标签归档：igfbp

407254-25UG   IGFBP-3, CF, Mouse, Recombinant   品牌 Merck

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IGFBP-3, CF, Mouse, Recombinant

货号： 407254-25UG      产品名称： IGFBP-3, CF, Mouse, Recombinant   品牌： Merck 规格： *** 3周到货 生化实验

IGFBP-3, CF, Mouse, Recombinant
Form  Solid
Formulation  Lyophilized from 0.2 µm filtered Acetonitrile, TFA.
Biological activity  ED50 = 0.125-0.5 µg/ml as measured by inhibition of the biological activity of IGF-I or IGF-II (30 ng/ml) on MCF-7 human breast adenocarcinoma cells.
Purity  ≥95% by SDS-PAGE
Contaminants  Endotoxin: <1.0 EU/µg protein
商店和运输信息
Storage  -20°C
Ship  Ambient Temperature Only
Standard Handling

-20°C

407255-25UG   IGFBP-rp1/IGFBP-7, His-Tag, CF, Mo., Rec   品牌 Merck

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IGFBP-rp1/IGFBP-7, His-Tag, CF, Mo., Rec

货号： 407255-25UG      产品名称： IGFBP-rp1/IGFBP-7, His-Tag, CF, Mo., Rec   品牌： Merck 规格： *** 3周到货 生化实验

IGFBP-rp1/IGFBP-7, His-Tag, CF, Mouse, Recombinant
Recombinant, mouse insulin-like growth factor binding protein-related protein 1 (IGFBP-rp1) consisting of amino acids 28-281 and expressed in S. frugiperda 21 cells with an N-terminal His•Tag®. Mouse IGFBP-rp1 cDNA encodes a 281 amino acid precursor protein which contains a putative 25 aa signal peptide and a 256 aa mature region. IGFBP modifies affinities of the binding proteins to IGF.
产品信息
Form  Solid
Formulation  Lyophilized from 0.2 µm sterile-filtered PBS.
Biological activity  Functional ELISA Immobilized rmIGFBP-rp1/IGFBP-7 (500 ng/ml) produces 50% optimal binding to rhCCL21/6Ckine at 4-20 ng/ml.
Purity  ≥97% by SDS-PAGE
Contaminants  Endotoxin: <1.0 EU per/1 µg protein
商店和运输信息
Storage  -20°C
Ship  Ambient Temperature Only
Standard Handling

-20°C

03-251   RIPAb+ IGF2BP2, clone 5E10-3E1   品牌 Millipore

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RIPAb+ IGF2BP2, clone 5E10-3E1

货号： 03-251      产品名称： RIPAb+ IGF2BP2, clone 5E10-3E1   品牌： Millipore 规格： EA 3周到货 生化实验

RIPAb+™ IGF2BP2 Antibody
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Species Reactivity Key Applications Host Format Antibody Type
H  WB, RIP Mouse Purified null
Description:
RIPAb+™ IGF2BP2 Antibody | 03-251
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Product Overview:
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA-binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
IGF2BP2 (Insulin-like growth factor 2 mRNA-binding protein 2) is a member of the IGF-II mRNA-binding protein family that post-transcriptionally regulates IGF2 during developmental stages. IGF2BP1, 2, and 3 bind the UTR region of IGF2 transcripts, and are implicated in the transport of RNA targets, to enable protein synthesis at specific locations in the cytoplasm. Research has shown that the IGF2 gene may be involved in ovarian cancer.
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Molecular Weight:
~66 kDa
Immunogen:
Histidine-tagged recombinant protein corresponding to human IGF2BP2
Isotype:
IgG1κ
Background Information:
Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a member of the IGF-II mRNA binding protein family that posttranscriptionally regulates IGF2 during developmental stages. IGF2 mRNA-binding protein family members (IGF2BP1, 2 and 3) bind UTR region of IGF2 transcripts and are implicated in the transport of RNA targets to enable protein synthesis at specific locations in the cytoplasm. Studies have indicated variations in the IGF2BP2 gene may affect fetal glucose metabolism and plays a role in type 2 diabetes. In addition to playing a role in glucose metabolism and dieabetes, the insulin-like growth factor (IGF) signaling axis plays an important role in cancer biology. Research has shown IGF2 to be emerging as an important gene for ovarian cancer.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react in human
Application Notes:
Western Blot Analysis:
Representative lot data.
HepG2 cell lysate was probed with Anti-IGF2BP2, clone 5E10-3E1 (1:20,000 dilution).
Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates IGF2BP2 (~66 kDa).
Note: An uncharacterized band appears at ~50 kDa in some lysates.
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Control:
Includes normal mouse IgG and primers specific for human IGF2 mRNA.
Quality Assurance:
RNA Binding Protein Immunoprecipitation:
Representative lot data
RIP Lysate prepared from 293 cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 1.25 µg of either a normal mouse IgG or 1.25 µg Anti-IGF2BP2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of IGF2BP2-associated RNA was verified by qPCR using RIP Primers IGF2, (Figure 1).
Please refer to the Magna RIP&tr -20°C

10430H07H25   RECOM HU IGFBP3 (N-HIS)   品牌 Invitrogen

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RECOM HU IGFBP3 (N-HIS)

货号： 10430H07H25      产品名称： RECOM HU IGFBP3 (N-HIS)   品牌： Invitrogen 规格： 25UG 三周到货 生化试验

IGFBP3 Recombinant Human Protein, His Tag
Insulin-like growth factor binding protein 3 (IGFBP3), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.
This recombinant protein was expressed from a DNA sequence encoding the mature form of human IGFBP3 (NP_000589.2), residues 28 to 291), fused to a polyhistidine tag at the N-terminus.
Activity: Measured by its binding ability in a functional ELISA.
1. Immobilized human IGF2 at 10 µg/ml(100 µl/well) can biotinylated human IGFBP3-His. The ED50 of can biotinylated human IGFBP3-His is 18 ng/ml. 2. Immobilized human IGF1 at 10 µg/ml(100 µl/well) can biotinylated human IGFBP3-His. The ED50 of can biotinylated human IGFBP3-His is 24 ng/ml.
Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Form: Lyophilized 
Purity: ≥ 85% by SDS-PAGE 
Gene ID: 3486 
Species: Human 
Expression: Mammalian 
Gene Symbol: IGFBP3 
Gene Aliases: IGFBP3, tcag7.703, BP-53, IBP3 
Product Size: 5 x 5 µg 
Protein Form: Recombinant 
Protein Family: Growth Factors & Receptors 
Endotoxin Level: < 1 EU/µg 
Protein Subtype: IGFs (Insulin-Like Growth Factors) 
Molecular Weight: 31 kDa 
Research Category: Development,
Apoptosis,
Neurobiology,
Oncology 
Protein Tag or Fusion: His Tag 
Validated Application: Assay Standard
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Store at -20 to -70 °C

10430H07H250   RECOM HU IGFBP3 (N-HIS)   品牌 Invitrogen

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RECOM HU IGFBP3 (N-HIS)

货号： 10430H07H250      产品名称： RECOM HU IGFBP3 (N-HIS)   品牌： Invitrogen 规格： 250UG 三周到货 生化试验

IGFBP3 Recombinant Human Protein, His Tag
Insulin-like growth factor binding protein 3 (IGFBP3), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.
This recombinant protein was expressed from a DNA sequence encoding the mature form of human IGFBP3 (NP_000589.2), residues 28 to 291), fused to a polyhistidine tag at the N-terminus.
Activity: Measured by its binding ability in a functional ELISA.
1. Immobilized human IGF2 at 10 µg/ml(100 µl/well) can biotinylated human IGFBP3-His. The ED50 of can biotinylated human IGFBP3-His is 18 ng/ml. 2. Immobilized human IGF1 at 10 µg/ml(100 µl/well) can biotinylated human IGFBP3-His. The ED50 of can biotinylated human IGFBP3-His is 24 ng/ml.
Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Form: Lyophilized
Purity: ≥ 85% by SDS-PAGE 
Gene ID: 3486 
Species: Human 
Expression: Mammalian 
Gene Symbol: IGFBP3 
Gene Aliases: IGFBP3, tcag7.703, BP-53, IBP3 
Product Size: 5 x 50 µg 
Protein Form: Recombinant 
Protein Family: Growth Factors & Receptors 
Endotoxin Level: < 1 EU/µg 
Protein Subtype: IGFs (Insulin-Like Growth Factors) 
Molecular Weight: 31 kDa 
Research Category: Development,
Apoptosis,
Neurobiology,
Oncology 
Protein Tag or Fusion: His Tag 
Validated Application: Assay Standard
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Store at -20 to -70 °C