ANTI-NIBRIN/NBS1 PC 12-303

货号： 05-616      产品名称： ANTI-NIBRIN/NBS1 PC 12-303   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Nibrin/Nbs1 Antibody, clone EE15
Species Reactivity Key Applications Host Format Antibody Type
H  IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-Nibrin/Nbs1 Antibody, clone EE15
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Nibrin/Nbs1
Molecular Weight:
95kDa
Immunogen:
GST fusion protein corresponding to residues 420-774 of human Nibrin/Nbs1
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot on Jurkat, HeLa nuclear extract, HeLa whole cell, and A431+/- EGF lysates
Purification Method:
Protein G chromatography
Presentation:
100mM Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
O60934
Entrez Gene Number:
NM_002485
Gene Symbol:
NBN
P95
MGC87362
Nibrin
FLJ10155
ATV
AT-V2
NBS
AT-V1
NBS1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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UniProt Summary:
FUNCTION: SwissProt: O60934 # Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3′-5′ exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3′ overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability.
SIZE: 754 amino acids; 84959 Da
SUBUNIT: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD -20°C

ANTI-NIBRIN/NBS1 50UL PC 12-303

货号： 07-317      产品名称： ANTI-NIBRIN/NBS1 50UL PC 12-303   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Nibrin/Nbs1 Antibody
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REFERENCES
The FANC pathway is activated by adenovirus infection and promotes viral replication-dependent recombination.
Gioia Cherubini,Valeria Naim,Paola Caruso,Romina Burla,Massimo Bogliolo,Enrico Cundari,Karim Benihoud,Isabella Saggio,Filippo Rosselli (2011) Nucleic acids research.39
BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.
Wang, Y, et al. (2000) Genes Dev., 14: 927-39 (2000)
ATM phosphorylates p95/nbs1 in an S-phase checkpoint pathway.
Lim, D S, et al. (2000) Nature, 404: 613-7 (2000)
The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response.
Carney, J P, et al. (1998) Cell, 93: 477-86 (1998)
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Species Reactivity Key Applications Host Format Antibody Type
H  IP, WB, ICC Rabbit Serum Polyclonal Antibody
Description:
Anti-Nibrin/Nbs1 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Nibrin/Nbs1
Molecular Weight:
95kDa
Immunogen:
GST fusion protein corresponding to residues 395-742 of human Nibrin/Nbs1/hp95
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot in Jurkat and A431 cell lysates
Purification Method:
Antiserum
Presentation:
antisera with 0.02% sodium azide
Storage Conditions:
1 year at 4°C
UniProt Number:
O60934
Entrez Gene Number:
NM_002485
Gene Symbol:
NBN
P95
FLJ10155
NBS
AT-V1
AT-V2
MGC87362
ATV
Nibrin
NBS1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
View All »
UniProt Summary:
FUNCTION: SwissProt: O60934 # Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3′-5′ exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proxim 4℃