Anti-Notch 2, Intracellular (rabbit polyclonal)
货号： 07-1233 产品名称： Anti-Notch 2, Intracellular (rabbit polyclonal) 品牌： Millipore 规格： EA 3周到货 生化实验
Anti-Notch 2 Antibody, intracellular
Recommended Replacement for: 07-1233 is a recommended replacement for AB5711
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REFERENCES
The Notch ligand Jagged2 promotes lung adenocarcinoma metastasis through a miR-200-dependent pathway in mice.
Yanan Yang,Young-Ho Ahn,Don L Gibbons,Yi Zang,Wei Lin,Nishan Thilaganathan,Cristina A Alvarez,Daniel C Moreira,Chad J Creighton,Philip A Gregory,Gregory J Goodall,Jonathan M Kurie (2011) The Journal of clinical investigation.121
Species Reactivity Key Applications Host Format Antibody Type
H, M ELISA, WB, IH(P) Rabbit Serum Polyclonal Antibody
Description:
Anti-Notch 2 Antibody, intracellular
Replaces:
07-1233 is a recommended replacement for AB5711
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Specificity:
Notch 2, intracellular. The antibody is also expected to react with rat and mouse due to immunogen sequence homology (13/14).
Molecular Weight:
~265 kDa
Epitope:
Intracellular
Immunogen:
Synthetic peptide from amino acids 2396-2409 of human Notch 2.
Isotype:
IgG
Background Information:
Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Reacts with Human and mouse.
Application Notes:
ELISA:
1:30,000-1:90,000 dilution from a previous lot was used in a standard sandwich ELISA assay against the peptide immunogen.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of NOTCH1 With Citrate pH 6.0 Epitope Retrieval: Sarcoma and Uterine Cancer.
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Control:
C2C12 Cell Lysate
Quality Assurance:
Routinely evaluated by Western Blot on C2C12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected NOTCH2 on 10 μg of C2C12 lysates.
Presentation:
Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.1% sodium azide.
Storage Conditions:
Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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UniProt Number:
Q04721
Entrez Gene Number:
NM_024408.2
Gene Symb -20°C
