标签： intracellular

22810   Cell Meter Intracellular GSH Assay Kit *Optimized for Flow Cytometry*   品牌 AAT

Cell Meter Intracellular GSH Assay Kit *Optimized for Flow Cytometry*

货号： 22810      产品名称： Cell Meter Intracellular GSH Assay Kit *Optimized for Flow Cytometry*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Meter� Intracellular GSH Assay Kit *Optimized for Flow Cytometry*
Cat # 22810
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 490
Em (nm) 515 
Our Cell Meter� assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the depleted antioxidant reduced glutathione (GSH). GSH is involved in many cellular processes including the scavenging of free radicals, drug detoxification, cell signaling, and cell proliferation. The decrease in cellular GSH concentration is an early hallmark in the progression of cell death in response to different apoptotic stimuli in many cell types. Our Cell Meter� Intracellular GSH Assay Kit provides all the essential components with an optimized assay method for the detection of apoptosis in cells with the decreased GSH. This fluorometric assay is based on the detection of the GSH in cells using our proprietary non-fluorescent Thiolite� Green dye that becomes strongly fluorescent upon reacting with thiol. In normal cells, Thiolite� Green accumulates primarily in cytosol, but it is partially translocated to mitochondria in apoptotic cells while Thiolite� Green staining intensity decreases. Cells stained with Thiolite� Green can be visualized by flow cytometry with Ex/Em = 490 nm/520 nm (FL1 channel). The kit can be paired with other reagents, such as 7-AAD (Cat# 17501), propidium iodide (Cat# 17517) for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening of apoptosis activators and inhibitors by flow cytometry.
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Cell Cytotoxicity     Apoptosis and Cytotoxicity     Cell Apoptosis 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

22900   Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*   品牌 AAT

Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*

货号： 22900      产品名称： Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Meter� Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*
Cat # 22900
Storage F/D/L (see storage codes below)
Unit Size 200 Tests
Ex (nm) 492
Em (nm) 520  
Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways. Cell Meter� Fluorimetric ROS Assay Kit uses our unique ROS sensor to quantify ROS in live cells. ROS Green is cell-permeable. It generates the green fluorescence when it reacts with ROS. The kit is an optimized 搈ix and read� assay format that is compatible with HTS liquid handling instruments. The Cell Meter� Fluorimetric ROS Assay Kit provides a sensitive, one-step fluorimetric assay to detect intracellular ROS in live cells with one hour incubation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read using either a fluorescence microplate reader or a fluorescence microscope at Ex/Em = 490/520 nm.  
Detailed Information:   Protocol      References      MSDS
Related Products:     Reactive Oxygen Species     Cell Signaling     Cell Functional Analysis 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

22901   Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit*Orange Fluorescence*   品牌 AAT

Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit*Orange Fluorescence*

货号： 22901      产品名称： Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit*Orange Fluorescence*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Meter� Fluorimetric Intracellular Total ROS Activity Assay Kit*Red Fluorescence*
Cat # 22901
Storage F/D/L (see storage codes below)
Unit Size 200 Tests
Ex (nm) 520
Em (nm) 605 
Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways. Amplite� Fluorimetric ROS Assay Kit uses our unique ROS sensor to quantify ROS in live cells. Amplite� ROS Red is cell-permeable. It generates the red fluorescence when it reacts with ROS. The kit is an optimized 搈ix and read� assay format. The Amplite� Fluorimetric ROS Assay Kit provides a sensitive, one-step fluorimetric assay to detect intracellular ROS in live cells with 1-2 hours incubation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read using either a fluorescence microplate reader or a fluorescent microscope at Ex/Em = 520/605 nm. It can be used to either quantify the ROS activities or screen the ROS inhibitors.  
Detailed Information:   Protocol      References      MSDS
Related Products:     Reactive Oxygen Species     Cell Signaling     Cell Functional Analysis 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

4414155   HUMAN INTRACELLULAR CALCIUM   品牌 Invitrogen

HUMAN INTRACELLULAR CALCIUM

货号： 4414155      产品名称： HUMAN INTRACELLULAR CALCIUM   品牌： Invitrogen 规格： SIGNALING KIT 三周到货 生化试验

TaqMan® Array Human Intracellular Calcium Signaling
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Intracellular Calcium Signaling 96-well Plate contains 92 assays to genes associated with intracellular calcium signaling and 4 assays to candidate endogenous control genes.
Form: Dried Down
Format: 96-well plate 
Species: Human 
Label or Dye: FAM™
Product Size: 1 standard plate 
Reaction Speed: Standard 
Research Category: Cell Cycle & Proliferation,
Extracellular Matrix & Cell Adhesion,
Oncology,
Immunology,
Neurobiology,
Signal Transduction,
Development,
Reproduction
Internal Probe Modification: Non-Fluorescent Quencher (3′),
MGB (Minor Groove Binder) (3′),
FAM (5′) 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Each TaqMan® Array Plate well contains a single TaqMan® Assay for one 20 µL reaction. The plates can be stored at room temperature or at 4°C.

4418800   HS INTRACELLULAR CALCIUM   品牌 Invitrogen

HS INTRACELLULAR CALCIUM

货号： 4418800      产品名称： HS INTRACELLULAR CALCIUM   品牌： Invitrogen 规格： SIGNALING FAST KIT 三周到货 生化试验

TaqMan® Array, Human Intracellular Calcium Signaling, Fast 96-well
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Intracellular Calcium Signaling 96-well Plate contains 92 assays to genes associated with intracellular calcium signaling and 4 assays to candidate endogenous control genes.
Form: Dried Down 
Format: 96-well plate 
Species: Human 
Label or Dye: FAM™ 
Product Size: 1 x 96 well plate 
Reaction Speed: Fast 
Research Category: Biomarker-Related Pathway(s),
Extracellular Matrix & Cell Adhesion,
Oncology,
Development,
Cell Cycle & Proliferation
Internal Probe Modification: Non-Fluorescent Quencher (3′),
MGB (Minor Groove Binder) (3′),
FAM (5′)
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Each TaqMan® Array Plate well contains a single TaqMan® Assay for one 10 µL reaction. The plates can be stored at room temperature or at 4°C.

07-1231   Anti-Notch 1, Intracellular (rabbit polyclonal)   品牌 Millipore

Anti-Notch 1, Intracellular (rabbit polyclonal)

货号： 07-1231      产品名称： Anti-Notch 1, Intracellular (rabbit polyclonal)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Notch1 Antibody, Intracellular
Recommended Replacement for: 07-1231 is a recommended replacement for AB5707
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PRODUCT FAMILY INFORMATION
Glial Cell Markers
Glial cells, sometimes called neuroglia are non-neuronal cells that maintain homeostasis, form myelin, and provide support and protection for neurons in the brain and peripheral nervous system.
Glial cells, sometimes called neuroglia are non-neuronal cells that maintain homeostasis, form myelin, and provide support and protection for neurons in the brain and peripheral nervous system.
» More…
Species Reactivity Key Applications Host Format Antibody Type
H  ELISA, WB, IH(P) Rabbit Serum Polyclonal Antibody
Description:
Anti-Notch1 Antibody, Intracellular
Replaces:
07-1231 is a recommended replacement for AB5707
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
Notch 1, intracellular. No reactivity against human Notch 2.
Molecular Weight:
~80 kDa
Epitope:
Intracellular
Immunogen:
Synthetic peptide from human Notch 1.
Isotype:
IgG
Background Information:
Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Human. No reactivity observed to mouse Notch. Reactivity with other species has not been determined.
Application Notes:
ELISA: 1:20,000-1:100,000 dilution from a previous lot was used in a standard sandwich ELISA assay against the peptide immunogen.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining IGF-1 With Epitope Retrieval: Lipoma.
View All »
Control:
HeLa Cell Lysate
Quality Assurance:
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected NOTCH1, intracellular (Cleaved form) on 10 μg of HeLa lysates.
Presentation:
Rabbit polyclonal IgG serum in buffer containing in 0.02 M Potassium phosphate, 0.15 M NaCl with 0.01% sodium azide.
Storage Conditions:
Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
-20°C

07-1232   Anti-Notch 1, Notch Intracellular Domain (NICD) (rabbit polyclonal)   品牌 Millipore

Anti-Notch 1, Notch Intracellular Domain (NICD) (rabbit polyclonal)

货号： 07-1232      产品名称： Anti-Notch 1, Notch Intracellular Domain (NICD) (rabbit polyclonal)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Notch 1 Antibody, NT
Recommended Replacement for: 07-1232 is a recommended replacement for AB5709
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REFERENCES
Regulation of notch1 signaling by nrf2: implications for tissue regeneration.
Nobunao Wakabayashi,Soona Shin,Stephen L Slocum,Elin S Agoston,Junko Wakabayashi,Mi-Kyoung Kwak,Vikas Misra,Shyam Biswal,Masayuki Yamamoto,Thomas W Kensler (2010) Science signaling.3
Species Reactivity Key Applications Host Format Antibody Type
H, M  ELISA, WB, IH(P) Rabbit Serum Polyclonal Antibody
Description:
Anti-Notch 1 Antibody, NT
Replaces:
07-1232 is a recommended replacement for AB5709
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
Notch 1, cleaved N terminal. Only the cleaved intracellular (activated) form is detected. No reactivity is detected against mouse N2, N3 or N4. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form.
Molecular Weight:
~80 kDa
Epitope:
Cleaved N-Terminus
Immunogen:
Synthetic peptide from the N-terminal sequence of the cleaved N intracellular domain (NICD) human Notch 1.
Isotype:
IgG
Background Information:
Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
View All »
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human and mouse. The antibody is also expected to react with rat due to immunogen sequence homology. Other species have not been tested.
Application Notes:
ELISA:
A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of Notch-1 With Citrate pH 6.0 Epitope Retrieval: Uterine Cancer
ELISA: A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.
View All »
Control:
Jurkate Cell Lysates. In fetal tissues, use spleen, brain stem and lung. In adult tissues use mainly lymphoid tissues.
Quality Assurance:
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected cleaved NOTCH1 on 10 μg of Jurkat lysates.
Presentation:
Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, containing 0.1% sodium azide.
Storage Conditions:
Stable for 6 months at -20°C in undiluted aliquots from date o -20°C

07-1233   Anti-Notch 2, Intracellular (rabbit polyclonal)   品牌 Millipore

Anti-Notch 2, Intracellular (rabbit polyclonal)

货号： 07-1233      产品名称： Anti-Notch 2, Intracellular (rabbit polyclonal)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Notch 2 Antibody, intracellular
Recommended Replacement for: 07-1233 is a recommended replacement for AB5711
Close
REFERENCES
The Notch ligand Jagged2 promotes lung adenocarcinoma metastasis through a miR-200-dependent pathway in mice.
Yanan Yang,Young-Ho Ahn,Don L Gibbons,Yi Zang,Wei Lin,Nishan Thilaganathan,Cristina A Alvarez,Daniel C Moreira,Chad J Creighton,Philip A Gregory,Gregory J Goodall,Jonathan M Kurie (2011) The Journal of clinical investigation.121
Species Reactivity Key Applications Host Format Antibody Type
H, M  ELISA, WB, IH(P) Rabbit Serum Polyclonal Antibody
Description:
Anti-Notch 2 Antibody, intracellular
Replaces:
07-1233 is a recommended replacement for AB5711
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
Notch 2, intracellular. The antibody is also expected to react with rat and mouse due to immunogen sequence homology (13/14).
Molecular Weight:
~265 kDa
Epitope:
Intracellular
Immunogen:
Synthetic peptide from amino acids 2396-2409 of human Notch 2.
Isotype:
IgG
Background Information:
Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
View All »
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Reacts with Human and mouse.
Application Notes:
ELISA:
1:30,000-1:90,000 dilution from a previous lot was used in a standard sandwich ELISA assay against the peptide immunogen.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of NOTCH1 With Citrate pH 6.0 Epitope Retrieval: Sarcoma and Uterine Cancer.
View All »
Control:
C2C12 Cell Lysate
Quality Assurance:
Routinely evaluated by Western Blot on C2C12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected NOTCH2 on 10 μg of C2C12 lysates.
Presentation:
Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.1% sodium azide.
Storage Conditions:
Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
View All »
UniProt Number:
Q04721
Entrez Gene Number:
NM_024408.2
Gene Symb -20°C

21180   Cell Meter Fluorimetric Intracellular pH Assay Kit   品牌 AAT

Cell Meter Fluorimetric Intracellular pH Assay Kit

货号： 21180      产品名称： Cell Meter Fluorimetric Intracellular pH Assay Kit   品牌： AAT 规格： 500 Tests 二周到货 生化试验

Cell Meter� Fluorimetric Intracellular pH Assay Kit
Cat # 21180
Storage F/D/L (see storage codes below)
Unit Size 1000 Tests
Ex (nm) 503
Em (nm) 528 
Intracellular pH change are implicated in diverse physiological and pathological processes, including cell proliferation, apoptosis, fertilization, malignancy, multidrug resistance, ion transport, lysosomal storage disorders and Alzheimer抯 disease. The Cell Meter� Fluorimetric Intracellular pH Assay Kit utilizes AAT Bioquest抯 proprietary fluorescent indicator for measuring the relative intracellular pH changes. It is a homogeneous, kinetic, live-cell fluorescent assay that utilizes either a standard procedure or acid-load procedure. The standard protocol is designed for measuring the therapeutic targets of interest with a decrease in intracellular pH upon treatment. The 慉cid-Load� procedure is designed to measure the increase of intracellular pH associated with changes in cellular metabolism due to GPCR activation or growth factor activity. With the 慉cid-Load� procedure ammonium chloride solution is added after the fluorescent pH dye is loaded into cells in a minimum volume. This 慳cid-loading� step is followed by the addition of agonist in a relatively large volume (~4X) of buffer. The sudden volume change initiates an efflux of ammonia (NH3) from the cells causing a rapid decrease in intracellular pH, and thus a decrease in fluorescence signal. The effect of agonist on the subsequent recovery of intracellular pH is measured by the relative fluorescence signal increase.
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     pH and Ion Indicators     Cell Metabolism 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

K4921-100   FTO (human intracellular) ELISA Kit   品牌 Biovision

FTO (human intracellular) ELISA Kit

货号： K4921-100      产品名称： FTO (human intracellular) ELISA Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

FTO (human intracellular) ELISA Kit
Product Overview
Assay Kit Name:
FTO (human intracellular) ELISA Kit
Kit Summary:
Detection method- Absorbance (450 nm)
• Sample type- Cell lysates
• Species reactivity- Mammalian
• Applications- This assay is to be used for the in vitro quantitative determination of human FTO in the range of 0.156 – 10 ng FTO/ml and a detection limit of 50 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
Features & Benefits:
• Simple procedure
• Fast and convenient
• High-throughput adaptable
Kit Components:
• Pre-coated Microtiter Plate
• Wash Buffer (10X)
• Diluent (5X)
• Lysis Buffer (10X)
• Detection Antibody
• Detector 100X (Hrp conjugated anti-IgG)
• Human FTO Standard (lyophilized, 20 ng)
• Human FTO QC Sample (lyophilized)
• TMB Substrate Solution
• Stop Solution
• Plate Sealers
Description:
FTO, Fat mass-and obesity-associated gene, was discovered as a responsible gene causing the mouse ‘fused toes’ mutation. The predicted 502-amino acid Fto protein has a calculated molecular mass of 58 kD and contains an N-terminal bipartite nuclear localization signal. FTO is widely expressed in a variety of human tissues, with highest levels in brain and pancreatic islets. Bioinformatics analysis indicates that FTO shares sequence motifs with iron- and 2-oxoglutarate (2OG)-dependent oxygenases. The FTO (human) (IntraCellular) ELISA Kit is to be used for the in vitro quantitative determination of human FTO in cell lysates or cell-based assays (screening). This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human FTO in cells. A monoclonal antibody specific for FTO has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, FTO is recognized by the addition of a purified polyclonal antibody specific for FTO (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of FTO in the samples. This ELISA is specific for the measurement of natural and recombinant human FTO. It does cross-react with human adiponectin, human RBP4, human Nampt, human vaspin, human progranulin, human resistin, human clusterin, human GPX3, human sirtuin 1, human IDO, human IL-33, human ANGPTL3, human ANGPTL4, human FGF21, mouse progranulin, mouse ANGPTL3, mouse leptin, rat Nampt. The assay range is 0.156 – 10 ng FTO/ml and a detection limit of 50 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
Storage Conditions:
+4°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

+4°C