p-ITCH (pT222), Rbx-100ug

货号： AB10050      产品名称： p-ITCH (pT222), Rbx-100ug   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-ITCH (pT222) Antibody
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REFERENCES
Jun turnover is controlled through JNK-dependent phosphorylation of the E3 ligase Itch.
Gao, Min, et al. (2004) Science, 306: 271-5 (2004)
The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover.
Chang L, Kamata H, Solinas G, Luo JL, Maeda S, Venuprasad K, Liu YC, Karin M. (2006) Cell, 123(3):601-13 (2006)
Activation of the E3 ubiquitin ligase Itch through a phosphorylation-induced conformational change.
Gallagher E, Gao M, Liu YC, Karin M. (2006) Proc Natl Acad Sci U S A. , 6:1717-1722 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H  DB, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-ITCH (pT222) Antibody
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Trade Name:
Chemicon (Millipore)
Specificity:
Human ITCH phosphorylated on threonine 222 (T222).
Immunogen:
Synthetic peptide corresponding to a portion of human ITCH encompassing the phosphorylated Threonine at position 222 (PPPpTPRRP).
Modifications:
Phosphorylation
Background Information:
ITCH is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. ITCH regulates the transcriptional activity of several transcription factors including the hematopoietic transcription factor NF-E2, and may play an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and also interacts with NOTCH1, OCLN, JUN, and JUNB. ITCH has a wide tissue distribution and is localized within the nucleus and cytoplasm. Two ITCH splice variants have been described; an approximately 103 kDa form as well as a truncated form (~98 kDa) lacking 42 amino acids in the N-terminal half of the protein. ITCH contains a MAP Kinase binding site and its activity is modulated by phosphorylation at multiple threonine residues by JNK1.
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Species Reactivity:
Human
Application Notes:
Western Blot: Recommended Dilution 1 – 5 μg/mL
Dot Blot
Presentation:
Affinity purified immunoglobulin. Liquid in Tris-citrate/phosphate buffer, pH 7-8, containing 0.1% sodium azide.
Storage Conditions:
Maintain at 2-8°C for up to 12 months from date of receipt
UniProt Number:
Q96J02
Entrez Gene Number:
NM_031483.3
Gene Symbol:
ITCH
NAPP1
dJ468O1.1
Itch
AIF4
AIP4
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Alternate Names:
Itchy homolog E3 ubiquitin protein ligase; AIP4; NAPP1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Dot Blot
Western Blotting
Entrez Gene Summary:
Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains a 2-8℃