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04-903   Anti-GSK3a/b 100ul   品牌 Millipore

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Anti-GSK3a/b 100ul

货号： 04-903      产品名称： Anti-GSK3a/b 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-GSK3α/β
Recommended Replacement for: 05-903
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PRODUCT FAMILY INFORMATION
GSK-3 Antibodies, Proteins, Inhibitors
Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways.
Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. EMD Millipore’s GSK-3 antibodies, proteins and inhibitors are based on the expertise of Upstate, Chemicon, and Calbiochem. Our GSK-3 reagents have been well validated and published. See the ordering tab for a comprehensive list of our GSK-3 products and direct links to their technical pages for data, references and related products.
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-GSK3α/β Antibody, rabbit monoclonal
Replaces:
05-903
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
GSK3 alpha and beta.
Molecular Weight:
~46 kDa (beta) and 51 kDa (alpha)
Immunogen:
N-terminal 6His-tagged recombinant full length human GSK3β.
Isotype:
IgG
Background Information:
Glycogen Synthase Kinase 3 differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer’s disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human and mouse.
Predicted to cross-react with rat based on sequence homology.
Application Notes:
Immunoprecipitation: 2-8 μL of a previous lot of antibody immunoprecipitated GSK3 alpha and beta from 100 μg of HEK293 RIPA lysate.
NOTE: This antibody also successfully immunoprecipitated GSK3 alpha and beta using the Catch and Release® v2.0 Reversible Immunoprecipitation System (Catalog # 17-500).
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Control:
HEK293 cell lysate
Quality Assurance:
Evaluated by western blot on lysates from HEK293 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GSK3 alpha and GSK3 beta in RIPA lysates from A431, HEK293, Jurkat, HeLa NE or 3T3/A31 cells.
Presentation:
Cultured supernantant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P49840
Entrez Gene Number:
NP_063937.2, NP_002084.2
Gene Symbol:
DKFZp686D0638
EC 2.7.11.26
Alternate Names:
GSK-3 alpha
glycogen synthase kinase 3 alpha
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only a -20°C

05-903   Anti-GSK3a/b   品牌 Millipore

发表于由

Anti-GSK3a/b

货号： 05-903      产品名称： Anti-GSK3a/b   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-GSK3α/β Antibody, rabbit monoclonal
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REFERENCES
The neuron-specific isoform of glycogen synthase kinase-3beta is required for axon growth.
Castaño, Zafira, et al. (2010) J. Neurochem., 113: 117-30 (2010)
The sonic hedgehog signaling pathway is reactivated in human renal cell carcinoma and plays orchestral role in tumor growth.
Dormoy, Valerian, et al. (2009) Molecular cancer, 8: 123 (2009)
GSK-3 inhibitors: discoveries and developments.
Alonso, M and Martinez, A (2004) Curr. Med. Chem., 11: 755-63 (2004)
The interaction of glycogen synthase kinase-3 (GSK-3) with the cell cycle.
Ryves, W Jonathan and Harwood, Adrian J (2003) Prog Cell Cycle Res, 5: 489-95 (2003)
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Species Reactivity Key Applications Host Format Antibody Type
H, M  IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-GSK3α/β Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
GSK3 alpha and beta
Molecular Weight:
Mr 46kDa (beta) and 51kDa (alpha)
Immunogen:
N-terminal 6His-tagged recombinant full length human GSK3β
Isotype:
IgG
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Predicted to cross-react with rat based on sequence homology
Quality Assurance:
routinely evaluated by immunoblot on lysates from HEK293 cells
Purification Method:
Protein A purfied
Storage Conditions:
stable 2 years at -20°C from date of shipment
UniProt Number:
P49840
Entrez Gene Number:
NM_019884/NM_002093
Gene Symbol:
GSK3A
DKFZp686D0638
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila ‘shaggy’ (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM]
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UniProt Summary:
FUNCTION: SwissProt: P49840 # Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN (By similarity).
SIZE: 483 amino acids; 50981 Da
SUBUNIT: Monomer (By similarity).
SIMILARITY: SwissProt: P49840 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain.
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Brand Family:
Upstate
Product Name:
Anti-GSK3α/β Antibody, rabbit monoclonal
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Purified
Host:
Rabbit
Applications:
Anti-GSK3α/β Antibody is an antibody against GSK3α/β for use in IP & WB.

-20°C

07-393   ANTI-PHOSPHO-GSK3A (SER21) 100UL   品牌 Millipore

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ANTI-PHOSPHO-GSK3A (SER21) 100UL

货号： 07-393      产品名称： ANTI-PHOSPHO-GSK3A (SER21) 100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-GSK3α (Ser21) Antibody
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REFERENCES
The alpha-isoform of glycogen synthase kinase-3 from rabbit skeletal muscle is inactivated by p70 S6 kinase or MAP kinase-activated protein kinase-1 in vitro.
Sutherland, C and Cohen, P (1994) FEBS Lett., 338: 37-42 (1994)
Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling.
Sutherland, C, et al. (1993) Biochem. J., 296 ( Pt 1): 15-9 (1993)
Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B.
Cross, D A, et al. (1995) Nature, 378: 785-9 (1995)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-phospho-GSK3α (Ser21) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
phospho-GSK3α (Ser21)
Molecular Weight:
51kDa
Immunogen:
Peptide corresponding to amino acids 16-26 of human phospho-GSK3alpha (Ser21) with an N-terminal cysteine.
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
Routinely evaluated by immunoblot in RIPA lysates from HEK293 cells stimulated with 200ng/ml of IGF1 for 5 minutes
Purification Method:
Protein A purfied
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
P49840
Entrez Gene Number:
NM_019884
Gene Symbol:
GSK3A
DKFZp686D0638
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila ‘shaggy’ (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM]
View All »
UniProt Summary:
FUNCTION: SwissProt: P49840 # Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN (By similarity).
SIZE: 483 amino acids; 50981 Da
SUBUNIT: Monomer (By similarity).
SIMILARITY: SwissProt: P49840 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain.
View All »
Brand Family:
Upstate
Product Name:
Anti-phospho-GSK3α (Ser21) Antibody
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Purified
Host:
Rabbit
Applications:
Anti-phospho-GSK3α (Ser21) Antibody detects level of phospho-GSK3α (Ser21) & has been published & validated for use in WB.

-20℃