
Accession #: P01138
Gene ID: 4803
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and then lyophilized with no additives.
Molecular Weight: 13.6 kDa
Purity by SDS-PAGE: ≥97%
Purity by HPLC: ≥97%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the ability to stimulate neurite growth in chick E9 DRG cells is <1.0 ng/ml.
Reconstitution Instructions: Reconstituted human NGF-β should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid freeze/thaw cycles.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Nerve growth factor-β (NGF-β) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-β is a dimer, formed by two identical 119 amino acid subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids and having a molecular weight of 16.95 kDa. The protein is purified by proprietary chromatographic techniques.
Handling: Centrifuge the vial prior to opening.Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-20°C

4303R-20   NGF-beta, human recombinant   品牌 Biovision

发表于由

NGF-beta, human recombinant

货号： 4303R-20      产品名称： NGF-beta, human recombinant   品牌： Biovision 规格： 20 μg 3周到货 生化实验

NGF-beta, human recombinant
A factor critical for the survival & maintenance of sympathetic and sensory neurons
Product Overview
Alternate Names / Synonyms: Human NGF-beta, NGF-beta, NGFbeta, NGF beta, h-NGF-beta, rh-NGF-beta, recombinant human NGF-beta, recombinant NGF-beta, NGF
Gene Symbol: NGF
Accession #: P01138
Gene ID: 4803
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and then lyophilized with no additives.
Molecular Weight: 13.6 kDa
Purity by SDS-PAGE: ≥97%
Purity by HPLC: ≥97%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the ability to stimulate neurite growth in chick E9 DRG cells is <1.0 ng/ml.
Reconstitution Instructions: Reconstituted human NGF-β should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid freeze/thaw cycles.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Nerve growth factor-β (NGF-β) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-β is a dimer, formed by two identical 119 amino acid subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids and having a molecular weight of 16.95 kDa. The protein is purified by proprietary chromatographic techniques.
Handling: Centrifuge the vial prior to opening.Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-20°C

4304-100   NGF-beta, murine recombinant   品牌 Biovision

发表于由

NGF-beta, murine recombinant

货号： 4304-100      产品名称： NGF-beta, murine recombinant   品牌： Biovision 规格： 100 μg 3周到货 生化实验

NGF-beta, murine recombinant
A factor critical for the survival & maintenance of sympathetic and sensory neurons
Product Overview
Alternate Names / Synonyms: Murine NGF-beta, NGF-beta, NGF beta, NGFbeta, m-NGF-beta, rm-NGF-beta, recombinant murine NGF-beta, recombinant NGF-beta, NGF
Gene Symbol: NGF
Accession #: P01139
Gene ID: 18049
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized with no additives
Molecular Weight: 13.5 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: This NGF- β is fully biologically active when compared to standards. The ED₅₀ as measured by the dose-dependent induction of human TF-1 cell proliferation is typically 0.1–1 ng/ml
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-1.0 mg/ml containing 1% HSA or BSA. The solution can be diluted into other aqueous buffers and stored at 4°C for 2-7 days or store at –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Nerve growth factor-β (NGF-β) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active recombinant mouse NGF-β is a homodimer, non-glycosylated chain containing two identical 120 amino acid subunits, and having a molecular weight of 13.5 kDa.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

 

-20°C

4304-5   NGF-beta, murine recombinant   品牌 Biovision

发表于由

NGF-beta, murine recombinant

货号： 4304-5      产品名称： NGF-beta, murine recombinant   品牌： Biovision 规格： 5 μg 3周到货 生化实验

NGF-beta, murine recombinant
A factor critical for the survival & maintenance of sympathetic and sensory neurons
Product Overview
Alternate Names / Synonyms: Murine NGF-beta, NGF-beta, NGF beta, NGFbeta, m-NGF-beta, rm-NGF-beta, recombinant murine NGF-beta, recombinant NGF-beta, NGF
Gene Symbol: NGF
Accession #: P01139
Gene ID: 18049
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized with no additives
Molecular Weight: 13.5 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: This NGF- β is fully biologically active when compared to standards. The ED₅₀ as measured by the dose-dependent induction of human TF-1 cell proliferation is typically 0.1–1 ng/ml
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-1.0 mg/ml containing 1% HSA or BSA. The solution can be diluted into other aqueous buffers and stored at 4°C for 2-7 days or store at –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Nerve growth factor-β (NGF-β) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active recombinant mouse NGF-β is a homodimer, non-glycosylated chain containing two identical 120 amino acid subunits, and having a molecular weight of 13.5 kDa.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

 

-20°C

11050HNAC25   RECOM MS NGF   品牌 Invitrogen

发表于由

RECOM MS NGF

货号： 11050HNAC25      产品名称： RECOM MS NGF   品牌： Invitrogen 规格： 50 UG 三周到货 生化试验

NGF Recombinant Human Protein
Manufactured by Sino Biological (China); Distributed by Life Technologies.
Nerve growth factor (NGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
This recombinant protein was expressed from a DNA sequence encoding the mature form of human NGF (NP_002497.2) (Ser 122-Arg 239).
N-terminal Sequence Analysis: Ser 122
Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is typically 0.4-1.0 ng/mL.
Formulation: Lyophilized in 50 mM NaAc, 150 mM NaCl, pH 5.5, 5% mannitol, 5% trehalose, 0.02% Tween®-80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under
Form: Lyophilized 
Purity: > 98% by SDS-PAGE 
Species: Human 
Expression: Mammalian 
Gene Symbol: NGF 
Gene Aliases: NGF, Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB 
Product Size: 5 x 5 µg
Protein Form: Full Length,
Recombinant 
Protein Family: Growth Factors & Receptors 
Endotoxin Level: < 1 EU/µg
Protein Subtype: NGFs (Nerve Growth Factors) 
Molecular Weight: 13.2 kDa 
Research Category: Development,
Neurobiology,
Immunology 
Protein Tag or Fusion: Untagged 
Validated Application: Assay Standard
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Includes 5 vial of lyophilized protein. Store at -20 °C.

11050HNAC250   RECOM MS NGF   品牌 Invitrogen

发表于由

RECOM MS NGF

货号： 11050HNAC250      产品名称： RECOM MS NGF   品牌： Invitrogen 规格： 250 UG 三周到货 生化试验

NGF Recombinant Human Protein
Manufactured by Sino Biological (China); Distributed by Life Technologies.
Nerve growth factor (NGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
This recombinant protein was expressed from a DNA sequence encoding the mature form of human NGF (NP_002497.2) (Ser 122-Arg 239).
N-terminal Sequence Analysis: Ser 122
Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is typically 0.4-1.0 ng/mL.
Formulation: Lyophilized in 50 mM NaAc, 150 mM NaCl, pH 5.5, 5% mannitol, 5% trehalose, 0.02% Tween®-80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under
Form: Lyophilized 
Purity: > 98% by SDS-PAGE 
Species: Human 
Expression: Mammalian 
Gene Symbol: NGF 
Gene Aliases: NGF, Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB 
Product Size: 5 x 50 µg 
Protein Form: Full Length,
Recombinant 
Protein Family: Growth Factors & Receptors 
Endotoxin Level: < 1 EU/µg 
Protein Subtype: NGFs (Nerve Growth Factors) 
Molecular Weight: 13.2 kDa 
Research Category: Immunology,
Development,
Neurobiology 
Protein Tag or Fusion: Untagged 
Validated Application: Assay Standard 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Includes 5 vial of lyophilized protein. Store at -20 °C.

11050HNAC5   RECOM MS NGF   品牌 Invitrogen

发表于由

RECOM MS NGF

货号： 11050HNAC5      产品名称： RECOM MS NGF   品牌： Invitrogen 规格： 250 UG 三周到货 生化试验

NGF Recombinant Human Protein
Manufactured by Sino Biological (China); Distributed by Life Technologies.
Nerve growth factor (NGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
This recombinant protein was expressed from a DNA sequence encoding the mature form of human NGF (NP_002497.2) (Ser 122-Arg 239).
N-terminal Sequence Analysis: Ser 122
Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is typically 0.4-1.0 ng/mL.
Formulation: Lyophilized in 50 mM NaAc, 150 mM NaCl, pH 5.5, 5% mannitol, 5% trehalose, 0.02% Tween®-80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under
Form: Lyophilized 
Purity: > 98% by SDS-PAGE 
Species: Human 
Expression: Mammalian 
Gene Symbol: NGF 
Gene Aliases: NGF, Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB
Product Size: 5 µg 
Protein Form: Full Length,
Recombinant 
Protein Family: Growth Factors & Receptors 
Endotoxin Level: < 1 EU/µg 
Protein Subtype: NGFs (Nerve Growth Factors) 
Molecular Weight: 13.2 kDa 
Research Category: Immunology,
Development,
Neurobiology 
Protein Tag or Fusion: Untagged 
Validated Application: Assay Standard
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Includes 1 vial of lyophilized protein. Store at -20 °C.

11050HNAC50   RECOM MS NGF   品牌 Invitrogen

发表于由

RECOM MS NGF

货号： 11050HNAC50      产品名称： RECOM MS NGF   品牌： Invitrogen 规格： 50 UG 三周到货 生化试验

NGF Recombinant Human Protein
Manufactured by Sino Biological (China); Distributed by Life Technologies.
Nerve growth factor (NGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
This recombinant protein was expressed from a DNA sequence encoding the mature form of human NGF (NP_002497.2) (Ser 122-Arg 239).
N-terminal Sequence Analysis: Ser 122
Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is typically 0.4-1.0 ng/mL.
Formulation: Lyophilized in 50 mM NaAc, 150 mM NaCl, pH 5.5, 5% mannitol, 5% trehalose, 0.02% Tween®-80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under
Form: Lyophilized 
Purity: > 98% by SDS-PAGE 
Species: Human 
Expression: Mammalian 
Gene Symbol: NGF 
Gene Aliases: NGF, Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB
Product Size: 50 µg 
Protein Form: Full Length,
Recombinant 
Protein Family: Growth Factors & Receptors 
Endotoxin Level: < 1 EU/µg 
Protein Subtype: NGFs (Nerve Growth Factors)
Molecular Weight: 13.2 kDa 
Research Category: Neurobiology,
Development,
Immunology 
Protein Tag or Fusion: Untagged 
Validated Application: Assay Standard 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Includes 1 vial of lyophilized protein. Store at -20 °C.

01-170   7S NGF, MOUSE 100UG   品牌 Millipore

发表于由

7S NGF, MOUSE 100UG

货号： 01-170      产品名称： 7S NGF, MOUSE 100UG   品牌： Millipore 规格： EA 3周到货 生化实验

NGF 7S Protein, mouse
Description:
NGF 7S, mouse
Trade Name:
Upstate (Millipore)
Qty/Pk:
100 µg
Key Applications:
Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene’s expression is associated with allergic rhinitis.
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UniProt Summary:
FUNCTION: SwissProt: P01138 # Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
SIZE: 241 amino acids; 26959 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
DISEASE: SwissProt: P01138 # Defects in NGFB are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) [MIM:608654]. Hereditary sensory and autonomic neuropathies are characterized by loss of pain sensation in combination with other sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self- mutilation. The autonomic involvement is variable.
SIMILARITY: SwissProt: P01138 ## Belongs to the NGF-beta family.
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Species:
Mouse
Physical Form:
Lyophilized
Brand Family:
Upstate
Source:
Male mouse submaxillary glands
UniProt Number:
P01138
Gene Symbol:
NGFB
HSAN5
NGF
Beta-NGF
MGC161426
MGC161428
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Product Name:
NGF 7S, mouse
Entrez Gene Number:
NM_002506.2
Purification Method:
Sterilized through a 0.2 micron membrane filter

04-1111   Anti-NGF Receptor, clone EP1039Y   品牌 Millipore

发表于由

Anti-NGF Receptor, clone EP1039Y

货号： 04-1111      产品名称： Anti-NGF Receptor, clone EP1039Y   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-NGF Receptor, clone EP1039Y, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  IH(P), WB, ICC, IP Rabbit Unpurified Monoclonal Antibody
Description:
Anti-NGF Receptor Antibody, clone EP1039Y, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes NGF Receptor.
Molecular Weight:
~75 kDa
Immunogen:
Synthetic peptide corresponding to residues of human NGF Receptor.
Clone:
EP1039Y
Isotype:
IgG
Background Information:
Nerve Growth Factor Receptor, also termed NGFR p75, p75NTR, or CD271 is the low affinity NGFR (LNGFR) which binds NGF and other neurotrophins including BDNF, NT3, NT4/5 with similar, low affinity. NGFR p75 is a 75 kDa transmembrane glycoprotein (399 a.a.) consisting of an extracellular domain (222 a.a.) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22 a.a.), and a cytoplasmic domain (155 a.a.). NGFR p75 is mainly expressed in Schwann cells and neurons and in a variety of non neuronal cells. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, mouse, and rat.
Application Notes:
Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected NGF Receptor in human brain glioma tissue.
Immunocytochemistry Analysis: A 1:50 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
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Control:
PC12 cell lysate
Quality Assurance:
Evaluated by Western Blot on PC12 cell lysates.
Western Blot Analysis: 1:2,000-50,000 dilution of this antibody was used to detect NGF Receptor in PC12 cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P08138
Entrez Gene Number:
NP_002498.1
Gene Symbol:
Beta-NGF
HSAN5
MGC161426
MGC161428
NGFB
NID67
OTTHUMP00000013653
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Alternate Names:
beta-nerve growth factor
nerve growth factor (beta polypeptide)
nerve growth factor, beta polypeptide
nerve growth factor, beta subunit
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry (Paraffin)
Western Blotting
Immunocytochemistry