标签归档：ncam

VP-C358   CD56 (NCAM)   品牌 Vector

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CD56 (NCAM)

货号： VP-C358      产品名称： CD56 (NCAM)   品牌： Vector 规格： 1 ml 询盘

Functional Aspects:
CD56 is also known as neural cell adhesion molecule, and found on neurons, astrocytes, Schwann cells, Natural Killer cells and a subset of activated T lymphocytes. This antibody will help identify CD56 expression on n o rmal and uncharacterized tissues.   

MABN221   Anti-NCAM1, clone 11G7.1   品牌 Millipore

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Anti-NCAM1, clone 11G7.1

货号： MABN221      产品名称： Anti-NCAM1, clone 11G7.1   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-NCAM1, clone 11G7.1
Species Reactivity Key Applications Host Format Antibody Type
M, R  WB, IHC Mouse Purified Monoclonal Antibody
Description:
Anti-NCAM1 Antibody, clone 11G7.1
Promotional Text:
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100% Performance Guaranteed
Specificity:
This antibody recognizes the cytoplasmic domain of NCAM1.
Molecular Weight:
~180 kDa observed. Uniprot describes 3 isoforms produced by alternative splicing at ~94 kDa, ~80 kDa, and ~67 kDa. The calculated molecular weight of this protein is 120 kDa, but can be observed between ~105-200 kDa due to high glycosylation (Patel, K., et al. (1991). Br J Cancer Suppl. 14:20-13).
Epitope:
cytoplasmic domain
Immunogen:
GST-tagged recombinant protein corresponding to the cytoplasmic domain of mouse NCAM1.
Clone:
11G7.1
Isotype:
IgG1κ
Background Information:
The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulin proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural evelopment. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.
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Species Reactivity:
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Mouse and Rat.
Application Notes:
Western Blot Analysis: 0.5 µg/mL from a representative lot detected NCAM1 in 10 µg of mouse brain tissue lysate.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected NCAM1 in rat hippocampus and rat cerebral cortex tissues.
Control:
Rat brain tissue lysate
Quality Assurance:
Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NCAM1 in 10 µg of rat brain tissue lysate.
Purification Method:
Protein G purified
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P13595
Entrez Gene Number:
NP_001074914
Gene Symbol:
Ncam1
Ncam
Alternate Names:
Neural cell adhesion molecule 1
N-CAM-1
NCAM-1
CD56
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Product Name:
Anti-NCAM1, clone 11G7.1
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.< 2-8℃

AB5032   NCAM, RB X CH-50UG   品牌 Millipore

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NCAM, RB X CH-50UG

货号： AB5032      产品名称： NCAM, RB X CH-50UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Neural Cell Adhesion Molecule Antibody
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REFERENCES
High-resolution array CGH profiling identifies Na/K transporting ATPase interacting 2 (NKAIN2) as a predisposing candidate gene in neuroblastoma.
Romania, Paolo, et al. (2013) PLoS ONE, 8: e78481 (2013)
Low prefrontal PSA-NCAM confers risk for alcoholism-related behavior.
Jacqueline M Barker,Mary M Torregrossa,Jane R Taylor (2012) Nature neuroscience.15
Overexpression of inducible 70-kDa heat shock protein in mouse improves structural and functional recovery of skeletal muscles from atrophy.
Elen H Miyabara,Tabata L Nascimento,Débora C Rodrigues,Anselmo S Moriscot,Wilmer F Davila,Younss Aitmou,Pieter P Detombe,Ruben Mestril (2012) Pflügers Archiv : European journal of physiology.463
Kokumi substances, enhancers of basic tastes, induce responses in calcium-sensing receptor expressing taste cells.
Yutaka Maruyama,Reiko Yasuda,Motonaka Kuroda,Yuzuru Eto (2012) PloS one.7
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Species Reactivity Key Applications Host Format Antibody Type
Ch, H, M, R  ELISA, IF, IHC, FUNC, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Neural Cell Adhesion Molecule Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
Neural Cell Adhesion Molecule (NCAM). MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.
Molecular Weight:
MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.
Immunogen:
Highly purified chicken NCAM
Background Information:
The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulins proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural development. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.
View All »
Species Reactivity:
Chicken
Human
Mouse
Rat
Species Reactivity Note:
Mouse. Expected to cross-react with Chicken, Human, and rat.
Application Notes:
Immunohistochemistry:
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 μg/mL and mouse NCAM using a concentration of 2 μg/mL.
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Function blocking:
A previous lot of this antibody was used in Function blocking.
ELISA:
0.3 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
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Control:
Neuraminidase-treated mouse brain membrane.
Quality Assurance:
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 μg of mouse brain lysate.
P -20℃