标签归档：apf

AP512F   Sheep anti-Human IgM (Fc) FITC conjugate   品牌 Millipore

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Sheep anti-Human IgM (Fc) FITC conjugate

货号： AP512F      产品名称： Sheep anti-Human IgM (Fc) FITC conjugate   品牌： Millipore 规格： EA 三周到货 生化实验

Sheep anti-Human IgM, (Fc), FITC conjugate
Species Reactivity Key Applications Host Format Antibody Type
H  FC Sheep FITC Polyclonal Antibody
Description:
Sheep Anti-Human IgM, (Fc), FITC conjugate Antibody
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Specificity:
This antibody reacts with human IgM, (Fc) as demonstrated by Flow Cytometry.
Molecular Weight:
50 kDa calculated
Epitope:
IgM, (Fc)
Immunogen:
Human anti-Human IgM (myeloma) Fc5u fragment
Background Information:
Immunoglobin M (IgM) antibody is prominent in early immune responses to most antigens and is the predominant response in certain antibody responses such as "natural" blood group antibodies. IgM is the major immunoglobin expressed on surface B lymphocytes. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt’s lymphoma bear surface IgM, whereas plasmocytes from Waldenström’s disease bear intracytoplasmic IgM.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. No reactivity with IgG or IgA. May react with IgM of other species with low specificity.
Control:
Human hybridoma expressing EBV
Quality Assurance:
Evaluated by Flow Cytometry in human hybridoma expressing EBV.
Flow Cytometry Analysis: 1:4,000 dilution of this antibody detected Sheep Anti-Human IgM, (Fc) in human hybridoma expressing EBV.
Purification Method:
Depletion
Presentation:
Purified sheep polyclonal in PBS containing 0.05% sodium azide and 15 mg/mL BSA. Depleted against rabbit IgM and human IgG.
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light in undiluted aliquotes for up to 6 months from date of receipt.
UniProt Number:
P01871
Gene Symbol:
IGHM
Alternate Names:
Ig mu chain C region
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
UniProt Summary:
FUNCTION: IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as an homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts.
SUBUNIT STRUCTURE: Immunoglobulin (Ig) molecules consist of two chains, one heavy chain (which can be alpha, delta, epsilon, gamma or mu) and one light chain (which can be kappa or lambda) each consisting of a variable and a constant region. An IgM molecule contains thus a mu heavy chain combined with either a kappa or a lambda light chains. It is found almost exclusively as an homopentamer in the serum. Membrane-bound IgM molecules are non-covalently associated with heterodimer of CD79A and CD79B.
SUBCELLULAR LOCATION: Isoform 1: Secreted. Note: During di 2-8℃

10157-500   APF   品牌 Cayman

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APF

货号： 10157-500      产品名称： APF   品牌： Cayman 规格： 500 碌g 3周

APF Cayman Chemical Item Number 10157 Description The biology of highly reactive oxygen radical species is of great interest in many biomedical research disciplines, including, neurodegeneration and aging, cancer, and infectious diseases.1↗ There are a number of fluorescent reagents that can be used to detect free radicals, such as 2,7-dichlorodihydrofluorescein (DCDHF), but they have significant limitations due to their facile oxidation by light and numerous non-radical oxidants such as hydrogen peroxide (H2O2).2↗ APF is an aromatic amino-fluorescein derivative that has little intrinsic fluorescence. It undergoes oxidation only by the hydroxyl radical, hypochlorite ion, and certain peroxidase intermediates, but is inert to NO, H2O2, superoxide, and other oxidants. Upon oxidation, APF is converted to the highly fluorescent molecule fluorescein, allowing the simple direct detection of highly reactive biological radicals.3↗ Formal Name 2-​[6-​(4-​aminophenoxy)-​3-​oxo-​3H-​xanthen-​9-​yl]-​benzoic acid CAS Number 359010-70-1 Molecular Formula C26H17NO5 Formula Weight 423.4 Formulation A solution in methyl acetate Purity >98% λmax 223, 278 nm Stability 1 year Storage -20°C Shipping Room temperature in continental US; may vary elsewhere SMILES Nc1ccc(cc1)​Oc1ccc2c(c1)​Oc1cc(O)​ccc1C12OC(=O)​c2ccccc12 InCHI Code ​1S/C26H17NO5/c27-15-5-8-17(9-6-15)​30-18-10-12-22-24(14-18)​31-23-13-16(28)​7-11-21(23)​26(22)​20-4-2-1-3-19(20)​25(29)​32-26/h1-14,28H,27H2 InCHI Key SKRNNOWWEXPZBB-UHFFFAOYSA-N -20°C

16050   ROS Brite APF   品牌 AAT

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ROS Brite APF

货号： 16050      产品名称： ROS Brite APF   品牌： AAT 规格： 1 mg 二周到货 生化试验

ROS Brite� APF
Cat # 16050
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 492
Em (nm) 515
MW 423.42
Solvent DMSO
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite� APF is a fluorogenic probe to measure hydroxyly radical in cells using conventional fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The cell-permeant ROS Brite� APF reagent is nonfluorescent and produces bright green fluorescence upon reaction with hydroxyl radical. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. In the presence of peroxidase, APF also reacts with hydrogen peroxide. APF has good selectivity to hydroxyl radical compared to other ROS. APF and HPF show relatively high resistance to light-induced oxidation. APF and HPF are nonfluorescent until they react with the hydroxyl radical or peroxynitrite anion. APF will also react with the hypochlorite anion.
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Reactive Oxygen Species     Cell Signaling 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)