BMX, Active

货号： 7732-5      产品名称： BMX, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active BMX
Interact with PAK1, PTK2, PTPN21 and RUFY1
Product Overview
Alternate Names / Synonyms: Active BMX, BMX, human BMX, active kinase, active kinases, recombinant BMX, recombinant BMX, kinase activity assay, protein kinase
Gene Symbol: BMX
Accession #: P51813
Gene ID: 660
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: 110.0 kDa
Purity by SDS-PAGE: ≥80%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 29 nmol phosphate incorporated into Poly Glu-Tyr substrate per minute per mg protein at 30°C using a final concentration of 50 µM ATP (0.83 µCi/assay).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: The BMX gene encodes a novel non receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells (1). Bmx cDNA comprises a long open reading frame of 675 amino acids, containing one SH3, one SH2 and one tyrosine kinase domain, which are about 70% identical with Btk, Itk and Tec and somewhat less with Txk tyrosine kinase sequences. The amino terminal sequences of these four tyrosine kinases are about 40% identical and each contains a so-called pleckstrin homology (PH) domain. The 2.7 kb Bmx mRNA is expressed in endothelial cells and several human tissues by Northern blotting and an 80 kD Bmx polypeptide was detected in human endothelial cells. The BMX gene is located in chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked aγglobulinemia. Bmx, is found to induce activation of the Stat signaling pathway (3). Bmx induced the tyrosine phosphorylation and DNA binding activity of all the Stat factors tested, including Stat1, Stat3, and Stat5, both in mammalian and insect cells. Bmx also induced transcriptional activation of Stat1- and Stat5-dependent reporter genes.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C