标签归档：tag

4895A-100   C5a (His-Tag), murine recombinant   品牌 Biovision

发表于由

C5a (His-Tag), murine recombinant

货号： 4895A-100      产品名称： C5a (His-Tag), murine recombinant   品牌： Biovision 规格： 100 μg 3周到货 生化实验

C5a (His-Tag), murine recombinant
An anaphylatoxin
Product Overview
Alternate Names / Synonyms: Recombinant murine C5a (His-Tag), murine C5a, m-C5a, rm-C5a, recombinant murine C5a, murine C5a (His-tag), complement C5a, Complement Component C5a, Complement component 5a, complement fragment C5a, Complement Fragments C5a, complement-derived C5a, Complement C5a Protein
Gene Symbol: C5a
Accession #: NP_034536
Gene ID: 15139
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and lyophilized with no additives
Molecular Weight: 9 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: <0.1 ng/μg
Biological Activity: ED₅₀ 1-3 nM as determined by enzyme release assay of myeloperoxidase
Reconstitution Instructions: Reconstitute to a concentration of 1.0 mg/ml in ice-cold water containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C.
Storage Temp.: -20°C
Shipping: Blue ice
Background Information: Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the α-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. The 9 kDa mouse C5a protein expressed in E. coli contains a N-terminal His-Tag sequence.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-20°C

4895A-1000   C5a (His-Tag), murine recombinant   品牌 Biovision

发表于由

C5a (His-Tag), murine recombinant

货号： 4895A-1000      产品名称： C5a (His-Tag), murine recombinant   品牌： Biovision 规格： 1 mg 3周到货 生化实验

C5a (His-Tag), murine recombinant
An anaphylatoxin
Product Overview
Alternate Names / Synonyms: Recombinant murine C5a (His-Tag), murine C5a, m-C5a, rm-C5a, recombinant murine C5a, murine C5a (His-tag), complement C5a, Complement Component C5a, Complement component 5a, complement fragment C5a, Complement Fragments C5a, complement-derived C5a, Complement C5a Protein
Gene Symbol: C5a
Accession #: NP_034536
Gene ID: 15139
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and lyophilized with no additives
Molecular Weight: 9 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: <0.1 ng/μg
Biological Activity: ED₅₀ 1-3 nM as determined by enzyme release assay of myeloperoxidase
Reconstitution Instructions: Reconstitute to a concentration of 1.0 mg/ml in ice-cold water containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C.
Storage Temp.: -20°C
Shipping: Blue ice
Background Information: Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the α-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. The 9 kDa mouse C5a protein expressed in E. coli contains a N-terminal His-Tag sequence.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-20°C

4895A-20   C5a (His-Tag), murine recombinant   品牌 Biovision

发表于由

C5a (His-Tag), murine recombinant

货号： 4895A-20      产品名称： C5a (His-Tag), murine recombinant   品牌： Biovision 规格： 20 μg 3周到货 生化实验

C5a (His-Tag), murine recombinant
An anaphylatoxin
Product Overview
Alternate Names / Synonyms: Recombinant murine C5a (His-Tag), murine C5a, m-C5a, rm-C5a, recombinant murine C5a, murine C5a (His-tag), complement C5a, Complement Component C5a, Complement component 5a, complement fragment C5a, Complement Fragments C5a, complement-derived C5a, Complement C5a Protein
Gene Symbol: C5a
Accession #: NP_034536
Gene ID: 15139
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and lyophilized with no additives
Molecular Weight: 9 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: <0.1 ng/μg
Biological Activity: ED₅₀ 1-3 nM as determined by enzyme release assay of myeloperoxidase
Reconstitution Instructions: Reconstitute to a concentration of 1.0 mg/ml in ice-cold water containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C.
Storage Temp.: -20°C
Shipping: Blue ice
Background Information: Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the α-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. The 9 kDa mouse C5a protein expressed in E. coli contains a N-terminal His-Tag sequence.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-20°C

PF124-20UG   ADAM10, HIS-TAG, MOUSE, RECOMBINANT   品牌 Merck

发表于由

ADAM10, HIS-TAG, MOUSE, RECOMBINANT

货号： PF124-20UG      产品名称： ADAM10, HIS-TAG, MOUSE, RECOMBINANT   品牌： Merck 规格： *** 3周到货 生化实验

ADAM10, His•Tag®, Mouse, Recombinant, S. frugiperda
Kuzbanian Metalloproteinase
Recombinant, mouse ADAM10 (mature, active mouse ADAM10 ectodomain; amino acids 19-673) fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. An integral membrane proteinase involved in notch signaling. Also considered as an α-secretase candidate.
产品信息
Form  Lyophilized
Formulation  Lyophilized from a filtered solution of 100 mM NaCl, 25 mM MES, 10 µM ZnCl2, 0.02% BRIJ®-35 Detergent, pH 5.5.
Molar mass  60000
Applications 
Immunoblotting (Western Blotting)
Substrate Cleavage Assay
Avoid freeze/thaw  是的
Specific activity  ≥5 pmol/min/µg
Activity  ≥5 pmol/min/µg as measured by the cleavage of 10 µM of the fluorescent substrate MCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2 (Cat. No. 616402) by 100 ng of the enzyme in 100 µl of reaction buffer at 37°C.
Purity  ≥90% by SDS-PAGE
Contaminants  Endotoxin: ≤1 EU/µg ADAM10
Solubility  25 mM Tris-HCl buffer, pH 7.5
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Standard Handling

-20°C

PF125-50UG   BACE-1, HIS-TAG, HUMAN, RECOMBINANT   品牌 Merck

发表于由

BACE-1, HIS-TAG, HUMAN, RECOMBINANT

货号： PF125-50UG      产品名称： BACE-1, HIS-TAG, HUMAN, RECOMBINANT   品牌： Merck 规格： *** 3周到货 生化实验

BACE1, His•Tag®, Human, Recombinant, NSO Cells
Recombinant, human BACE-1 (amino acids 1-460) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. Supplied as a mixture of the ~68-70 kDa proenzyme and the ~65-67 kDa mature enzyme. BACE-1 is an aspartic protease that has been shown to cleave the amyloid precursor protein β site in the brain to generate the amyloid β peptide. MW: ~65-70 kDa under reducing conditions.
产品信息
Form  Lyophilized
Formulation  Lyophilized from sterile-filtered PBS.
Preservative  None
Specific activity  ≥1 pmol/min/µg protein
Activity  ≥1 pmol/min/µg as measured by the cleavage of 10 µM MCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(DNP)-Arg-Arg-NH2 fluorescent substrate using 100 ng BACE-1 in 0.1 M sodium acetate (pH 4.0) at room temperature.
Purity  ≥90% by SDS-PAGE
Contaminants  Endotoxin: ≤1 EU/µg BACE-1
Solubility  H2O
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Standard Handling

-20°C

PF133-10UG   TACE, HIS-TAG, RECOMBINANT PROTEIN   品牌 Merck

发表于由

TACE, HIS-TAG, RECOMBINANT PROTEIN

货号： PF133-10UG      产品名称： TACE, HIS-TAG, RECOMBINANT PROTEIN   品牌： Merck 规格： *** 3周到货 生化实验

TACE, His•Tag®, Human, Recombinant, T. ni.
ADAM17, Human α-Secretase
Recombinant, human TACE (amino acids 1-671) fused to a His•Tag® sequence and expressed in T. ni. insect cells. The major form of the purified protein corresponds to the mature form with an apparent molecular mass of ~70 kDa.
产品信息
Form  Lyophilized
Formulation  Lyophilized from sterile-filtered 25 mM Tris-HCl, pH 8.0.
Preservative  None
Biological activity  ≥100 pmol/min/µg as measured by the cleavage of 10 µM of fluorescent substrate (MCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2; Cat. No. 616402) in 100 µl of reaction buffer at 37°C.
Purity  ≥90% by SDS-PAGE
Solubility  H2O
商店和运输信息
Storage  -20°C
Ship  Ambient Temperature Only
Standard Handling

-20°C

371206-10UG   Dkk-1, His-Tag, Mouse, Recombinant   品牌 Merck

发表于由

Dkk-1, His-Tag, Mouse, Recombinant

货号： 371206-10UG      产品名称： Dkk-1, His-Tag, Mouse, Recombinant   品牌： Merck 规格： *** 3周到货 生化实验

Dkk-1, His•Tag®, Mouse, Recombinant
Recombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
产品信息
Form  Lyophilized
Formulation  Lyophilized from 0.2 µm filtered PBS, BSA.
Molar mass  27600
Biological activity  ND50: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.
Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
Purity  ≥95% by SDS-PAGE
Contaminants  Endotoxin: ≤1.0 EU/µg protein
Solubility  PBS
商店和运输信息
Storage  -20°C
Ship  Ambient Temperature Only
Regulatory Review

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440319-50UG   Lumican, His-Tag, CF, Mouse, Recombinant   品牌 Merck

发表于由

Lumican, His-Tag, CF, Mouse, Recombinant

货号： 440319-50UG      产品名称： Lumican, His-Tag, CF, Mouse, Recombinant   品牌： Merck 规格： *** 3周到货 生化实验

Lumican, His•Tag®, CF, Mouse, Recombinant
Recombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.
产品信息
Form  Lyophilized
Formulation  Lyophilized from 0.2 µm filtered PBS.
Molar mass  37300
Biological activity  Cell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 104 BCE C/D-1b bovine cornea-derived endothelial cells.
Purity  ≥95% by SDS-PAGE
Contaminants  Endotoxin: ≤1.0 EU/µg protein
Solubility  PBS
商店和运输信息
Storage  -20°C
Ship  Ambient Temperature Only
Standard Handling

-20°C

565834-10UG   sFRP-3, His-Tag, Mouse, Recombinant   品牌 Merck

发表于由

sFRP-3, His-Tag, Mouse, Recombinant

货号： 565834-10UG      产品名称： sFRP-3, His-Tag, Mouse, Recombinant   品牌： Merck 规格： *** 3周到货 生化实验

sFRP-3, His•Tag®, Mouse, Recombinant
Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
产品信息
Form  Lyophilized
Formulation  Lyophilized from 0.2 µm filtered PBS, BSA.
Biological activity  Proliferation Assay:
ED50 = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA:
50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
Purity  ≥95% by SDS-PAGE
Contaminants  Endotxin: ≤1 EU/µg
商店和运输信息
Storage  -20°C
Ship  Ambient Temperature Only
Standard Handling

-20°C