Calpain Inhibitor II, ALLM
货号： 1835-25 产品名称： Calpain Inhibitor II, ALLM 品牌： Biovision 规格： 25 mg 3周到货 生化实验
Calpain Inhibitor II, ALLM
A Calpain inhibitor
Product Overview
Product Name: Calpain Inhibitor II, ALLM
Alternate Name/Synonyms: Ac-LLM-CHO, N-Acetyl-Leu-Leu-Methional
Description: A cell-permeable inhibitor of calpain 1 (Ki = 120 nM) and calpain 2 (Ki = 230 nM). Also potently inhibits cathepsin B (Ki = 100 nM), and cathepsin L (Ki = 0.6 nM). Shown to induce caspase-dependent apoptosis in human acute lymphoblastic leukemia and non-Hodgkin’s Lymphoma cells.
Peptide Sequence: N-Acetyl-Leu-Leu-Methional
Appearance: White powder
Formulation: N/A
CAS Number: 136632-32-1
Molecular Formula: C₁₉H₃₅N₃O₄S
Molecular Weight: 401.57
Purity: ≥95% by HPLC
Solubility:DMSO (25 mg/ml) or EtOH (25 mg/ml)
Storage Temp.: -20°C
Shipping Conditions: gel pack
Handling: Protect from moisture and air
SMILES: CC(C)CC(C(=O)NC(CC(C)C)C(=O)NC(CCSC)C=O)NC(=O)C
InChi: InChI=1S/C19H35N3O4S/c1-12(2)9-16(20-14(5)24)19(26)22-17(10-13(3)4)18(25)21-15(11-23)7-8-27-6/h11-13,15-17H,7-10H2,1-6H3,(H,20,24)(H,21,25)(H,22,26)
InChi Key: RJWLAIMXRBDUMH-UHFFFAOYSA-N
PubChem CID: 4331
MDL Number: MFCD00065506
USAGE: For Research Use Only! Not For Use in Humans.
-20°C
BML-CG1920-0100 Calpain I, II small subunit p30, mAb (28F3) 品牌 Biomol
Calpain I, II small subunit p30, mAb (28F3)
货号： BML-CG1920-0100 产品名称： Calpain I, II small subunit p30, mAb (28F3) 品牌： Biomol 规格： 100 ug 3周 生化实验
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I) are composed of two subunits (80 kDa and 30 kDa). Whereas the small subunit is common to both enzymes, the larger catalytic subunits are different and exhibit distinct calcium ion requirements. m-Calpain requires millimolar levels of Ca2+, and μ-calpain is active at micromolar concentrations of Ca2+. In addition to the ubiquitously expressed m- and μ-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles.
Product Specification
Alternative Name:m-Calpain and μ-Calpain
Clone:28F3
Host:Mouse
Isotype:IgG1
Immunogen:Human placenta calpain
UniProt ID:P07384 (calpain I), P17655 (calpain II)
Application:ELISA
Immunoprecipitation
Western Blot
Specificity:Recognizes human and cow calpain I, II (small subunit p30).
Purity Detail:Chromatographically purified.
Formulation:Liquid. In buffer.
Long Term Storage:-20°C
-20°C
BML-CG1925-0500 Calpain I, II small subunit p30, mAb (28F3) (agarose immobilized) 品牌 Biomol
Calpain I, II small subunit p30, mAb (28F3) (agarose immobilized)
货号： BML-CG1925-0500 产品名称： Calpain I, II small subunit p30, mAb (28F3) (agarose immobilized) 品牌： Biomol 规格： 0.5 mL 3周 生化实验
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I) are composed of two subunits (80 kDa and 30 kDa). Whereas the small subunit is common to both enzymes, the larger catalytic subunits are different and exhibit distinct calcium ion requirements. m-Calpain requires millimolar levels of Ca2+, and μ-calpain is active at micromolar concentrations of Ca2+. In addition to the ubiquitously expressed m- and μ-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles.
Product Specification
Alternative Name:m-Calpain and μ-Calpain
Clone:28F3
Host:Mouse
Isotype:IgG1
Immunogen:Human placenta calpain
UniProt ID:P07384 (calpain I), P17655 (calpain II)
Application:ELISA
Immunoprecipitation
Western Blot
Specificity:Recognizes human and cow calpain I, II (small subunit p30).
Purity Detail:Chromatographically purified.
Formulation:Liquid. In buffer.
Long Term Storage:-20°C
-20°C
BML-CG1928-0100 Calpain I, mAb (15C10) 品牌 Biomol
Calpain I, mAb (15C10)
货号： BML-CG1928-0100 产品名称： Calpain I, mAb (15C10) 品牌： Biomol 规格： 100 ug 3周 生化实验
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I) are composed of two subunits (80 kDa and 30 kDa). Whereas the small subunit is common to both enzymes, the larger catalytic subunits are different and exhibit distinct calcium ion requirements. m-Calpain requires millimolar levels of Ca2+, and μ-calpain is active at micromolar concentrations of Ca2+. In addition to the ubiquitously expressed m- and μ-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles.
Product Specification
Alternative Name:μ-Calpain
Clone:15C10
Host:Mouse
Isotype:IgG1
Immunogen:Human placenta calpain.
UniProt ID:P07384
Application:ELISA
Immunoprecipitation
Western Blot
Specificity:Recognizes human, mouse, rat and cow calpain I.
Purity Detail:Chromatographically purified.
Formulation:Liquid. In 20mM sodium phosphate, 150mM sodium chloride, pH 7.5, containing 50% glycerol and 3mM sodium azide.
Long Term Storage:-20°C
-20°C
AB81011 CALPAIN LP85 & LP82, RBX-100UG 品牌 Millipore
CALPAIN LP85 & LP82, RBX-100UG
货号： AB81011 产品名称： CALPAIN LP85 & LP82, RBX-100UG 品牌： Millipore 规格： EA
AB81018 CALPAIN-94, INSERT 1, RBX-100UG 品牌 Millipore
CALPAIN-94, INSERT 1, RBX-100UG
货号： AB81018 产品名称： CALPAIN-94, INSERT 1, RBX-100UG 品牌： Millipore 规格： EA
AB81019 CALPAIN-94, INSERT 2, RBX-100UG 品牌 Millipore
CALPAIN-94, INSERT 2, RBX-100UG
货号： AB81019 产品名称： CALPAIN-94, INSERT 2, RBX-100UG 品牌： Millipore 规格： EA
AB81023 anti-Calpain-2 RBX 100 ul 品牌 Millipore
anti-Calpain-2 RBX 100 ul
货号： AB81023 产品名称： anti-Calpain-2 RBX 100 ul 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-Calpain II Antibody, large subunit
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REFERENCES
Brain-derived neurotrophic factor and epidermal growth factor activate neuronal m-calpain via mitogen-activated protein kinase-dependent phosphorylation.
Sohila Zadran,Hussam Jourdi,Karoline Rostamiani,Qingyu Qin,Xiaoning Bi,Michel Baudry (2010) The Journal of neuroscience : the official journal of the Society for Neuroscience.30
Species Reactivity Key Applications Host Format Antibody Type
R, M, H WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Calpain II Antibody, large subunit
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Trade Name:
Chemicon (Millipore)
Specificity:
The anti-calpain II antibody recognizes the 80 kDa and 58 kDa forms of Calpain II. It does not cross-react with other calpains.
Epitope:
large subunit
Background Information:
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF-hand domain IV.
Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
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Species Reactivity:
Rat
Mouse
Human
Application Notes:
Western blot: 1:1000 for colorimetric substrates or 1:5000 for chemiluminescent substrates (recommended starting concentration).
Optimal working dilutions must be determined by the end user.
Presentation:
Affinity purified immunoglobulin. Liquid in PBS pH 7.4 containing 0.02% sodium azide, 1% BSA and 50% glycerol as a stabilizer.
Storage Conditions:
Maintain at -20*C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
P17655
Entrez Gene Number:
NM_001748.4
Gene Symbol:
CAPN2
millimolar-calpain
M-calpain
Millimolar-calpain
mCANP
FLJ39928
CANP2
CANPml
CANPL2
EC 3.4.22.53
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Alternate Names:
M-Calpain; Calpain 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5′ UTR have been reported.
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UniProt Summary:
FUNCTION: SwissProt: P17655 # Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
COFACTOR: Binds 3 calcium ions.
SIZE: 700 amino acids; 80009 Da
SUBUNIT: Forms a heterodimer with a small (regulatory) subunit (CAPNS1).
SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca -20℃