ANTI-CUG-BP1, PC 12-309

货号： 05-621      产品名称： ANTI-CUG-BP1, PC 12-309   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-CUGBP1 Antibody, clone 3B1
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REFERENCES
HTS-Compatible Patient-Derived Cell-Based Assay to Identify Small Molecule Modulators of Aberrant Splicing in Myotonic Dystrophy Type 1.
Debra A O’Leary,Leonardo Vargas,Orzala Sharif,Michael E Garcia,Yury J Sigal,Siu-Kei Chow,Christian Schmedt,Jeremy S Caldwell,Achim Brinker,Ingo H Engels (2010) Current chemical genomics.4
Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.
Timchenko, L T, et al. (1996) Nucleic Acids Res., 24: 4407-14 (1996)
Species Reactivity Key Applications Host Format Antibody Type
B, H, M, Po, R, Rb  EMSA, IP, WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-CUGBP1 Antibody, clone 3B1
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Trade Name:
Upstate (Millipore)
Specificity:
CUG-BP1
Molecular Weight:
50kDa
Immunogen:
Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50
Isotype:
IgG
Background Information:
Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3′-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG)8 oligonucleotides in vitro. CUG-BP1 is the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3′-UTR.
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Species Reactivity:
Bovine
Human
Mouse
Pig
Rat
Rabbit
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Control:
HeLa lysates
Quality Assurance:
routinely evaluated by immunoblot on HeLa nuclear extract
Purification Method:
Protein G chromatography
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Q92879
Entrez Gene Number:
NM_006560 and others
Gene Symbol:
CUGBP1
BRUNOL2
CELF-1
CUGBP
NAB50
CELF1
CUG-BP
hNab50
CUG-BP1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Electrophoretic Mobility Shift Assay
Immunoprecipitation
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase -20°C