标签归档：amylase

K711-100   Amylase Activity Assay Kit   品牌 Biovision

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Amylase Activity Assay Kit

货号： K711-100      产品名称： Amylase Activity Assay Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

Amylase Activity Colorimetric Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (405 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples etc.
• Species reactivity- Mammalian
• Application- The assay can detect α-amylase content as low as 0.2 mU.
Features & Benefits:
• Simple procedure; takes ~ less than 40 minutes
• Fast and convenient
• Sensitive assays for measuring Amylase activity in various biological samples.
Kit components:
• Amylase Assay Buffer
• Amylase Substrate Mix
• Amylase Positive Control
• Nitrophenol standard (2 mM)
Description:
Amylases are enzymes that break starch down to sugar molecules. α-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker’s yeast for instance). α-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. Increased enzyme levels in humans are associated with salivary trauma; mumps due to inflammation of the salivary glands, pancreatitis and renal failure. A simple, direct and automation-ready procedure for measuring α-amylase activity is, therefore, very desirable. BioVision’s α-amylase Assay uses ethylidene-pNP-G7 as the substrate. Once the substrate has been specifically cleaved by α-amylase, the smaller fragments produced can be acted upon by α-glucosidase, which causes the ultimate release of the chromophore that can then be measured at 405 nm. The assay can detect α-amylase content as low as 0.2 mU.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

3014-100   Alpha-Amylase Antibody   品牌 Biovision

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Alpha-Amylase Antibody

货号： 3014-100      产品名称： Alpha-Amylase Antibody   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Alpha-Amylase Antibody
Product Overview
Antibody Target: Amylase
Alternative Name: Alpha-amylase 1, 1,4-alpha-D-glucan glucanohydrolase 1, Salivary alpha-amylase
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide surrounding amino acid 492 of human α-Amylase
Accession#: P04745
Gene ID: 276
Appearance: Colorless liquid
Concentration: 0.5 mg/ml.
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti- α-Amylase polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Application: Western blot
Application & Usage: Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: α-Amylase is an enzyme that is normally produced in the saliva glands and pancreas in humans and in other mammals. α-Amylase catalyzes the cleavage of 1, 4-α-D-glucosidic bonds of large sugar molecules into oligosaccharides and polysaccharides
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

3102-100   Rat Pancreatic Amylase Antibody (Clone RPA-B5)   品牌 Biovision

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Rat Pancreatic Amylase Antibody (Clone RPA-B5)

货号： 3102-100      产品名称： Rat Pancreatic Amylase Antibody (Clone RPA-B5)   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Rat Pancreatic Amylase Antibody (Clone RPA-B5)
Product Overview
Antibody Target: Pancreatic Amylase
Alternative Name: Amylase, pancreatic amylase, rat pancreatic amylase
Host: Mouse
Antibody Type: Monoclonal (Clone RPA-B5)
Isotype: Mouse IgG1
Immunogen: Recombinant protein expressed in E. coli
Accession#: P00689
Gene ID: 497039
Appearance: Colorless liquid
Concentration: 1.0 mg/ml
Formulation: 100 µg (1.0 mg/ml) purified mouse monoclonal antibody supplied in PBS with 0.05% (W/V) sodium azide.
Purification: Affinity purified
Species Reactivity: rat
Application: Indirect ELISA, Western blot
Application & Usage: The antibody can be used for indirect ELISA and WB.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Pancreatic amylase is a digestive enzyme secreted by pancreas into the intestine. It consists of a single polypeptide chain with a molecular weight of 54 kDa. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.

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3925-100   Alpha-Amylase Antibody   品牌 Biovision

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Alpha-Amylase Antibody

货号： 3925-100      产品名称： Alpha-Amylase Antibody   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Alpha-Amylase Antibody
Product Image
Alpha-Amylase Antibody
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Product Overview
Antibody Target: Alpha-Amylase
Alternative Name:
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide surrounding amino acid 273 of rat α-Amylase
Accession#: NP_001010970
Gene ID: 24203
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat, pig, chickenen
Application: Western blot
Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~58 kDa α-Amylase in samples from human, mouse and rat origins. Reactivity to other species has not been tested.
Storage Temp.: -20 °C
Shipping:  gel pack
Background Descriptions: α-Amylase is an enzyme that is normally produced in the saliva glands and pancreas in humans and in other mammals. α-Amylase catalyzes the cleavage of 1, 4-α-D-glucosidic bonds of large sugar molecules into oligosaccharides and polysaccharides.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.

-20 °C

3014BP-50   Alpha- Amylase Blocking Peptide   品牌 Biovision

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Alpha- Amylase Blocking Peptide

货号： 3014BP-50      产品名称： Alpha- Amylase Blocking Peptide   品牌： Biovision 规格： 50 μg 3周到货 生化实验

Alpha- Amylase Blocking Peptide
Product Overview
Antibody Target: Alpha- Amylase
Accession #: P04745
Gene ID: 276
Appearance: Colorless liquid
Concentration: 0.5mg/ml
Formulation: 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal
Storage Temp.: -20°C
Shipping: Gel Pack
Application & Usage: The peptide is used for blocking the antibody activity of α-Amylase (Cat.# 3014-100). It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C
Description: 50 µg blocking peptide for α-Amylase polyclonal antibody(Cat.# 3014-100)
Handling: The peptide solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

3925BP-50   Alpha-Amylase Blocking Peptide   品牌 Biovision

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Alpha-Amylase Blocking Peptide

货号： 3925BP-50      产品名称： Alpha-Amylase Blocking Peptide   品牌： Biovision 规格： 50 μg 3周到货 生化实验

Alpha-Amylase Blocking Peptide
Product Name: Alpha-Amylase Blocking Peptide
Antibody Target: Synaptotagmin
Accession #: P21707
Gene ID: 25716
Appearance: Colorless liquid
Concentration: 0.5mg/ml
Formulation: 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Storage Temp.: -20°C
Shipping: Gel Pack
Application & Usage: The peptide is used for blocking the antibody activity of synaptotagmin (Cat. # 3177-100). It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Description: 50 ug blocking peptide for synaptotagmin polyclonal Antibody (Cat. # 3177-100).
Handling: The peptide solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.

-20°C