ANTI-TACE (807 – 823) RABBIT PAB
货号： PC491-100UG 产品名称： ANTI-TACE (807 – 823) RABBIT PAB 品牌： Merck 规格： *** 三周 生化实验
Anti-TACE (807 – 823) Rabbit pAb
Anti-TNF-α Converting Enzyme, Anti-ADAM17
Recognizes the ~130 kDa TACE protein in HeLa and Jurkat cells.
产品信息
Host Rabbit
Isotype IgG
Immunogen a synthetic peptide (ASFKLQRQNRVDSKETE) corresponding to amino acids 807-823 of human TACE
Form Liquid
Formulation In PBS.
Species reactivity human, mouse, rat
Preservative ≤0.1% sodium azide
Applications
Immunoblotting (Western Blotting)
Avoid freeze/thaw 是的
Positive control HeLa or Jurkat cells
商店和运输信息
Storage -20°C
Ship Dry Ice Only
Standard Handling
-20°C
PF133-10UG TACE, HIS-TAG, RECOMBINANT PROTEIN 品牌 Merck
TACE, HIS-TAG, RECOMBINANT PROTEIN
货号： PF133-10UG 产品名称： TACE, HIS-TAG, RECOMBINANT PROTEIN 品牌： Merck 规格： *** 3周到货 生化实验
TACE, His•Tag®, Human, Recombinant, T. ni.
ADAM17, Human α-Secretase
Recombinant, human TACE (amino acids 1-671) fused to a His•Tag® sequence and expressed in T. ni. insect cells. The major form of the purified protein corresponds to the mature form with an apparent molecular mass of ~70 kDa.
产品信息
Form Lyophilized
Formulation Lyophilized from sterile-filtered 25 mM Tris-HCl, pH 8.0.
Preservative None
Biological activity ≥100 pmol/min/µg as measured by the cleavage of 10 µM of fluorescent substrate (MCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2; Cat. No. 616402) in 100 µl of reaction buffer at 37°C.
Purity ≥90% by SDS-PAGE
Solubility H2O
商店和运输信息
Storage -20°C
Ship Ambient Temperature Only
Standard Handling
-20°C
7607-20 TACE (TNF-α converting enzyme), human recombinant 品牌 Biovision
TACE (TNF-α converting enzyme), human recombinant
货号： 7607-20 产品名称： TACE (TNF-α converting enzyme), human recombinant 品牌： Biovision 规格： 20 μg 3周到货 生化实验
TACE (TNF-α converting enzyme), human recombinant
Involved in the processing of TNF-α at the surface of cells
Product Overview
Product Name: TACE (TNF-α converting enzyme), human recombinant
Alternate Name/Synonyms: Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, CD156b
Gene Symbol: ADAM17
Accession #: P78536
Gene ID: 6868
Source: Insect cell
Appearance: Lyophilized powder
Physical Form Description: The untagged rh-TACE (TNF-alpha converting enzyme) is supplied as a lyophilized powder.
Molecular Weight: 62 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: Activity of TACE was tested using the synthetic peptide, Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa(Dnp)-NH2, as a substrate.
Reconstitution Instructions: With sterile deionized water at 0.1µg protein/µl.
Storage Temp.: -70°C
Shipping: Blue ice
Background Information: TACE (TNF-alpha converting enzyme, ADAM17) is a membrane-bound Metalloprotease from the ADAM (a disintegrin and Metalloprotease) family of proteins. It cleaves 26kDa type II membrane-bound precursor of TNF-a into 17 kDa mature form which is secreted. TACE is also known to process other membrane proteins including TNF receptor, TGF-alpha and amyloid protein precursor. Recombinant active untagged TACE amino acid sequence is from Arg215 to Asn651. The apparent molecular weight is bigger than the calculated MW due to the protein posttranslational modification in eukaryotic system.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.
-70°C
7607-100 TACE (TNF-α converting enzyme), human recombinant 品牌 Biovision
TACE (TNF-α converting enzyme), human recombinant
货号： 7607-100 产品名称： TACE (TNF-α converting enzyme), human recombinant 品牌： Biovision 规格： 100 μg 3周到货 生化实验
TACE (TNF-α converting enzyme), human recombinant
Involved in the processing of TNF-α at the surface of cells
Product Overview
Product Name: TACE (TNF-α converting enzyme), human recombinant
Alternate Name/Synonyms: Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, CD156b
Gene Symbol: ADAM17
Accession #: P78536
Gene ID: 6868
Source: Insect cell
Appearance: Lyophilized powder
Physical Form Description: The untagged rh-TACE (TNF-alpha converting enzyme) is supplied as a lyophilized powder.
Molecular Weight: 62 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: Activity of TACE was tested using the synthetic peptide, Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa(Dnp)-NH2, as a substrate.
Reconstitution Instructions: With sterile deionized water at 0.1µg protein/µl.
Storage Temp.: -70°C
Shipping: Blue ice
Background Information: TACE (TNF-alpha converting enzyme, ADAM17) is a membrane-bound Metalloprotease from the ADAM (a disintegrin and Metalloprotease) family of proteins. It cleaves 26kDa type II membrane-bound precursor of TNF-a into 17 kDa mature form which is secreted. TACE is also known to process other membrane proteins including TNF receptor, TGF-alpha and amyloid protein precursor. Recombinant active untagged TACE amino acid sequence is from Arg215 to Asn651. The apparent molecular weight is bigger than the calculated MW due to the protein posttranslational modification in eukaryotic system.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.
-70°C
7607-1000 TACE (TNF-α converting enzyme), human recombinant 品牌 Biovision
TACE (TNF-α converting enzyme), human recombinant
货号： 7607-1000 产品名称： TACE (TNF-α converting enzyme), human recombinant 品牌： Biovision 规格： 1 mg 3周到货 生化实验
TACE (TNF-α converting enzyme), human recombinant
Involved in the processing of TNF-α at the surface of cells
Product Overview
Product Name: TACE (TNF-α converting enzyme), human recombinant
Alternate Name/Synonyms: Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, CD156b
Gene Symbol: ADAM17
Accession #: P78536
Gene ID: 6868
Source: Insect cell
Appearance: Lyophilized powder
Physical Form Description: The untagged rh-TACE (TNF-alpha converting enzyme) is supplied as a lyophilized powder.
Molecular Weight: 62 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: Activity of TACE was tested using the synthetic peptide, Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa(Dnp)-NH2, as a substrate.
Reconstitution Instructions: With sterile deionized water at 0.1µg protein/µl.
Storage Temp.: -70°C
Shipping: Blue ice
Background Information: TACE (TNF-alpha converting enzyme, ADAM17) is a membrane-bound Metalloprotease from the ADAM (a disintegrin and Metalloprotease) family of proteins. It cleaves 26kDa type II membrane-bound precursor of TNF-a into 17 kDa mature form which is secreted. TACE is also known to process other membrane proteins including TNF receptor, TGF-alpha and amyloid protein precursor. Recombinant active untagged TACE amino acid sequence is from Arg215 to Asn651. The apparent molecular weight is bigger than the calculated MW due to the protein posttranslational modification in eukaryotic system.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.
-70°C
BML-P132-0500 ADAM17 (TACE) Substrate (Fluorescent) 品牌 Biomol
ADAM17 (TACE) Substrate (Fluorescent)
货号： BML-P132-0500 产品名称： ADAM17 (TACE) Substrate (Fluorescent) 品牌： Biomol 规格： 0.5 mg 3周到货 生化实验
Product Specification
Alternative Name:ADAM17 substrate
Sequence:Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2 [Mca=(7-methoxycoumarin-4-yl)acetyl; Dpa=N-3(2,4-dinitrophenyl)-L-a,ß-diaminopropionyl]
Formula:C69H103N23O24
MW:1638.7
CAS:192723-42-5
Purity:96%
Long Term Storage:-20°C
-20°C
BML-P228-0100 ADAM17 (TACE) Substrate II (Fluorogenic) 品牌 Biomol
ADAM17 (TACE) Substrate II (Fluorogenic)
货号： BML-P228-0100 产品名称： ADAM17 (TACE) Substrate II (Fluorogenic) 品牌： Biomol 规格： 100 ug 3周到货 生化实验
Sensitive fluorogenic substrate for TACE (TNFα converting enzyme, ADAM17). Also cleaved by ADAM10 and likely other ADAMs and MMPs. The kcat/Km of TACE for this substrate is 7-fold higher than for the same peptide with the Mca-Dpa fluorophore-quencher pair. FAM fluorescence is quenched by the TAMRA group until cleavage separates them. Ex.: 485nm, Em.: 535nm, although the following Ex/Em can also be used: 490,494/515,520nm. This substrate is useful for inhibitor screening, kinetic analysis, and cellular activity assay.
Product Specification
Alternative Name:ADAM17 substrate II (fluorogenic)
Sequence:5-FAM-Ser-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Lys(5-TAMRA)-NH2
MW:2157.3
Purity:95%
Long Term Storage:-20°C
-20°C
B50067 ANTI-TACE/ADAM17 (CT) pAb 品牌 Agilent
ANTI-TACE/ADAM17 (CT) pAb
货号： B50067 产品名称： ANTI-TACE/ADAM17 (CT) pAb 品牌： Agilent 规格：
PA112517 TACE 品牌 Pierce
TACE
货号： PA112517 产品名称： TACE 品牌： Pierce 规格： 100 μg
K366-100 TACE Inhibitor Screening Assay Kit 品牌 Biovision
TACE Inhibitor Screening Assay Kit
货号： K366-100 产品名称： TACE Inhibitor Screening Assay Kit 品牌： Biovision 规格： 100 assays 3周到货 生化实验
TACE Inhibitor Screening Assay Kit ( Fluorometric)
Product Overview
Assay Kit Name:
TACE Inhibitor Screening Assay Kit
Kit Summary:
• Detection method- Fluorescence (Ex/Em 318/449 nm)
• Species reactivity- Mammalian
• Applications- Suitable for high-throughput screening of TACE inhibitor and can be modified to check the relative TACE activity. Inhibitor control GM6001 is included to compare the efficiency of test inhibitors.
Features & Benefits:
• Simple procedure
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable.
Kit components:
• TACE Assay Buffer
• TACE Substrate
• TACE Enzyme (20 μg)
• Inhibitor Control (0.1 mM GM6001)
Description:
The TACE (tumor necrosis factor-α-converting enzyme), also called ADAM metallopeptidase domain 17 (ADAM17), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. TACE is believed to be involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. This process, which is also known as ‘shedding’, involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-α), and is of known physiological importance. In BioVision’s TACE inhibitor screening Kit, TACE hydrolyzes the specific FRET substrate to release the quenched fluorescent group, which can be detected fluorometrically at Ex/Em = 318/449 nm. In the presence of the potent TACE inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of TACE inhibitors and can be modified to check the relative TACE activity. Inhibitor Control GM6001 is included to compare the efficacy of test inhibitors.
Storage Conditions:
-20°C
Shipping Conditions:
Gel pack
USAGE: For Research Use Only! Not For Use in Humans.
-20°C
AB19027 TACE, RBX-100UG 品牌 Millipore
TACE, RBX-100UG
货号： AB19027 产品名称： TACE, RBX-100UG 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-ADAM 17 Antibody
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REFERENCES
Sequential Proteolytic Processing of an Interferon-Alpha Receptor Subunit by TNF-Alpha Converting Enzyme and Presenilins.
Peter D Pioli,Abu M Z Saleh,Ashraf El Fiky,Kent L Nastiuk,John J Krolewski (2012) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research.32
Expression analysis of ADAM17 during mouse eye development.
Saadettin Sel,Thomas Kalinski,Isabelle Enssen,Maja Kaiser,Norbert Nass,Stefanie Trau,Gregor Wollensak,Lars Bräuer,Kristin Jäger,Friedrich Paulsen (2012) Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft.194
Epidermal growth factor receptor signaling in hepatocellular carcinoma: inflammatory activation and a new intracellular regulatory mechanism.
Carmen Berasain,Alexandra Nicou,Oihane Garcia-Irigoyen,M Ujue Latasa,Raquel Urtasun,Maria Elizalde,Fabiana Salis,Mar Perugorr,Jes Prieto,Juan A Recio,Fernando J Corrales,Mat Avila (2012) Digestive diseases (Basel, Switzerland).30
Analysis of oestrogen regulation of alpha-, beta- and gamma-secretase gene and protein expression in cultured human neuronal and glial cells.
Linda Csöregh Nord,Johanna Sundqvist,Eva Andersson,Gabriel Fried (2010) Neuro-degenerative diseases.7
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R ICC Rabbit Purified Polyclonal Antibody
Description:
Anti-ADAM 17 Antibody
Promotional Text:
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Trade Name:
Chemicon (Millipore)
Specificity:
TACE is a membrane-bound metallo-proteinase-disintegrin. ADAM 17 also processes other cell surface proteins, including TNF receptor, TGF alpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). ADAM 17 mRNA is expressed in a variety of human and murine tissues.
Molecular Weight:
80 to 130 kDa
Immunogen:
Raised against a peptide corresponding to amino acids 807-823 of human TACE (1,2). This sequence differs from those of mouse and rat TACE by one amino acid.(3).
Background Information:
ADAM17, also known as TNF-alpha Convertase, or TACE, was first cloned from human epithelial cells. Tumor-necrosis factor alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26 kDa Type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately (1-3). The ADAM proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane, and a cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metallo-proteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human and mouse. Expected to react with rat based on sequence of homology.
Application Notes:
Western blot:
1:500 – 1:2,00 -20℃