标签归档：sfrp

565834-10UG   sFRP-3, His-Tag, Mouse, Recombinant   品牌 Merck

发表于由

sFRP-3, His-Tag, Mouse, Recombinant

货号： 565834-10UG      产品名称： sFRP-3, His-Tag, Mouse, Recombinant   品牌： Merck 规格： *** 3周到货 生化实验

sFRP-3, His•Tag®, Mouse, Recombinant
Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
产品信息
Form  Lyophilized
Formulation  Lyophilized from 0.2 µm filtered PBS, BSA.
Biological activity  Proliferation Assay:
ED50 = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA:
50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
Purity  ≥95% by SDS-PAGE
Contaminants  Endotxin: ≤1 EU/µg
商店和运输信息
Storage  -20°C
Ship  Ambient Temperature Only
Standard Handling

-20°C

ALX-201-487-C010   sFRP-5 (human), (recombinant)   品牌 Alexis

发表于由

sFRP-5 (human), (recombinant)

货号： ALX-201-487-C010      产品名称： sFRP-5 (human), (recombinant)   品牌： Alexis 规格： 10 ug 三周 生化实验

sFRP-5 (human), (recombinant) 
Soluble frizzled-related proteins (sFRPs) are modulators of Wnt signaling through direct interaction with Wnts. They regulate cell growth and differentiation in specific cell types. sFRP-5 is an anti-inflammatory adipokine that modulates metabolic dysfunction in obesity. It is secreted by adipocytes and controls the microenvironment of white adipose tissue under conditions of metabolic stress. After secretion sFRP-5 sequesters Wnt5a, preventing noncanonical pathway activation. In the obesity state, adipocytes enlarge and secrete more Wnt5a but less sFRP-5. Macrophages present in the obese adipose tissue respond to Wnt5a excess and produce proinflammatory cytokines that lead to insulin resistance. sFRP-5 was also suggested to be involved in determining the polarity of photoreceptor cells in the retina.
Product Specification
Alternative Name: SARP3, Secreted frizzled-related protein 5
Sequence: Signal peptide and human sFRP-5 (aa 1-317) is fused at the C-terminus to a FLAG-tag.
MW: ~30kDa (SDS-PAGE)
Source: Produced in HEK 293 cells.
UniProt ID: Q5T4F7
Concentration: 0.2mg/ml
Formulation: Liquid. 0.2μm-filtered solution in PBS.
Purity: ≥90% (SDS-PAGE)
Endotoxin Content: <0.1EU/μg purified protein (LAL test; Lonza).
Short Term Storage: +4°C
Long Term Storage: -20°C
Use/Stability: Working aliquots are stable for up to 3 months when stored at -20°C.
Handling: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 

-20°C

09-129   sFRP-4, RBX-100UL   品牌 Millipore

发表于由

sFRP-4, RBX-100UL

货号： 09-129      产品名称： sFRP-4, RBX-100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-SFRP4 Antibody
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REFERENCES
A system-wide investigation of the dynamics of Wnt signaling reveals novel phases of transcriptional regulation.
Taranjit S Gujral,Gavin MacBeath (2010) PloS one.5
Secreted frizzled-related protein 4 is silenced by hypermethylation and induces apoptosis in beta-catenin-deficient human mesothelioma cells
He, Biao, et al (2005) Cancer Res, 65:743-8 (2005)
Secreted Frizzled-related proteins inhibit motility and promote growth of human malignant glioma cells
Roth, W, et al (2000) Oncogene, 19:4210-20 (2000)
Membranous expression of secreted frizzled-related protein 4 predicts for good prognosis in localized prostate cancer and inhibits PC3 cellular proliferation in vitro
Horvath, Lisa G, et al (2004) Clin Cancer Res, 10:615-25 (2004)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Serum Polyclonal Antibody
Description:
Anti-SFRP4 Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes SFRP4
Molecular Weight:
55 kDa
Immunogen:
Recombinant full length human GST-tagged SFRP4 protein.
Isotype:
IgG
Species Reactivity:
Human
Application Notes:
Immunoblot: 1:500-1:5000 dilution is recommended depending on cell type and method of stimulation.
Optimal working dilutions must be determined by end user.
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Serum
UniProt Number:
Q6FHJ7
Entrez Gene Number:
NM_003014.2
Gene Symbol:
SFRP4
sFRP-4
FRPHE
frpHE
MGC26498
FRP-4
FrpHE
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Alternate Names:
secreted frizzled-related protein 4, FRP-4, sFRP-4, FRPHE
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.
UniProt Summary:
FUNCTION: SwissProt: Q6FHJ7 # Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
SIZE: 346 amino acids; 39827 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes.
DOMAIN:SwissProt: Q6FHJ7 The FZ domain is involved in binding with Wnt ligands (By similarity).
DISEASE:SwissProt: Q6FHJ7 # Associated with tumor-induced osteomalacia, a disorder in which there is an increase in renal phosphate excretion and a reduction in serum phosphate levels leading to hyperphosphaturia, hypophos -20℃