标签归档：er

E1390   pBIND-ERα Vector   品牌 Promega

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pBIND-ERα Vector

货号： E1390      产品名称： pBIND-ERα Vector   品牌： Promega 规格： 20μg 二周到货 生化试验

说明  
 核受体检测可以通过传统的方法即利用上游带有核受体反应元件的最小启动子载体来完成。您也可以选择病毒元件像小鼠乳腺肿瘤病毒长末段重复启动子来检测雌激素或糖皮质激素的响应( 如pGL4.36)。很多情况下，利用这些方法进行的研究需要选择含有合适的内源核受体的细胞系，就是说每一种核受体的研究可能需要选择不同的细胞系。利用酵母双杂交原理的方法也适用于核受体的研究。核受体配基结合域与GAL4 DNA 结合域相融合，通过上游含有GAL4 上游激活序列的重复片段的最小启动子的萤火虫萤光素酶载体转染至细胞中。配基结合区域会与配基结合形成同源或异源的二聚体，以及与激活因子或抑制因子相互作用。这种单杂交系统可帮助您在很多细胞系中研究您所感兴趣的核受体。
特点    • 强劲：基于GAL4 的系统从内源性受体中去除了背景信号。
• 更灵敏：9X Gal4 经过优化，反应性增强，信噪比更高。
• 适应性强：海肾/ 新霉素的组合标记的引入，使得只需一个载体，既可用双萤光素酶检测试剂盒进行归一化，又可用选择性标记筛选生成的稳定细胞系。
• 稳定如一：仅需使用一个实验体系，就可以将所有的核受体进行比较或者研究其特征。
• 更快得到结果：去稳定化的luc2P 的萤光素酶报告基因经过优化，具有更高的灵敏度和更短的诱导时间。

 

BML-UW0175-0100   (E93R)SUMO-1 (human) (recombinant) (GST)   品牌 Biomol

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(E93R)SUMO-1 (human) (recombinant) (GST)

货号： BML-UW0175-0100      产品名称： (E93R)SUMO-1 (human) (recombinant) (GST)   品牌： Biomol 规格： 100 ug 3周到货 生化实验

[E93R]SUMO-1 (human), (recombinant) (GST-tag)
C-Terminal mutant of mature SUMO-1 protein for use in proteomic investigations. The use of this mutant SUMO-1 for the modification of target proteins, with recovery of resulting SUMO conjugates using the GST-tag, followed by tryptic digestion gives rise to peptides with a SUMO specific side chain signature not readily obtainable using wild-type SUMO modification. This modification allows ready differentiation by mass spectrometric analysis of SUMO modifications from those derived from ubiquitin and other Ubls.
Product Specification
UniProt ID: P63165
Application: Identification of SUMO modified proteins by proteomic methods. Identification of SUMO modification sites by proteomic methods.
Long Term Storage: -80°C

-80°C

01-189   IGF-I (E3R) 25UG   品牌 Millipore

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IGF-I (E3R) 25UG

货号： 01-189      产品名称： IGF-I (E3R) 25UG   品牌： Millipore 规格： EA 3周到货 生化实验

IGF-I, somatomedins, insulin-like growth factors (resistant to IGFBPs)
Description:
IGF-I (resistant to IGFBPs)
Trade Name:
Upstate (Millipore)
Qty/Pk:
25 µg
Key Applications:
Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed ‘sulfation factor,’ which later became known as ‘somatomedin’ (Daughaday et al., 1972 [PubMed 4550398]). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986 [PubMed 3455760]; Rosenfeld, 2003 [PubMed 14657423]).[supplied by OMIM]
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UniProt Summary:
FUNCTION: SwissProt: P05019 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.| P01343 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.
SIZE: 195 amino acids; 21841 Da
SUBCELLULAR LOCATION: Secreted.
DOMAIN: SwissProt: P05019
DISEASE: SwissProt: P05019 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.| P01343 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
SIMILARITY: SwissProt: P05019 ## Belongs to the insulin family.| P01343 ## Belongs to the insulin family.
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Species:
Human
Brand Family:
Upstate
Source:
Recombinant human IGF-I (ER3) expressed in E. coli
Presentation:
Lypholized from acetic acid
Gene Symbol:
IGF1
IBP1
IGFI
IGF-IA
MGF
IGF-IB
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Product Name:
IGF-I (resistant to IGFBPs)
Quality Assurance:
Routinely evaluated in a biological activity assay.
Physical Form:
Lyophilized
UniProt Number:
P05019
P01343
Entrez Gene Number:
NM_000618.2
Purification Method:
Sterilized through a 0.2 micron membrane filter

04-227   Anti-ER alpha   品牌 Millipore

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Anti-ER alpha

货号： 04-227      产品名称： Anti-ER alpha   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Estrogen Receptor α, clone E115 Antibody
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, ICC, IH(P) Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-Estrogen Receptor α Antibody, clone E115, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes Estrogen Receptor α.
Molecular Weight:
67 kDa
Immunogen:
Synthetic peptide directed against human Estrogen Receptor α.
Clone:
E115
Isotype:
IgG
Background Information:
Estrogen Receptor α (ER alpha) is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Proven to react with human, mouse and rat.
Application Notes:
Immunohistochemistry:
Immunocytochemistry:
1:100 dilution of a previous lot was used on immunocytochemistry.
Control:
MCF-7 cell lysate.
Quality Assurance:
Routinely evaluated by western blot on MCF-7 cell lysate.
Western Blot Analysis:
A 1:250-1:500 dilution of this lot was used to detect Estrogen Receptor  in MCF-7 cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P03372
Entrez Gene Number:
NP_000116.2
Gene Symbol:
ER
ER-alpha
ESR
ESRA
Era
NR3A1
DKFZp686N23123
OTTHUMP00000017718
OTTHUMP00000017719
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Alternate Names:
Estradiol receptor
Nuclear receptor subfamily 3 group A member 1
estrogen receptor 1
estrogen receptor alpha
oestrogen receptor
steroid hormone receptor
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffi -20°C