标签归档：calpain

QIA120-1KIT   CALPAIN ACTIVITY ASSAY KIT, FLUOROGENIC   品牌 Merck

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CALPAIN ACTIVITY ASSAY KIT, FLUOROGENIC

货号： QIA120-1KIT      产品名称： CALPAIN ACTIVITY ASSAY KIT, FLUOROGENIC   品牌： Merck 规格： *** 3周到货 生化实验

Calpain Activity Assay Kit, Fluorogenic
Calpains are Ca2+-dependent proteinases, which are involved in normal cellular processes including cell proliferation, apoptosis, differentiation, and cell migration. Requires a fluorimeter or microplate reader capable of measuring fluorescence at wavelengths of Excitation max: ~360 nm and Emission max: ~460 nm.
产品信息
Format  96-well plate
Form  96 Tests
Detection method  Fluorometric
Protect from Light  是的
Avoid freeze/thaw  是的
Assay time  1.5 h
Sample type  Cell lysates, plasma, serum
Positive control  Calpain 1
Kit contains  Calibration Standard, Positive Control Calpain 1, Substrate, Activation Buffer, Inhibition Buffer, Reduction Agent, Assay Buffer, Cell Lysis Buffer, 96-Well Plate, Plate Sealer, and a user protocol.
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage  Multiple storage requirements
Ship  Multiple Storage Conditions
Multiple Toxicity Values, refer to MSDS

BML-SA255-0100   m-Calpain (domain III/IV), mAb (107-82)   品牌 Biomol

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m-Calpain (domain III/IV), mAb (107-82)

货号： BML-SA255-0100      产品名称： m-Calpain (domain III/IV), mAb (107-82)   品牌： Biomol 规格： 100 ul 3周到货 生化实验

m-Calpain (domain III/IV), mAb (107-82)
Product Specification
Alternative Name:Calpain II
Clone:107-82
Host:Mouse
Isotype:IgG1
Immunogen:Purified bovine skeletal muscle m-calpain.
Application:Immunocytochemistry
Western Blot
Specificity:Recognizes human, rat and pig m-calpain.
Formulation:Liquid. Ascites fluid.
Long Term Storage:-20°C

-20°C

1138-100   Active Porcine Calpain 1   品牌 Biovision

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Active Porcine Calpain 1

货号： 1138-100      产品名称： Active Porcine Calpain 1   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Active Porcine Calpain 1
A cysteine protease involved in apoptosis activation
Product Overview
Product Name: Active Porcine Calpain 1
Alternate Name/Synonyms: Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Micromolar-calpain
Gene Symbol: CAPN1
Accession #: P35750
Gene ID: 397027
Source: Porcine Erythrocytes
Appearance: Liquid
Physical Form Description: In 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
Molecular Weight: 110 kDa
Purity by SDS-PAGE: ≥85%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: ≥ 1000 units/mg protein prior to freezing.
Reconstitution Instructions: N/A
Storage Temp.: -70°C
Shipping: Gel pack
Background Information: The activity of Calpain activity is attributed to two main isoforms: n-calpain and m-calpain, which are ubiquitously, expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (n-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-70°C

K244-100   Human Calpain 1 Inhibitor Screening Kit   品牌 Biovision

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Human Calpain 1 Inhibitor Screening Kit

货号： K244-100      产品名称： Human Calpain 1 Inhibitor Screening Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

Human Calpain 1 Inhibitor Screening Kit (Fluorometric)
Product Overview
Assay Kit Name:
Human Calpain 1 Inhibitor Screening Kit
Kit Summary:
• Detection method- Fluorescence (Ex/Em= 400/505 nm)
• Species reactivity- Mammalian
• Applications- The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Calpain 1 inhibitors. Inhibitor control (Calpain inhibitor 1, ALLN) is included to compare the efficacy of test inhibitors.
Sample Type:
N/A
Features & Benefits:
• Simple procedure
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable
Kit components:
• Assay Buffer
• Substrate
• Calpain 1 Enzyme
• Inhibitor Control (Calpain Inhibitor 1, ALLN) [1 μM]
Description:
Calpain 1 is calcium-dependent, non-lysosomal cysteine protease with low calcium requirement. It is expressed ubiquitously and in the brain, mainly localized to the cell body and dendrites of neurons and to a lesser extent in axons and glial cells. Hyperactivation of calpains is implicated in a number of pathologies associated with altered calcium homeostasis such as Alzheimer’s disease as well as secondary degeneration resulting from acute cellular stress following cerebral (neuronal) ischemia, traumatic brain injury and spinal cord injury. BioVision’s human calpain 1 inhibitor screening kit is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC possess very weak fluorescence; upon cleavage of the substrate by calpain 1, free AFC can be quantified fluorometrically (Ex/Em = 400/505 nm). In the presence of potent Calpain 1 inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Calpain 1 inhibitors. Inhibitor control (Calpain inhibitor 1, ALLN) is included to compare the efficacy of test inhibitors.
Storage Conditions:
-80°C
Shipping Conditions:
Dry ice
USAGE: For Research Use Only! Not For Use in Humans.

-80°C

3372BP-50   Calpain-2 Blocking Peptide   品牌 Biovision

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Calpain-2 Blocking Peptide

货号： 3372BP-50      产品名称： Calpain-2 Blocking Peptide   品牌： Biovision 规格： 50 μg 3周到货 生化实验

Calpain-2 Blocking Peptide
Product Overview
Product Name: Calpain-2 Blocking Peptide
Antibody Target: Synaptotagmin
Accession #: P21707
Gene ID: 25716
Appearance: Colorless liquid
Concentration: 0.5mg/ml
Formulation: 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Storage Temp.: -20°C
Shipping: Gel Pack
Application & Usage: The peptide is used for blocking the antibody activity of synaptotagmin (Cat. # 3177-100). It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Description: 50 ug blocking peptide for synaptotagmin polyclonal Antibody (Cat. # 3177-100).
Handling: The peptide solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

AB1625   CALPAIN II LARGE SUBUNIT,RB X-100UL   品牌 Millipore

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CALPAIN II LARGE SUBUNIT,RB X-100UL

货号： AB1625      产品名称： CALPAIN II LARGE SUBUNIT,RB X-100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Calpain II Antibody, large (catalytic) subunit
Close
REFERENCES
Calpain system protein expression in basal-like and triple-negative invasive breast cancer.
S J Storr,K W Lee,C M Woolston,S Safuan,A R Green,R D Macmillan,A Benhasouna,T Parr,I O Ellis,S G Martin (2012) Annals of oncology : official journal of the European Society for Medical Oncology / ESMO.23
Abundance of calpain and aggrecan-cleavage products of calpain in degenerated human intervertebral discs.
S Fukuta,K Miyamoto,K Suzuki,H Maehara,T Inoue,K Kikuike,K Shimizu,A Hara,A Taguchi (2011) Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society.19
Role of mu-calpain in human decidua for recurrent miscarriage.
Kyoko Kumagai,Yasuhiko Ozaki,Tamao Nakanishi,Mitsushi Inomata,Tadahide Furuno,Mamoru Nakanishi,Mayumi S Ogasawara (2008) American journal of reproductive immunology (New York, N.Y. : 1989).59
Integrin alpha2-mediated ERK and calpain activation play a critical role in cell adhesion and motility via focal adhesion kinase signaling: identification of a novel signaling pathway
Sawhney, Rajinder S, et al (2006) J Biol Chem, 281:8497-510 (2006)
View All
Species Reactivity Key Applications Host Format Antibody Type
H  ELISA, WB, ICC, IHC Rabbit Serum Polyclonal Antibody
Description:
Anti-Calpain II Antibody, large (catalytic) subunit
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
Reacts with m-calpain (Calpain II) large subunit.
Epitope:
large (catalytic) subunit
Immunogen:
Human placental m-calpain.
Species Reactivity:
Human
Application Notes:
Immunoblot: 1:100
ELISA: 1:6,000 (against the immunogen)
Immunohistochemistry: 1:100 on 4% paraformaldehyde fixed tissue.
Immunocytochemistry. Tested positive on the following human cells: A431 epidermal carcinoma, MDB-MB-134-VI ductal breast carcinoma, C-33A cervical carcinoma, TE2 esophageal carcinoma, HET-1A esophageal carcinoma and normal human monocytes.
Optimal working dilutions must be determined by the end user.
View All »
Presentation:
Rabbit antiserum absorbed with human m-calpain bound to Sepharose™. Liquid. Contains no preservative.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
P17655
Entrez Gene Number:
NM_001748.4
Gene Symbol:
CAPN2
millimolar-calpain
M-calpain
Millimolar-calpain
mCANP
FLJ39928
CANP2
CANPml
CANPL2
EC 3.4.22.53
View All »
Alternate Names:
m-Calpain; Calpain 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
ELISA
Western Blotting
Immunocytochemistry
Immunohistochemistry
Entrez Gene Summary:
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterog -20℃

1834-5   Calpain Inhibitor I, ALLN   品牌 Biovision

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Calpain Inhibitor I, ALLN

货号： 1834-5      产品名称： Calpain Inhibitor I, ALLN   品牌： Biovision 规格： 5 mg 3周到货 生化实验

Calpain Inhibitor I, ALLN
A Calpain inhibitor
Product Overview
Product Name: Calpain Inhibitor I, ALLN
Alternate Name/Synonyms: Ac-LLnL-CHO, MG-101, N-Acetyl-L-leucyl-L-leucyl-L-norleucinal, N-Acetyl-Leu-Leu-Nle-al
Description: A cell-permeable inhibitor of calpain I (Ki = 190 nM), calpain II (Ki = 220 nM), cathepsin B (Ki = 150 nM), and cathepsin L (Ki = 0.5 nM). Inhibits neutral cysteine proteases and the proteasome (Ki = 6 µM). Can initiate apoptosis in HL-60 cells but blocks dexamethasone-induced apoptosis in thymocytes and cycloheximide-induced apoptosis in metamyelocytes. Inhibits cyclin B degradation and arrests the cell cycle at G1/S and at meta-/anaphase.
Peptide Sequence: Ac-Leu-Leu-Nile-CHO
Appearance: White powder
Formulation: N/A
CAS Number: 110044-82-1
Molecular Formula: C₂₀H₃₇N₃O₄
Molecular Weight: 383.54
Purity: ≥95% by HPLC
Solubility:DMSO (25 mg/ml) or EtOH
Storage Temp.: -20°C
Shipping Conditions: gel pack
Handling: Protect from moisture and air
SMILES: CCCCC(C=O)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)C
InChi: InChI=1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1
InChi Key: FMYKJLXRRQTBOR-BZSNNMDCSA-N
PubChem CID: 443118
MDL Number: MFCD00065505
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

1834-25   Calpain Inhibitor I, ALLN   品牌 Biovision

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Calpain Inhibitor I, ALLN

货号： 1834-25      产品名称： Calpain Inhibitor I, ALLN   品牌： Biovision 规格： 25 mg 3周到货 生化实验

Calpain Inhibitor I, ALLN
A Calpain inhibitor
Product Overview
Product Name: Calpain Inhibitor I, ALLN
Alternate Name/Synonyms: Ac-LLnL-CHO, MG-101, N-Acetyl-L-leucyl-L-leucyl-L-norleucinal, N-Acetyl-Leu-Leu-Nle-al
Description: A cell-permeable inhibitor of calpain I (Ki = 190 nM), calpain II (Ki = 220 nM), cathepsin B (Ki = 150 nM), and cathepsin L (Ki = 0.5 nM). Inhibits neutral cysteine proteases and the proteasome (Ki = 6 µM). Can initiate apoptosis in HL-60 cells but blocks dexamethasone-induced apoptosis in thymocytes and cycloheximide-induced apoptosis in metamyelocytes. Inhibits cyclin B degradation and arrests the cell cycle at G1/S and at meta-/anaphase.
Peptide Sequence: Ac-Leu-Leu-Nile-CHO
Appearance: White powder
Formulation: N/A
CAS Number: 110044-82-1
Molecular Formula: C₂₀H₃₇N₃O₄
Molecular Weight: 383.54
Purity: ≥95% by HPLC
Solubility:DMSO (25 mg/ml) or EtOH
Storage Temp.: -20°C
Shipping Conditions: gel pack
Handling: Protect from moisture and air
SMILES: CCCCC(C=O)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)C
InChi: InChI=1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1
InChi Key: FMYKJLXRRQTBOR-BZSNNMDCSA-N
PubChem CID: 443118
MDL Number: MFCD00065505
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

1835-5   Calpain Inhibitor II, ALLM   品牌 Biovision

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Calpain Inhibitor II, ALLM

货号： 1835-5      产品名称： Calpain Inhibitor II, ALLM   品牌： Biovision 规格： 5 mg 3周到货 生化实验

Calpain Inhibitor II, ALLM
A Calpain inhibitor
Product Overview
Product Name: Calpain Inhibitor II, ALLM
Alternate Name/Synonyms: Ac-LLM-CHO, N-Acetyl-Leu-Leu-Methional
Description: A cell-permeable inhibitor of calpain 1 (Ki = 120 nM) and calpain 2 (Ki = 230 nM). Also potently inhibits cathepsin B (Ki = 100 nM), and cathepsin L (Ki = 0.6 nM). Shown to induce caspase-dependent apoptosis in human acute lymphoblastic leukemia and non-Hodgkin’s Lymphoma cells.
Peptide Sequence: N-Acetyl-Leu-Leu-Methional
Appearance: White powder
Formulation: N/A
CAS Number: 136632-32-1
Molecular Formula: C₁₉H₃₅N₃O₄S
Molecular Weight: 401.57
Purity: ≥95% by HPLC
Solubility:DMSO (25 mg/ml) or EtOH (25 mg/ml)
Storage Temp.: -20°C
Shipping Conditions: gel pack
Handling: Protect from moisture and air
SMILES: CC(C)CC(C(=O)NC(CC(C)C)C(=O)NC(CCSC)C=O)NC(=O)C
InChi: InChI=1S/C19H35N3O4S/c1-12(2)9-16(20-14(5)24)19(26)22-17(10-13(3)4)18(25)21-15(11-23)7-8-27-6/h11-13,15-17H,7-10H2,1-6H3,(H,20,24)(H,21,25)(H,22,26)
InChi Key: RJWLAIMXRBDUMH-UHFFFAOYSA-N
PubChem CID: 4331
MDL Number: MFCD00065506
USAGE: For Research Use Only! Not For Use in Humans.

-20°C