标签： sub

BML-SA131-0050   G protein α>sub>s subunit, pAb   品牌 Biomol

G protein α>sub>s subunit, pAb

货号： BML-SA131-0050      产品名称： G protein α>sub>s subunit, pAb   品牌： Biomol 规格： 50 ul 3周到货 生化实验

G protein αs subunit, pAb
Product Specification
Alternative Name:Gαs
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 385-394 of human Gαs subunit.
UniProt ID:P63092
Application:Immunoprecipitation
Western Blot
Specificity:Recognizes human and cow G protein (αs subunit).
Long Term Storage:-20°C

-20°C

06-238   A HU GBETA SUB.,RAB 50UL   品牌 Millipore

A HU GBETA SUB.,RAB 50UL

货号： 06-238      产品名称： A HU GBETA SUB.,RAB 50UL   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Gβ Antibody
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REFERENCES
Activation of p38 mitogen-activated protein kinase (p38 MAPK), extracellular signal-regulated kinase (ERK) and c-jun N-terminal kinase (JNK) during hypoxia in cerebral cortical nuclei of guinea pig fetus at term: role of nitric oxide.
Dev Maulik, Qazi M Ashraf, Om P Mishra, Maria Delivoria-Papadopoulos (2008) Neuroscience letters.439:94-9
Importance of the G protein gamma subunit in activating G protein-coupled inward rectifier K(+) channels.
T Kawano, L Chen, S Y Watanabe, J Yamauchi, Y Kaziro, Y Nakajima, S Nakajima, H Itoh (1999) FEBS letters.463:355-9
Differential regulation of mitogen-activated protein kinase kinase 4 (MKK4) and 7 (MKK7) by signaling from G protein beta gamma subunit in human embryonal kidney 293 cells.
J Yamauchi, Y Kaziro, H Itoh (1999) The Journal of biological chemistry.274:1957-65
Activation of p38 mitogen-activated protein kinase by signaling through G protein-coupled receptors. Involvement of Gbetagamma and Galphaq/11 subunits.
J Yamauchi, M Nagao, Y Kaziro, H Itoh (1997) The Journal of biological chemistry.272:27771-7
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Species Reactivity Key Applications Host Format Antibody Type
H, M  IP, WB Rabbit Serum Polyclonal Antibody
Description:
Anti-Gβ Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Gβ subunits; recognizes both isoforms
Molecular Weight:
35/36kDa
Immunogen:
Internal sequence of G-protein β subunit (KTREGNVRVSREL) conjugated to octavalent polylysine base
Isotype:
IgG
Species Reactivity:
Human
Mouse
Quality Assurance:
Immunoblot Analysis: A 1:500-1:1000 dilution of this antibody detected the Gβ subunits in 20 μg of a cell lysate prepared from rat brain microsomal preparation (Catalog # 12-144).
Immunoprecipitation/Immunoblot: 10 μl of this antibody immunoprecipitated the Gβ subunit from 500 μg-1mg of a lysate of rat brain microsomal preparation (Catalog # 12-144).
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Purification Method:
Antiserum
Presentation:
PBS, pH 7.2
Storage Conditions:
2 years at -20°C
UniProt Number:
P62879
Entrez Gene Number:
NM_002074
Gene Symbol:
GNB2
SG
IG
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene contains a trinucleotide (CCG) repeat length polymorphism in its 5′ UTR.
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UniProt Summary:
FUNCTION: SwissProt: P62879 # Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
SIZE: 340 amino aci -20°C

06-270   A HU ! GIA-3 SUB.,RAB 50UL   品牌 Millipore

A HU ! GIA-3 SUB.,RAB 50UL

货号： 06-270      产品名称： A HU ! GIA-3 SUB.,RAB 50UL   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Giα3 Antibody
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REFERENCES
Subcellular localization of LGN during mitosis: evidence for its cortical localization in mitotic cell culture systems and its requirement for normal cell cycle progression.
Rachna Kaushik, Fengwei Yu, William Chia, Xiaohang Yang, Sami Bahri (2003) Molecular biology of the cell.14:3144-55
G(o)-protein alpha-subunits activate mitogen-activated protein kinase via a novel protein kinase C-dependent mechanism.
van Biesen, T, et al. (1996) J. Biol. Chem., 271: 1266-9 (1996)
Guanine nucleotide binding regulatory proteins and adenylate cyclase in livers of streptozotocin- and BB/Wor-diabetic rats. Immunodetection of Gs and Gi with antisera prepared against synthetic peptides.
Lynch, C J, et al. (1989) J. Clin. Invest., 83: 2050-62 (1989)
Disulfide bonds in the extracellular calcium-polyvalent cation-sensing receptor correlate with dimer formation and its response to divalent cations in vitro
Ward, D. T., et al (1998) J Biol Chem, 273:14476-83 (1998)
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Species Reactivity Key Applications Host Format Antibody Type
H, Ht, M, R  IP, WB Rabbit Serum Polyclonal Antibody
Description:
Anti-Giα3 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Giα3 and Goα; low cross-reactivity with other G-proteins.
Molecular Weight:
40 kDa
Immunogen:
peptide corresponding to the C-terminal region of Giα3 (KNNLKECGLY)
Isotype:
IgG
Species Reactivity:
Human
Hamster
Mouse
Rat
Control:
Widely expressed
Quality Assurance:
routinely evaluated by immunoblot on rat hippocampus membrane preparation, solubilized rat and mouse brain membranes or mouse 3T3/A31 cell lysates
Purification Method:
Unpurified
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
P08754
Entrez Gene Number:
NM_006496
Gene Symbol:
GNAI3
87U6
G
FLJ26559
ialpha-3).
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P08754 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor- regulated K(+) channels.
SIZE: 354 amino acids; 40532 Da
SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity).
SIMILARITY: SwissProt: P08754 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.
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Brand Family:
Upstate
Product Name:
Anti-Giα3 Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
A -20°C

06-411   A MS P.K.ARII SUB PC12-305   品牌 Millipore

A MS P.K.ARII SUB PC12-305

货号： 06-411      产品名称： A MS P.K.ARII SUB PC12-305   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-PKA Antibody, RII Subunits
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REFERENCES
Myospryn is a calcineurin-interacting protein that negatively modulates slow-fiber-type transformation and skeletal muscle regeneration.
Ondra M Kielbasa,Joseph G Reynolds,Chia-Ling Wu,Christine M Snyder,Min Y Cho,Hartmut Weiler,Susan Kandarian,Francisco J Naya (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology.25
Calyculin A-sensitive protein phosphatases are involved in maintenance of progressive movement in mouse spermatozoa in vitro by suppression of autophosphorylation of protein kinase A.
Namiko Goto,Hiroshi Harayama (2009) The Journal of reproduction and development.55
Ultraviolet B-induced phosphorylation of histone H3 at serine 28 is mediated by MSK1.
Zhong, S, et al. (2001) J. Biol. Chem., 276: 33213-9 (2001)
Functional and subcellular changes in the A-kinase-signaling pathway: relation to aromatase and Sgk expression during the transition of granulosa cells to luteal cells.
I J Gonzalez-Robayna, T N Alliston, P Buse, G L Firestone, J S Richards (1999) Molecular endocrinology (Baltimore, Md.).13:1318-37
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Species Reactivity Key Applications Host Format Antibody Type
H, R  IP, WB, ICC Goat Purified Polyclonal Antibody
Description:
Anti-PKA Antibody, RII Subunits
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
RIIα and β subunits
Molecular Weight:
51/54kDa
Immunogen:
Recombinant RII subunits of mouse cAMP-dependent protein kinase expressed in E. coli
Isotype:
IgG
Species Reactivity:
Human
Rat
Quality Assurance:
routinely evaluated by immunoblot on mouse 3T3/A31 cells
Purification Method:
Protein G chromatography
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing 0.05% sodium azide
Storage Conditions:
2 years at -20°C
UniProt Number:
P13861
Entrez Gene Number:
NM_004157, NM_002736
Gene Symbol:
PRKAR2A
MGC3606
PKR2
PRKAR2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
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UniProt Summary:
FUNCTION: -20°C

06-727   A G SUB (TCR, FC REC) 200UG   品牌 Millipore

A G SUB (TCR, FC REC) 200UG

货号： 06-727      产品名称： A G SUB (TCR, FC REC) 200UG   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-FcεRI Antibody, γ subunit
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REFERENCES
FcγRI mediates serum amyloid P inhibition of fibrocyte differentiation.
Jeffrey R Crawford,Darrell Pilling,Richard H Gomer (2012) Journal of leukocyte biology.92
T cells stimulated with an analog peptide of type II collagen require the Fc receptor γ-chain to secrete interleukin-4 and suppress autoimmune arthritis in mice.
(2011) Arthritis and rheumatism.63:2661-70
2 integrin induces TCR-Syk-phospholipase C- phosphorylation and paxillin-dependent granule polarization in human NK cells.
Michael E March,Eric O Long (2011) Journal of immunology (Baltimore, Md. : 1950).186
The human megakaryocytic cell line UT-7/TPO expresses functional platelet agonist signals mediated through GPVI and thromboxane receptor.
Tatsuya Kawaguchi,Ryuji Hashimoto,Hiroshi Yokota (2010) Cell biology international.34
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IP Rabbit Purified Polyclonal Antibody
Description:
Anti-FcεRI Antibody, γ subunit
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes and is specific for FcεRI, γ-subunit, MW 10kDa.
Molecular Weight:
30 kDa
Immunogen:
Synthetic peptide KHEKPPQ corresponding to amino acids 80-86 of γ-subunit of Fc receptors.
Isotype:
IgG
Background Information:
Type I allergic reactions are mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for type I allergic reactions. The FceRI receptor is a tetrameric complex, consisting of an a-chain, b-chain and a dimeric g-chain. While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, rat and mouse. Other species cross-reactivity not tested.
Application Notes:
Immunoprecipitation: Reported to immunoprecipitate FcεR1, γ-subunit from RBL cells.
Control:
RBL cell lysate
Quality Assurance:
Evaluated by western blot on RIPA lysate of RBL cells.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FcεRI, γ-subunit in RIPA lysate of RBL cells.
Purification Method:
Protein A purfied
Presentation:
Purified rabbit polylclonal IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol.
Storage Conditions:
Stable for 1 year at from date of receipt.
UniProt Number:
P30273
Gene Symbol:
FCER1G
OTTHUMP00000063478
Alternate Names:
Fc fragment of IgE, high affinity I, receptor for, gamma polypeptide
Fc fragment of IgE, high affinity I, receptor for
gamma
polypeptide
Fc-epsilon RI-gamma
FceRI gamma
IgE Fc receptor subunit gamma
immunoglobulin E receptor, high affinity, gamma chain
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompany

07-250   ANTI-PP2A A SUB PC 12-305   品牌 Millipore

ANTI-PP2A A SUB PC 12-305

货号： 07-250      产品名称： ANTI-PP2A A SUB PC 12-305   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-PP2A Antibody, A subunit
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REFERENCES
Absence of PPP2R1A mutations in Wilms tumor.
Ruteshouser, E C, et al. (2001) Oncogene, 20: 2050-4 (2001)
Alterations in protein phosphatase 2A subunit interaction in human carcinomas of the lung and colon with mutations in the A beta subunit gene.
Ruediger, R, et al. (2001) Oncogene, 20: 1892-9 (2001)
Disruption of protein phosphatase 2A subunit interaction in human cancers with mutations in the A alpha subunit gene.
Ruediger, R, et al. (2001) Oncogene, 20: 10-5 (2001)
Low frequency of alterations of the alpha (PPP2R1A) and beta (PPP2R1B) isoforms of the subunit A of the serine-threonine phosphatase 2A in human neoplasms.
Calin, G A, et al. (2000) Oncogene, 19: 1191-5 (2000)
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Species Reactivity Key Applications Host Format Antibody Type
H, M, Po, R, Xn  IP, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-PP2A Antibody, A subunit
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
PP2A A subunit, α isoform
Molecular Weight:
63-65kDa
Immunogen:
KLH-conjugated, synthetic peptide of 13 amino acids (MAAADGDDSLY-Ahx-C), containing amino acids 1-11 of human PP2A, A subunit. Ahx is aminohexanoic acid. The immunizing sequence is conserved in human, mouse, rat, porcine and Xenopus.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Pig
Rat
Xenopus
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Quality Assurance:
Routinely evaluated by immunoblot on RIPA lysates from 3T3/A31 cells.
Purification Method:
Immunoaffinity chromatography
Presentation:
100mM triethylamine, 100mM sodium phosphate, 0.02% sodium azide before the addition of glycerol to 30%.
Storage Conditions:
1 year at -20°C
UniProt Number:
P30154
Entrez Gene Number:
NM_002716
Gene Symbol:
PPP2R1B
MGC26454
PP2A-A-beta
PR65B
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Defects in this gene could be the cause of some lung and colon cancers. At least two transcript variants encoding different isoforms have been found for this gene.
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UniProt Summary:
FUNCTION: SwissProt: P30154 # The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
SIZE: 601 amino acids; 66214 Da
SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa consta -20℃

MABN487   X-Cav3.2 Clcm Chnl Sub cl N55/10   品牌 Millipore

X-Cav3.2 Clcm Chnl Sub cl N55/10

货号： MABN487      产品名称： X-Cav3.2 Clcm Chnl Sub cl N55/10   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Cav3.2 Calcium Channel Subunit, clone N55/10
Species Reactivity Key Applications Host Format Antibody Type
M  WB Mouse Purified Monoclonal Antibody
Description:
Anti-Cav3.2 Calcium Channel Subunit Antibody, clone N55/10
Specificity:
This antibody does not cross react with human Cav3.1 Calcium Channel Subunit (Prof. J. Trimmer, University of California, Davis.).
Molecular Weight:
~260 kDa observed
Immunogen:
Recombinant protein corresponding to human Cav3.2 Calcium Channel Subunit.
Clone:
N55/10
Isotype:
IgG1κ
Background Information:
Voltage-gated calcium channel subunit alpha Cav3.2 is also called Voltage-dependent T-type calcium channel subunit alpha-1H and Low-voltage-activated calcium channel alpha 1 3.2 subunit. Voltage-gated calcium channel subunit alpha Cav3.2 and other voltage-sensitive calcium channels (VSCC) are involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. Voltage-gated calcium channel subunit alpha Cav3.2 is expressed in kidney, liver, heart, brain, and isoform 2 appears to be testis specific. Voltage-gated calcium channel subunit alpha Cav3.2 defects can increase susceptibility to epilepsy, idiopathic generalized type 6 (EIG6) and epilepsy, childhood absence type 6 (ECA6).
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Species Reactivity:
Mouse
Species Reactivity Note:
Demonstrated to react with Mouse. Predicted to react with human based upon immunogen design.
Application Notes:
Immunohistochemistry Analysis: A representative lot detected Cav3.2 Calcium Channel Subunit in wild type mice and demonstrated a loss of signal in Cav3.2 knock out mice (Prof. J. Trimmer, University of California, Davis.).
Western Blotting Analysis: A representative lot detected Cav3.2 Calcium Channel Subunit in HEK293 cell lysates expressing Flag-tagged Cav3.2 and demonstrated a loss of signal in HEK293 cell lysates expressing Flag-tagged Kv2.1 and Flag-tagged Cav3.1 (Prof. J. Trimmer, University of California, Davis.).
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Control:
NIH/3T3 cell lysate
Quality Assurance:
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Cav3.2 Calcium Channel Subunit in 10 µg of NIH/3T3 cell lysate.
Purification Method:
Protein G purified
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O95180
Entrez Gene Number:
NP_001005407
Gene Symbol:
CACNA1H
Alternate Names:
Cav3.2 Calcium Channel Subunit
Voltage-dependent T-type calcium channel subunit alpha-1H
Low-voltage-activated calcium channel alpha1 3.2 subunit
Voltage-gated calcium channel subunit alpha Cav3.2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Product Name:
Anti-Cav3.2 Calcium Channel Subunit, clone N55/10
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Form 2-8℃